Construction and application of a mass spectral and retention time index database generated from plant GC/EI-TOF-MS metabolite profiles

Wagner, Cornelia; Sefkow, Michael; Kopka, Joachim

doi:10.1016/s0031-9422(02)00703-3

Cited by 271 publications

(198 citation statements)

References 28 publications

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“…We performed recombination experiments, whereby extracts of potato and tomato were subjected to GC-MS investigations, both in isolation then as a stochiometric mixture, to validate that the peak identification for tomato samples was the same as that for potato tuber. This experiment is of particular importance given that different extract compositions can cause so-called "matrix effects" that can result in shifts in relative elution times (for example, see Wagner et al, 2003). Using this strategy, we evaluated whether the relative values determined in the simple tomato extracts could be quantifiably retrieved in the mixed extract.…”

Section: Absolute Quantification Of Metabolites and Validation Of Thementioning

confidence: 99%

Metabolic Profiling of Transgenic Tomato Plants Overexpressing Hexokinase Reveals That the Influence of Hexose Phosphorylation Diminishes during Fruit Development

et al. 2003

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We have conducted a comprehensive metabolic profiling on tomato (Lycopersicon esculentum) leaf and developing fruit tissue using a recently established gas chromatography-mass spectrometry profiling protocol alongside conventional spectrophotometric and liquid chromatographic methodologies. Applying a combination of these techniques, we were able to identify in excess of 70 small-M r metabolites and to catalogue the metabolite composition of developing tomato fruit. In addition to comparing differences in metabolite content between source and sink tissues of the tomato plant and after the change in metabolite pool sizes through fruit development, we have assessed the influence of hexose phosphorylation through fruit development by analyzing transgenic plants constitutively overexpressing Arabidopsis hexokinase AtHXK1. Analysis of the total hexokinase activity in developing fruits revealed that both wild-type and transgenic fruits exhibit decreasing hexokinase activity with development but that the relative activity of the transgenic lines with respect to wild type increases with development. Conversely, both point-by-point and principal component analyses suggest that the metabolic phenotype of these lines becomes less distinct from wild type during development. In summary, the data presented in this paper demonstrate that the influence of hexose phosphorylation diminishes during fruit development and highlights the importance of greater temporal resolution of metabolism.Hexokinase (E.C. 2.7.1.1) catalyzes the phosphorylation of hexoses to form hexose monophosphates. This reaction is especially important in plants because the use of free phosphates is particularly complex in higher plants ( Kruger, 1997). There have been many reports on the presence of glucokinase and hexokinase enzymes in a wide variety of plant species including tomato (Lycopersicon esculentum; Martinez-Barajaz and Randall, 1998), maize (Zea mays; Doehlert, 1989;Schnarrenberger, 1990; Galina et al., 1995), potato (Solanum tuberosum; Renz and Stitt, 1993;Veramendi et al., 1999), pea (Pisum sativum; Turner et al., 1977;Turner and Copeland, 1981) Recently, transgenic manipulations of the activity of hexokinase have been carried out in tomato, potato, and Arabidopsis (Jang et al., 1997; Dai et al., 1999;Veramendi et al., 1999Veramendi et al., , 2002. The results of these manipulations varied greatly between species. Transgenic Arabidopsis seeds that exhibited decreased or increased activities of hexokinase 1 displayed hyposensitive or hypersensitive responses to growth on high (6% [w/v]) Glc containing agar (Jang et al., 1997). The authors concluded that the hexokinase protein acts as a sensor for Glc in an analogous manner to those operating in yeast (Saccharomyces cerevisiae) and that the modulation in the abundance of this sensor led to changes in gene expression that were responsible for the phenotype observed. The overexpression of this Arabidopsis hexokinase isoform in tomato plants led to growth inhibition, reduced photosynthesis, and a...

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Section: Absolute Quantification Of Metabolites and Validation Of Thementioning

confidence: 99%

Metabolic Profiling of Transgenic Tomato Plants Overexpressing Hexokinase Reveals That the Influence of Hexose Phosphorylation Diminishes during Fruit Development

et al. 2003

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“…Phenylpropanoids and fatty acids profiling was performed as detailed previously [16,11] by gas chromatography coupled with electron impact ionization/time-of-flight mass spectrometry (GC-EI/TOF-MS) using an Agilent 6890N24 gas chromatograph (Agilent Technologies, Böblingen, Germany; http://www.agilent.com) with 1/30 split and splitless injection of 1 µl onto a Factor Four VF-5 ms capillary column, 30 m length, 0.25 mm inner diameter, 0.25 µm film thickness (Varian-Agilent Technologies, Waldbronn, Germany) which was connected to a Pegasus III time-of-flight mass spectrometer (LECO Instrumente GmbH, Mönchengladbach, Germany; http://www.leco.de).…”

Section: Gc-ie-tof-ms Chromatographymentioning

confidence: 99%

Phenylpropanoids and Fatty Acids Levels in Roots and Leaves of Datura Stramonium and Datura Innoxia

Rahmoune

Zerrouk

Morsli

et al. 2017

Int J Pharm Pharm Sci

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Objective: The aim of this research was to determine and compare phenylpropanoids and fatty acids composition in two plant species, Datura innoxia and Datura stramonium. Methods:Phenylpropanoids and fatty acids composition in leaves and roots extracted from Datura innoxia and Datura stramonium, grown under greenhouse conditions, was analyzed by gas chromatography-electron impact/time of flight-mass spectrometry (GC-EI/TOF-MS) chromatography techniques. Analyses were carried out at the Max Planck Institute for Molecular Plant Physiology of Golm (Germany). Results:We revealed that Datura stramonium (DS) contains hydroxy-hexanedioic acid while hexanoic acid was found in Datura innoxia (DI). Also, two fatty acids are common to both Datura species, hexadecanoic acid and octadecanoic acid, with an almost equal rate between leaves and roots. However, phenylpropanoids composition revealed eight compounds; luteolin, quercetin, trans-caffeic acid, trans-ferulic acid, cis-caffeic acid, cis-4-hydroxy-cinnamic acid, trans-4-hydroxy-cinnamic acid and trans-sinapic acid in DI. However, in DS, five compounds were detected: luteolin, quercetin, trans-caffeic acid, trans-ferulic acid and dihydroferulic acid. Also in both Datura species, phenylpropanoids concentration in leaves was significantly higher than in the roots. Conclusion:Our results showed a difference in phenylpropanoids and fatty acids compositions between the two Datura species, with a significantly higher concentration of phenylpropanoids in Datura innoxia than in Datura stramonium

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“…The supernatant was then concentrated to an aqueous phase under N 2 and lyophilized. The dried samples were derivatized with methoxyamine hydrochloride in pyridine (50 lL, 20 mg/mL), and subsequently with N-methyl-N-trimethylsilyltrifluoroacetamide (MSTFA) (80 lL) (Fiehn et al, 2000;Wagner et al, 2003;Weckwerth et al, 2004;Lisec et al, 2006). A mixture of C 11 to C 36 n-alkanes was used for the determination of retention time indices.…”

Section: Extraction and Gc-tof-ms Analysis Of Metabolitesmentioning

confidence: 99%

“…GC-MS gives possibilities of analyzing different classes of compounds, including organic and amino acids, sugars, sugar alcohols, sterols, phosphorylated and lipophilic compounds (Wagner et al, 2003;Lisec et al, 2006). However, LC-MS is also a powerful technology to separate and analyze semi-polar secondary metabolites that can comprise large and unique groups of compounds in plants (Brown et al, 2003;von Roepenack-Lahaye et al, 2004;Stobiecki et al, 2006;de Vos et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Metabolite profiling of Arabidopsis seedlings in response to exogenous sinalbin and sulfur deficiency

et al. 2011

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Construction and application of a mass spectral and retention time index database generated from plant GC/EI-TOF-MS metabolite profiles

Cited by 271 publications

References 28 publications

Metabolic Profiling of Transgenic Tomato Plants Overexpressing Hexokinase Reveals That the Influence of Hexose Phosphorylation Diminishes during Fruit Development

Metabolic Profiling of Transgenic Tomato Plants Overexpressing Hexokinase Reveals That the Influence of Hexose Phosphorylation Diminishes during Fruit Development

Phenylpropanoids and Fatty Acids Levels in Roots and Leaves of Datura Stramonium and Datura Innoxia

Metabolite profiling of Arabidopsis seedlings in response to exogenous sinalbin and sulfur deficiency

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