Construction and analysis of the chromosome-level haplotype-resolved genomes of two Crassostrea oyster congeners: Crassostrea angulata and Crassostrea gigas

Qi, Haigang; Cong, Rihao; Wang, Yanjun; Li, Li; Zhang, Guofan

doi:10.1093/gigascience/giad077

Cited by 10 publications

(1 citation statement)

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“…The squared correlation coefficient (r 2 ) was commonly used to quantify the strength of LD, and it rapidly decreased as the distance between SNPs increased, which is consistent with several studies on aquatic animals, such as heat stress in interspecific backcross progenies of channel catfish, metal accumulation in oysters, and the association analysis of fatty acid content in Atlantic salmon [ 44 , 56 ]. Additionally, In C. gigas and C. angulata , when the r 2 value decreased to 0.1, the distance between loci decreased to approximately 600 bp and 1000 bp, respectively [ 57 ]. In our study, the LD decay distance of F 2 population was approximately 500 bp.…”

Section: Discussionmentioning

confidence: 99%

Genome-Wide Association Analysis of Heat Tolerance in F2 Progeny from the Hybridization between Two Congeneric Oyster Species

Du,

Jiang,

Wang

et al. 2023

IJMS

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As the world’s largest farmed marine animal, oysters have enormous economic and ecological value. However, mass summer mortality caused by high temperature poses a significant threat to the oyster industry. To investigate the molecular mechanisms underlying heat adaptation and improve the heat tolerance ability in the oyster, we conducted genome-wide association analysis (GWAS) analysis on the F2 generation derived from the hybridization of relatively heat-tolerant Crassostrea angulata ♀ and heat-sensitive Crassostrea gigas ♂, which are the dominant cultured species in southern and northern China, respectively. Acute heat stress experiment (semi-lethal temperature 42 °C) demonstrated that the F2 population showed differentiation in heat tolerance, leading to extremely differentiated individuals (approximately 20% of individuals die within the first four days with 10% survival after 14 days). Genome resequencing and GWAS of the two divergent groups had identified 18 significant SNPs associated with heat tolerance, with 26 candidate genes located near these SNPs. Eleven candidate genes that may associate with the thermal resistance were identified, which were classified into five categories: temperature sensor (Trpm2), transcriptional factor (Gata3), protein ubiquitination (Ube2h, Usp50, Uchl3), heat shock subfamily (Dnajc17, Dnaja1), and transporters (Slc16a9, Slc16a14, Slc16a9, Slc16a2). The expressional differentiation of the above genes between C. gigas and C. angulata under sublethal temperature (37 °C) further supports their crucial role in coping with high temperature. Our results will contribute to understanding the molecular mechanisms underlying heat tolerance, and provide genetic markers for heat-resistance breeding in the oyster industry.

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Section: Discussionmentioning

confidence: 99%