Construction and characterization of a partial binary bacterial artificial chromosome (BIBAC) of Agave tequilana var. azul (2X) and its application for gene identification

ez, Tamayo Ordontilde; M,; Zapata, Rodriacute guez; L, C; Teyer, Saacute nchez; Ma, F L

doi:10.5897/ajb12.2041

Cited by 4 publications

(5 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ribosomal copy number was determined by using quantitative capillary electrophoresis with primers obtained from a consensus sequence derived from several plant ribosomal sequences, specifically the Liliopsida class and Asparagaceae family for 5S and 18S rDNA, respectively. These primers have recently been used to characterize a binary genomic library of A. tequilana Weber (Tamayo-Ordóñez et al 2012). Primer sequences are as follows: 5S (FW 5′-CGATCATACCAGCACTAAAGCACC-3′ and RV 5′-ATGCAACACGAGGACTTCCCAG-3′) and 18S (FW 5′-CGGCTACCACATCCAAGGAA-3′ and RV 5′-GCTGGA ATTACCGCGGCT-3′).…”

Section: Ribosomal Gene Copy Numbermentioning

confidence: 99%

Comparative Characterization of Ribosomal DNA Regions in Different Agave Accessions with Economical Importance

2015

View full text Add to dashboard Cite

Two ribosomal DNA regions (5S and 18S) were characterized in three economically important species of Agave Linnaeus, 1753 namely Agave tequilana Weber, 1902; Agave angustifolia Haworth, 1915; and Agave fourcroydes Lemaire, 1864 which are used to produce several products such as tequila, mezcal, and hard fibers. Characterization included Agave L. accessions with different ploidy levels (2n=2x=60 to 2n=6×=180) in order to relate this factor with copy number, haplotype number, expression profile, and predictable functionality of ribosomal DNA (rDNA) sequences. Only total rDNA copy number (5S and 18S) was related with ploidy level. Main differences were found in the 5S rDNA gene since it exhibited different genetic traits of Agave L. accession. In this gene, four different allelic groups (I, 105; II, 107; III, 110; and IV, 111 bp) were detected, which have probably evolved separately, thus exhibiting different expression profiles and different haplotype occurrence. Allelic groups III and IV exhibit the highest number of total and expressed copies in all Agave L. accessions. Nonredundant haplotypes were probably more functional in these allelic groups. Differences between the Agave L. accessions were more clearly observed in the most cultivated accession, A. tequilana (2n=2×=60), where the allelic group III shows non-redundant haplotypes and is transcriptionally upregulated suggesting a different evolutionary pressure on this Agave L. accession.

show abstract

Section: Ribosomal Gene Copy Numbermentioning

confidence: 99%

Comparative Characterization of Ribosomal DNA Regions in Different Agave Accessions with Economical Importance

2015

View full text Add to dashboard Cite

show abstract

“…[73][74][75] It is hoped that future studies will identify essential genetic and biochemical mechanisms beneficial to biotechnology geared toward engineering drought-and disease-tolerant C 3 and C 4 plants, and even interspecies agaves of interest. 76 Nevertheless, in silico analyses have already identified potential cellulase synthases, glucanases, and sucrose synthases that could be utilized in fiber production. These are intriguing findings, as one of the aims for agave crops is the reduction of time needed for cultivation and maintaining yield and quality of agave-based cellulose materials.…”

Section: Agave Drought Tolerance and Synthetic Biologymentioning

confidence: 99%

“…At present, there is scarce information on the genomic organization of the Agave genus, and also a few transcriptome and proteomic analyses of Agave species, which makes it difficult to identify putative protein‐coding genes that respond to drought, heat stress, and flowering 73–75 . It is hoped that future studies will identify essential genetic and biochemical mechanisms beneficial to biotechnology geared toward engineering drought‑ and disease‐tolerant C 3 and C 4 plants, and even interspecies agaves of interest 76 . Nevertheless, in silico analyses have already identified potential cellulase synthases, glucanases, and sucrose synthases that could be utilized in fiber production.…”

Section: Agave Drought Tolerance and Synthetic Biologymentioning

confidence: 99%

Agave: a natural renewable resource with multiple applications

et al. 2020

View full text Add to dashboard Cite

Agaves are a group of succulent plants that thrive in arid or semiarid environments. Indeed, genes associated with their resilience are a potential resource for genetic engineering of other agronomically important crops grown in adverse climates. Agave is mainly used for the production of distilled (spirits) and non-distilled alcoholic beverages, including tequila, mezcal, bacanora, raicilla, and pulque, all of which have special connections to Mexican history and culture, and contribute to the Mexican economy. In recent years, there has been growing interest to maximize the use of agave plant materials for other purposes, as the bulk of their biomass pre-and post-production is wasted. In traditional practice, during the passage from fields to factories, only agave cores are used, and the leaves and bagasse are not always harnessed. To place this in perspective, during the period from 2010 to 2019, 2674.7 × 10 6 L of tequila was produced in Mexico, which required 9 607 400 tons of agave cores. This generated approximately the same amount of leaves and 3 842 960 tons of bagasse. The economic base of agave plants can be expanded if expended biomass could be transformed into products that are useful for applications in food, forage, ensilage, agriculture, medicine, energy, environment, textiles, cosmetics, and esthetics. This review focuses on the current utility of agave plants, as well as our perspective for future studies and uses of this formidable plant.

show abstract

“…Despite the emphasized latent importance of Agave crops for several biotechnological areas, development of their potential has been hampered by scarcity of knowledge about its genomic structure and organization . Very few studies are available about isolation and identification of the protein and enzyme coding genes participating in the synthetic pathways involved in production of compounds of biotechnological interest.…”

Section: Introductionmentioning

confidence: 99%

“…Despite the emphasized latent importance of Agave crops for several biotechnological areas, 19,26 development of their potential has been hampered by scarcity of knowledge about its genomic structure and organization. 46 Very few studies are available about isolation and identification of the protein and enzyme coding genes participating in the synthetic pathways involved in production of compounds of biotechnological interest. Biotechnological production in Agave might be enhanced by identifying and selecting genes of promising characteristics, selection from wild populations of elite biotypes with commercially interesting features, and application of genetic engineering tools-such as homologous or heterologous transformation-for molecular marker aided breeding.…”

mentioning

confidence: 99%

Review and in silico analysis of fermentation, bioenergy, fiber, and biopolymer genes of biotechnological interest inAgaveL. for genetic improvement and biocatalysis

Tamayo‐Ordóñez

Ayil‐Gutiérrez

Tamayo-Ordóñez

et al. 2018

Biotechnology Progress

View full text Add to dashboard Cite

Several of the over 200 known species of Agave L. are currently used for production of distilled beverages and biopolymers. The plants live in a wide range of stressful environments as a result of their resistance to abiotic stress (drought, salinity, and extreme temperature) and pathogens, which gives the genus potential for germplasm conservation and biotechnological applications that may minimize economic losses as a result of the global climate change. However, the limited knowledge in the genus of genome structure and organization hampers development of potential improved biotechnological applications by means of genetic manipulation and biocatalysis. We reviewed Agave and plant sequences in the GenBank NCBI database for identifying genes with biotechnological potential for fermentation, bioenergy, fiber improvement, and in vivo plant biopolymer production. Three-dimensional modeling of enzyme structures in plant accessions revealed structural differences in sucrose 1-fructosyltransferase, fructan 1-fructosyltransferase, fructan exohydrolase (1-FEH), cellulose synthase (CES), and glucanases (EGases) with possible effects in fructan, sugar, and biopolymer production. Although the coding genes of FEH and enzymes involved in biopolymer production (CES, sucrose synthase, and EGases) remain unidentified in Agave L., our results could aid isolation of such genes in Agave. By comparing nucleotide and amino acid sequences in accessions of Agave and other plants, knowledge may be gained about transcriptional regulation and enzymatic activity factors. Future study is needed of biotechnological application of Agave genes for crop breeding aided by genetic engineering and biocatalysis.

show abstract

Construction and characterization of a partial binary bacterial artificial chromosome (BIBAC) of Agave tequilana var. azul (2X) and its application for gene identification

Cited by 4 publications

References 41 publications

Comparative Characterization of Ribosomal DNA Regions in Different Agave Accessions with Economical Importance

Comparative Characterization of Ribosomal DNA Regions in Different Agave Accessions with Economical Importance

Agave: a natural renewable resource with multiple applications

Review and in silico analysis of fermentation, bioenergy, fiber, and biopolymer genes of biotechnological interest inAgaveL. for genetic improvement and biocatalysis

Contact Info

Product

Resources

About