Construction and Application of MALDI-TOF Mass Spectrometry for the Detection of Haemophilus parasuis

Abstract

To construct a protein fingerprint database of Haemophilus parasuis (H. parasuis), thus improving its clinical diagnosis efficiency. A total of 15 H. parasuis standard strains were collected to establish a protein fingerprint database of H. parasuis using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), and the effects of different culture media and culture time on the quality and identification results of the protein fingerprint were investigated. The results showed that tryptone soy agar (TSA) and tryptone soy broth (TSB) media and different incubation times had no significant effect on the characteristic peaks of the protein profiles. In addition, 18 clinical isolates were used to compare the identification results of the self-built protein fingerprint database, PCR detection, and basic database. Only one strain was identified in the original VITEK-MS system database, while the self-made protein fingerprint database of H. parasuis was 100% accurate for the detection of 18 clinical isolate strains. The protein fingerprint database of H. parasuis built by our laboratory is suitable for rapid clinical diagnosis of H. parasuis, due to its high accuracy, efficiency, and strong specificity.

Expression Analysis of Outer Membrane Protein HPS_06257 in Different Strains of Glaesserella parasuis and Its Potential Role in Protective Immune Response against HPS_06257-Expressing Strains via Antibody-Dependent Phagocytosis

“…The 13 G. parasuis reference strains for this study were obtained from the China Institute of Veterinary Drug Control [ 16 ]. They were representative of 13 different serovars: serovar 1 (as Glaesserella parasuis is formerly known as Haemophilus parasuis , in this study, Glaesserella parasuis serovar 1, simply named HPS1), serovar 2 (HPS2), serovar 3 (HPS3), serovar 4 (HPS4), serovar 5 (HPS5), serovar 6 (HPS6), serovar 8 (HPS8), serovar 9 (HPS9), serovar 10 (HPS10), serovar 11 (HPS11), serovar 13 (HPS13), serovar 14 (HPS14), and serovar 15 (HPS15).…”

Retracted: Construction and Application of MALDI-TOF Mass Spectrometry for the Detection of Haemophilus parasuis

“…This article has been retracted by Hindawi, as publisher, following an investigation undertaken by the publisher [ 1 ]. This investigation has uncovered evidence of systematic manipulation of the publication and peer-review process.…”