Construction and application of 3-fucosyllactose whole-cell biosensor for high-throughput screening of overproducers

Li, Qinggang; Liu, Chuan; He, Jinhuai; Liu, Tiantian; Zhang, Wencong; Xie, Zhenzhen; Zong, Jianfei; Li, Yu; Sun, Xue; Lu, Fuping

doi:10.1016/j.biortech.2024.130798

Bioresource Technology

2024

DOI: 10.1016/j.biortech.2024.130798

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Construction and application of 3-fucosyllactose whole-cell biosensor for high-throughput screening of overproducers

Qinggang Li,

Chuan Liu,

Jinhuai He

et al.

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Cited by 2 publications

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Developing a Metabolic Flux Optimized Escherichia coli for Efficient Production of Difucosyllactose

Chen,

Zhu,

Wang

et al. 2024

ACS Sustainable Chem. Eng.

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Developing a Metabolic Flux Optimized Escherichia coli for Efficient Production of Difucosyllactose

Chen,

Zhu,

Wang

et al. 2024

ACS Sustainable Chem. Eng.

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High Throughput Screening of Transcription Factor LysG for Constructing a Better Lysine Biosensor

Li,

Ren,

Liao

et al. 2024

Biosensors

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The biosensors based on transcription factors (TFs) are widely used in high throughput screening of metabolic overproducers. The unsatisfactory performances (narrow detection and dynamic ranges) of biosensors limit their practical application and need more improvement. In this study, using the TF LysG (sensing lysine) as an example, a biosensor optimization method was constructed by growth-coupled screening of TF random mutant libraries. The better the performance of the biosensor, the faster the strain grows under screening pressure. A LysGE15D, A54D, and I164V-based biosensors were obtained, which were about 2-fold of the control in the detection and dynamic ranges. A lysine high-producer was screened effectively using the optimized biosensor with the production at 1.51 ± 0.30 g/L in flasks (2.22-fold of the original strain). This study provided a promising strategy for optimizing TF-based biosensors and was of high potential to be applied in the lysine high-producers screening process.

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Construction and application of 3-fucosyllactose whole-cell biosensor for high-throughput screening of overproducers

Cited by 2 publications

References 42 publications

Developing a Metabolic Flux Optimized Escherichia coli for Efficient Production of Difucosyllactose

Developing a Metabolic Flux Optimized Escherichia coli for Efficient Production of Difucosyllactose

High Throughput Screening of Transcription Factor LysG for Constructing a Better Lysine Biosensor

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