Construction and analysis of an SSH cDNA library of early heat-induced genes of<i>Vigna aconitifolia</i>variety RMO-40

Rampuria, Sakshi; Joshi, Uma; Palit, P.; Deokar, Amit; Meghwal, Raju Ram; Mohapatra, Trilochan; Srinivasan, Raji; Bhatt, K. V.; Sharma, Ramavtar

doi:10.1139/g2012-064

Cited by 13 publications

(9 citation statements)

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“…In the current study, VaDJI was isolated from Vigna using an SSR cDNA library and was found to be induced by signalling molecules and abiotic stress treatments ( Rampuria et al., 2012 ). DnaJ proteins are known to be induced by heat, intense light, cold, methyl viologen (MV) and to play roles in signal transduction, development and stress tolerance ( Xia et al., 2014 ; Lee et al., 2018 ; Rampuria et al., 2018 ; Wang et al., 2018a ; Wang et al., 2019a ; Wang et al., 2020 ; Li et al., 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…The full-length VaDJI CDS sequence was amplified from Vigna aconitifolia (RMO-40) using gene-specific primers listed in the earlier cDNA-AFLP study ( Rampuria et al., 2012 ). The PCR product of VaDJI was cloned into a pRT100 vector expression cassette using Apa I and Kpn I restriction sites at the 5′/3′ end of the sequence ( Gautam et al., 2019 ).…”

Section: Methodsmentioning

confidence: 99%

“…This crop is an important biological resource to study the tolerance mechanisms due to its evolved morpho-physiological and gene-reservoir features for drought amelioration ( Iseki et al., 2018 ). A previous study using the construction of suppression subtractive hybridization (SSH) cDNA library in the heat-tolerant genotype of Vigna aconitifolia RMO-40, (moth bean) identified the DnaJ-like protein to be induced upon treatment at an early stage of heat stress (within 5 min) ( Rampuria et al., 2012 ). In line with this observation, the DnaJ family members appeared to be induced in pepper under heat stress conditions ( Fan et al., 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Ectopic expression of DnaJ type-I protein homolog of Vigna aconitifolia (VaDJI) confers ABA insensitivity and multiple stress tolerance in transgenic tobacco plants

et al. 2023

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Reduced crop productivity results from altered plant physiological processes caused by dysfunctional proteins due to environmental stressors. In this study, a novel DnaJ Type-I encoding gene, VaDJI having a zinc finger motif in its C-terminal domain was found to be induced early upon treatment with heat stress (within 5 min) in a heat tolerant genotype of Vigna aconitifolia RMO-40. VaDJI is induced by multiple stresses. In tobacco, ectopic expression of VaDJI reduced ABA sensitivity during seed germination and the early stages of seedling growth of transgenic tobacco plants. Concomitantly, it also improved the ability of transgenic tobacco plants to withstand drought stress by modulating the photosynthetic efficiency, with the transgenic plants having higher Fv/Fm ratios and reduced growth inhibition. Additionally, transgenic plants showed a reduced build-up of H2O2 and lower MDA levels and higher chlorophyll content during drought stress, which attenuated cell damage and reduced oxidative damage. An analysis using the qRT-PCR study demonstrated that VaDJI overexpression is associated with the expression of some ROS-detoxification-related genes and stress-marker genes that are often induced during drought stress responses. These findings suggest a hypothesis whereby VaDJI positively influences drought stress tolerance and ABA signalling in transgenic tobacco, and suggests that it is a potential gene for genetic improvement of drought and heat stress tolerance in crop plants.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Ectopic expression of DnaJ type-I protein homolog of Vigna aconitifolia (VaDJI) confers ABA insensitivity and multiple stress tolerance in transgenic tobacco plants

et al. 2023

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“…4,15 The expression of genes for abiotic stress responsiveness was carried out by various gene expression studies in legumes. 3,13,16 Similarly, in the present study, the expression characterization of selected genes was done by semi-qRT PCR analysis. This was done to select the gene which shows the exponential up-regulation during the time course experiment of drought in moth bean.…”

Section: Assessment Of Drought Stress-responsive Expression Of Moth B...mentioning

confidence: 98%

Identification and characterization of CAT1 gene during drought stress in moth bean [Vigna aconitifolia (Jacq.) Marechal]

Meena¹,

Dogra²,

Sudarsan³

et al. 2022

J. Agric. Ecol.

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Moth bean [Vigna aconitifolia (Jacq.) Marechal] is a drought hardy orphan pulse crop. However, the information related to the identification and characterization of drought stress tolerance genes is very limited. Therefore, the present investigation was formulated to identified and characterized drought tolerant gene(s) from moth bean. Five genes were selected from available expression studies of moth bean and their expression pattern was assessed during time course experiment of drought stress in moth bean. During the time course experiment of drought stress in moth bean, the catalase1 (CAT1) gene was exponentially expressed in up-regulated manner. Thus, CAT1 gene of moth bean was identified as potential candidate gene and validated through Sanger’s sequencing. The genomic sequence CAT1 gene was named as VacoCAT1 and was further characterized using various bioinformatics tools. The VacoCAT1 showed an ORF with incomplete length of 213 bp which encoded 71 amino acids. The coding sequence of VacoCAT1 gene was shown a single exon due to incomplete nature of genomic sequences. The multiple sequence alignment of VacoCAT1 revealed the highly conserved region at 3’ site of the gene as compared to CAT1 gene of other crop species including legumes. The phylogenetic analysis of VacoCAT1 and CAT1 gene of other crop species including legumes revealed three clusters. The cluster VacoCAT1 gene showed close proximity with V. radiata CAT1 in cluster one of phylogenetic tree. The identified and characterized VacoCAT1 gene can be utilized as a genomic resource for enhance drought tolerance in susceptible pulses as well as other crops.

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“…The functional genomic approaches such as genome wide association studies (GWAS) and gene expression profiling using microarrays can catalyse the discovery of novel genes associated to heat stress 6– 8 . In addition, suppression subtractive hybridization (SSH) is another effective and productive technique used for the screening and cloning of the genes/ESTs that express differentially under heat stress 9, 10 . Reverse genetic techniques can improve the understanding of their expression patterns under heat stress.…”

Section: Introductionmentioning

confidence: 99%

Functional genomic approaches to improve crop plant heat stress tolerance

et al. 2019

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Heat stress as a yield limiting issue has become a major threat for food security as global warming progresses. Being sessile, plants cannot avoid heat stress. They respond to heat stress by activating complex molecular networks, such as signal transduction, metabolite production and expressions of heat stress-associated genes. Some plants have developed an intricate signalling network to respond and adapt it. Heat stress tolerance is a polygenic trait, which is regulated by various genes, transcriptional factors, proteins and hormones. Therefore, to improve heat stress tolerance, a sound knowledge of various mechanisms involved in the response to heat stress is required. The classical breeding methods employed to enhance heat stress tolerance has had limited success. In this era of genomics, next generation sequencing techniques, availability of genome sequences and advanced biotechnological tools open several windows of opportunities to improve heat stress tolerance in crop plants. This review discusses the potential of various functional genomic approaches, such as genome wide association studies, microarray, and suppression subtractive hybridization, in the process of discovering novel genes related to heat stress, and their functional validation using both reverse and forward genetic approaches. This review also discusses how these functionally validated genes can be used to improve heat stress tolerance through plant breeding, transgenics and genome editing approaches.

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Construction and analysis of an SSH cDNA library of early heat-induced genes ofVigna aconitifoliavariety RMO-40

Cited by 13 publications

References 49 publications

Ectopic expression of DnaJ type-I protein homolog of Vigna aconitifolia (VaDJI) confers ABA insensitivity and multiple stress tolerance in transgenic tobacco plants

Ectopic expression of DnaJ type-I protein homolog of Vigna aconitifolia (VaDJI) confers ABA insensitivity and multiple stress tolerance in transgenic tobacco plants

Identification and characterization of CAT1 gene during drought stress in moth bean [Vigna aconitifolia (Jacq.) Marechal]

Functional genomic approaches to improve crop plant heat stress tolerance

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