Construction and Characterization of a<i>Mycobacterium tuberculosis</i>Mutant Lacking the Alternate Sigma Factor Gene,<i>sigF</i>

Chen, Ping; Ruíz, Rafael; Li, Qing; Silver, Richard F.; Bishai, William R.

doi:10.1128/iai.68.10.5575-5580.2000

Cited by 134 publications

(124 citation statements)

References 41 publications

Supporting

Mentioning

113

Contrasting

Unclassified

Order By: Relevance

“…One of the proteins that interact with OsPUM1 in rice is a homologue of sigma factor F (SigF) inhibitor. In bacteria, this protein had been reported to be involved in the regulation of tolerance and susceptibility to rifampin, a drug 18 . However, no characterization of the rice SigF orthologue has been reported.…”

Section: Discussionmentioning

confidence: 99%

Proteins that interact with rice pumilio 1

Sugiharti¹,

Azizi²,

Su’udi³

2013

ScienceAsia

View full text Add to dashboard Cite

ABSTRACT:The N-terminal region of rice pumilio 1 fused with the binding domain (BD-OsPUM1) was used as a bait construct in yeast two-hybrid screening with a rice cDNA library as prey. Several interacting proteins were screened in a stringent media and tested with beta-galactose filter assay. The nucleotide sequences encoding interacting proteins were determined and annotated according to the rice genome database. These proteins are sigma factor F inhibitor, RPL18C, RUBQ2, RPL24A, RCY1, small nuclear ribonucleoprotein G, transferase hexapeptide repeat-containing protein, dormancyassociated protein, and putative expressed proteins with the accessions NP 001067038 and EEE55952. This study suggested that OsPUM1 protein is associated with several biological processes involved in morphology determination, protein folding and plant immunity.

show abstract

Section: Discussionmentioning

confidence: 99%

Proteins that interact with rice pumilio 1

Sugiharti¹,

Azizi²,

Su’udi³

2013

ScienceAsia

View full text Add to dashboard Cite

show abstract

“…This has enabled dissection of the enzymatic apparatus in response to altered environmental conditions. Altered genes include sigma factors [39,40] and PGRS-encoding genes [41], as well as activation of alternative pathways in the citrate cycle to allow metabolism of the abundant lipids in the necrotic centre of granulomas [42,43] and general adaptation to low oxygen tension [44]. More recently, in vitro studies identified a regulon consisting of two sensor kinases, DosS and DosT, under the control of the transcription factor DosR, which sense and control the host response to hypoxia and nitric oxide stress [45][46][47], thus enabling M. tuberculosis to adapt to local conditions during latency [48] (Figure 5).…”

Section: Transition Of M Tuberculosis Into a Dormant State Within Grmentioning

confidence: 99%

New insights into the function of granulomas in human tuberculosis

Ulrichs

Kaufmann

2005

The Journal of Pathology

353

337

View full text Add to dashboard Cite

The human tuberculous granuloma provides the morphological framework for local immune processes central to the outcome of tuberculosis. This review article describes investigations on human lung granulomas aimed at better understanding the regional host response and counter-measures to Mycobacterium tuberculosis. These findings lead to a revised view of the regional immune response in human tuberculosis. Novel insights into this dynamic cross-talk form the basis of novel intervention strategies.

show abstract

“…In previous work, our laboratory and others have implicated several alternative sigma factors in the mycobacterial response to a variety of stresses, most notably oxidative stress (13, 17-19, 24, 34). A role for alternative sigma factor-regulated gene expression in stationary-phase adaptation and in vivo replication in late-stage infections in mice has also been demonstrated (5,11).…”

mentioning

confidence: 99%

Transcription Regulation by the Mycobacterium tuberculosis Alternative Sigma Factor SigD and Its Role in Virulence

et al. 2004

View full text Add to dashboard Cite

Mycobacterium tuberculosis, an obligate mammalian pathogen, adapts to its host during the course of infection via the regulation of gene expression. Of the regulators of transcription that play a role in this response, several alternative sigma factors of M. tuberculosis have been shown to control gene expression in response to stresses, and some of these are required for virulence or persistence in vivo. For this study, we examined the role of the alternative sigma factor SigD in M. tuberculosis gene expression and virulence. Using microarray analysis, we identified several genes whose expression was altered in a strain with a sigD deletion. A small number of these genes, including sigD itself, the gene encoding the autocrine growth factor RpfC, and a gene of unknown function, Rv1815, appear to be directly regulated by this sigma factor. By identifying the in vivo promoters of these genes, we have determined a consensus promoter sequence that is putatively recognized by SigD. The expression of several genes encoding PE-PGRS proteins, part of a large family of related genes of unknown function, was significantly increased in the sigD mutant. We found that the expression of sigD is stable throughout log phase and stationary phase but that it declines rapidly with oxygen depletion. In a mouse infection model, the sigD mutant strain was attenuated, with differences in survival and the inflammatory response in the lung between mice infected with the mutant and those infected with the wild type.Mycobacterium tuberculosis is an obligate mammalian pathogen that is believed to infect roughly one-third of the world's population (33). While capable of causing disease in a substantial proportion of those infected, resulting in approximately eight million cases of active tuberculosis in the world each year, this bacillus causes an asymptomatic infection in most individuals. After an initial period of rapid replication, the infection is typically contained by the host immune system, resulting in the apparent eradication of the infection in some individuals but in the persistence of small numbers of bacteria in others, resulting in asymptomatic chronic infections. These latent infections may subsequently become active, often in the setting of decreased host immunity, with increased bacterial replication and extensive tissue damage.During these several stages of infection, M. tuberculosis encounters a changing host environment, in response to which the bacillus must activate defense and repair mechanisms and reprogram its physiology to ensure survival. The large number of putative transcription regulators identified in the M. tuberculosis genome sequence indicate that much of the regulation required for these adaptations by M. tuberculosis occurs at the level of transcription (6). Among the transcription regulators that have been implicated in these processes are the alternative sigma factors of this organism, 12 of which are encoded in the M. tuberculosis genome. In previous work, our laboratory and others have implicated sev...

show abstract

Construction and Characterization of aMycobacterium tuberculosisMutant Lacking the Alternate Sigma Factor Gene,sigF

Cited by 134 publications

References 41 publications

Proteins that interact with rice pumilio 1

Proteins that interact with rice pumilio 1

New insights into the function of granulomas in human tuberculosis

Transcription Regulation by the Mycobacterium tuberculosis Alternative Sigma Factor SigD and Its Role in Virulence

Contact Info

Product

Resources

About