Construction and characterization of porcine single-chain fragment variable antibodies that neutralize transmissible gastroenteritis virus in vitro

Zhang, Fanqing; Chen, Yuxue; Liu, Yang; Zhu, Jingde

doi:10.1007/s00705-019-04156-6

Cited by 10 publications

(3 citation statements)

References 49 publications

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“…Neutralizing monoclonal antibodies (mAbs) that target the RBD on the S protein of SARS-CoV-2 have shown the potential for both therapeutic and prophylactic applications, and several SARS-CoV-2 mAbs are currently poised to enter into human clinical trials [ 211 , 212 , 213 ]. Neutralizing antibodies targeting the S protein have been studied in various animal CoVs: PEDV and TGEV [ 214 , 215 , 216 , 217 , 218 ], BCoV [ 219 ] and IBV [ 220 , 221 ]. Monoclonal antibodies targeting other CoV proteins have also been investigated, such as four of the six mAbs against BCoV E2 and E3 glycoproteins exhibited neutralization activity in vivo [ 222 ].…”

Section: Therapeutics Against Sars-cov-2 and The Animal Coronavirumentioning

confidence: 99%

Section: Therapeutics Against Sars-cov-2 and The Animal Coronavirusesmentioning

confidence: 99%

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Drawing Comparisons between SARS-CoV-2 and the Animal Coronaviruses

Ghosh

Malik

2020

Microorganisms

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The COVID-19 pandemic, caused by a novel zoonotic coronavirus (CoV), SARS-CoV-2, has infected 46,182 million people, resulting in 1,197,026 deaths (as of 1 November 2020), with devastating and far-reaching impacts on economies and societies worldwide. The complex origin, extended human-to-human transmission, pathogenesis, host immune responses, and various clinical presentations of SARS-CoV-2 have presented serious challenges in understanding and combating the pandemic situation. Human CoVs gained attention only after the SARS-CoV outbreak of 2002–2003. On the other hand, animal CoVs have been studied extensively for many decades, providing a plethora of important information on their genetic diversity, transmission, tissue tropism and pathology, host immunity, and therapeutic and prophylactic strategies, some of which have striking resemblance to those seen with SARS-CoV-2. Moreover, the evolution of human CoVs, including SARS-CoV-2, is intermingled with those of animal CoVs. In this comprehensive review, attempts have been made to compare the current knowledge on evolution, transmission, pathogenesis, immunopathology, therapeutics, and prophylaxis of SARS-CoV-2 with those of various animal CoVs. Information on animal CoVs might enhance our understanding of SARS-CoV-2, and accordingly, benefit the development of effective control and prevention strategies against COVID-19.

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Section: Therapeutics Against Sars-cov-2 and The Animal Coronavirumentioning

confidence: 99%

Section: Therapeutics Against Sars-cov-2 and The Animal Coronavirusesmentioning

confidence: 99%

Drawing Comparisons between SARS-CoV-2 and the Animal Coronaviruses

Ghosh

Malik

2020

Microorganisms

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“…Recently, a porcine scFv antibody has been identified and characterized, having in vitro TGEV neutralizing ability. The therapeutic potential of this novel binder is promising in the control of porcine viral gastroenteritis [63]. Similarly, high-affinity nanobodies targeting Nsp4 and Nsp9 protein of PRRSV with therapeutic potential have been isolated and characterized [64,65].…”

Section: Veterinary Therapeuticsmentioning

confidence: 99%

Construction of Antibody Phage Libraries and Their Application in Veterinary Immunovirology

Bashir

Paeshuyse

2020

Antibodies

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Antibody phage display (APD) technology has revolutionized the field of immunovirology with its application in viral disease diagnostics and antiviral therapy. This robust and versatile technology allows the expression of an antibody fused to a phage coat protein on the surface of a filamentous phage. The DNA sequence coding for the antibody is packaged within the phage, linking the phenotype to genotype. Antibody phage display inherits the ability to rapidly generate and modify or improve high-affinity monoclonal antibodies, rendering it indispensable in immunology. In the last two decades, phage-display-derived antibodies have been extensively used in human medicine as diagnostic and therapeutic modalities. Recently, they are also gaining significant ground in veterinary medicine. Even though these advancements are mainly biased towards economically important animals such as chicken, cattle, and pigs, they are laying the foundation of fulfilling the unmet needs of veterinary medicine as antibody-based biologics in viral diagnostics, therapeutics, and immunoprophylaxis. This review provides a brief overview of the construction of antibody phage libraries and their application in diagnosis, prevention, and control of infectious viral diseases in veterinary medicine in detail.

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Cell-penetrating porcine single-chain antibodies (transbodies) against nonstructural protein 1β (NSP1β) of porcine reproductive and respiratory syndrome virus inhibit virus replication

et al. 2023

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Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), causes porcine reproductive and respiratory syndrome (PRRS) worldwide, especially among domestic pigs with enormous economic impact for the pig industry. Current vaccines confer limited effectiveness while no direct-acting anti-PRRS is available. Non-structural protein (NSP) 1β, a cysteine-like protease (CL Pro ) of PRRSV is pivotal for viral polyprotein processing, subgenomic RNA synthesis and evasion of host innate immunity. Therefore, agent that interferes with the NSP1β bioactivities should lead to the virus replication inhibition. In this study, a porcine scFv-phage display library was constructed and used as a tool for production of NSP1βspeci c porcine scFvs (pscFvs). The pscFvs to NSP1β were linked to a cell-penetrating peptide to form cell-penetrating pscFvs (transbodies). The transbodies could internalize and inhibit PRRSV replication in the infected cells. Computerized-simulation indicated that the effective pscFvs used several residues in multiple complementarity determining regions (CDRs) to interact with many residues in the CL Pro and Cterminal motifs, which might explain the mechanism of the pscFvs-mediated virus replication inhibition.Although experiments are needed to verify the anti-viral mechanism of the transbodies, current data provide evidence for developing the transbodies further for treatment and prevention of PRRSV infection.

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Construction and characterization of porcine single-chain fragment variable antibodies that neutralize transmissible gastroenteritis virus in vitro

Cited by 10 publications

References 49 publications

Drawing Comparisons between SARS-CoV-2 and the Animal Coronaviruses

Drawing Comparisons between SARS-CoV-2 and the Animal Coronaviruses

Construction of Antibody Phage Libraries and Their Application in Veterinary Immunovirology

Cell-penetrating porcine single-chain antibodies (transbodies) against nonstructural protein 1β (NSP1β) of porcine reproductive and respiratory syndrome virus inhibit virus replication

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