Construction and comparison of open tubular reactors for post-column reaction detection in liquid chromatography

Selavka, Carl M.; Jiao, Kai Sheng.; Krull, Ira S.

doi:10.1021/ac00144a048

Cited by 58 publications

(27 citation statements)

References 16 publications

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“…5). This was in agreement with previous observations (Selavka et al, 1987), and was the result of increased band broadening that occurred inside the KOT during extended periods of incubation. The on-line incubation time between the samples and the substrate was 3.5 min based on the length of the KOT used.…”

Section: Noncompetitive Flow Injection Immunoassaysupporting

confidence: 94%

Immunodetection Approaches and High-Performance Immunoaffinity Chromatography for an Analogue of Bovine Growth Hormone Releasing Factor at Trace Levels

Strong

Cho

Fisher

et al. 1996

Biomed. Chromatogr.

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Section: Noncompetitive Flow Injection Immunoassaysupporting

confidence: 94%

Immunodetection Approaches and High-Performance Immunoaffinity Chromatography for an Analogue of Bovine Growth Hormone Releasing Factor at Trace Levels

Strong

Cho

Fisher

et al. 1996

Biomed. Chromatogr.

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“…The manifold consists of one 8-channel peristaltic pump (Dynamax, Rannin), two electronically actuated 6-port valves (VICI, Valco Instruments), three 1-m mixing coils, one 3-m reaction coil, a preconcentration column (made of chlorotrifluoroethylene [KEL-F]) with a tapered inner chamber (85 µL) and nonmetal frits (1-cm mini-column; Global-FIA), a variable wavelength spectrometer (USB-2000; Ocean Optics), a tungsten light supply (LS-1; Ocean Optics), two optical fibers with SMA-905 connectors (diameter 100 µm, length 1 m; Ocean Optics), a cuvette holder with SMA adaptors (CUV-UV; Ocean Optics), and a 10-mm pathlength quartz flow cell (Starna Cells). The mixing and reaction coils (knotted as described by Selavka et al [1987]) and all other manifold tubing are 0.5-mm i.d. PFA Teflon tubing (Upchurch Scientific).…”

Section: Materials and Proceduresmentioning

confidence: 99%

“…The sensitivity of a kinetic catalytic method is enhanced by increasing reaction times, which in FI is accomplished through longer reaction coils or slower flow rates. However, increasing the length of the reaction coil increases not only the time of analysis, but also dispersion, which lowers sensitivity, while slower flow rates result in a decrease in mixing efficiency (Selavka et al 1987). …”

Section: Introductionmentioning

confidence: 99%

Catalytically enhanced spectrophotometric determination of manganese in seawater by flow‐injection analysis with a commercially available resin for on‐line preconcentration

Aguilar-Islas

Bruland

2006

Limnology & Ocean Methods

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The sensitive, laboratory-and ship-based, flow-injection (FI) method for the determination of dissolved manganese in seawater developed by Resing and Mottl (Anal. Chem. 1992;64:2682-2687) has been significantly modified and improved by incorporating five significant changes. The three major changes are the use of a commercially available iminodiacetate (IDA) resin (Toyopearl AF-chelate 650M) in place of 8-hydroxyquinoline for on-line preconcentration of manganese and matrix removal, the addition of nitrilotriacetic acid as an activator ligand which increases sensitivity by a factor of 7 and decreases the limit of detection, and the on-line buffering of acidified samples before column loading. Two minor improvements include use of the more soluble sodium periodate in place of potassium periodate and elimination of Brij-35 surfactant. To accommodate these changes, the pH of samples was adjusted to 8.5 (vs. 7.8), a higher acid concentration was required to elute the Mn from the IDA resin, and stronger reaction buffer was required to neutralize the acid. The accuracy of the method was evaluated with the use of NASS-4 standard seawater and by a comparison study of samples from the California coast that were analyzed by this method and an FI method coupled to inductively coupled plasma sector field mass spectrometry detection. The detection limit and precision of the method depend on the amount of sample preconcentrated onto the column. By preconcentrating 1.6 mL of sample, a detection limit of 0.03 nM (3 times the standard deviation of a blank) and precision of 3.2% (as percent relative standard deviation) were obtained. The method was used on board ship to determine dissolved manganese in coastal waters off Oregon and Washington.

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“…The HPLC column was a Supelcosil ABZ Plus C 18 column (150 Â 2.1 mm i.d., 5 mm particle size; Supelco, Bellefonte, PA). The HPLC conditions for separation of biotin (Selavka et al, 1987). This was used to provide the required incubation time for the postcolumn reaction to occur.…”

Section: Methodsmentioning

confidence: 99%

Post-column reaction detection of biotin in human plasma ultrafiltrate based on laser-induced fluorescence energy transfer in the far-red spectral region

Shahdeo

Anderson

Karnes

2000

Biomed. Chromatogr.

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High performance liquid chromatography followed by post-column reaction detection in the far-red spectral region provides added sensitivity and selectivity. A homogeneous fluorescence energy transfer assay in the competitive mode based on the binding of biotin and streptavidin was developed as an on-line post-column reaction detection system. The labels used for energy transfer were R-Phycoerythrin conjugated to biotin and Cyanine 5 labeled with streptavidin. The energy transfer peak was measured at 670 nm and excitation was achieved using the 488 nm line of an argon ion laser. The biotin concentration in plasma ultrafiltrate ranged from 0.024 to 6.12 ng/mL (n = 6). The precision of the two controls, 0.24 and 2. 44 ng/mL, was found to be 18.70% and 9.92% relative standard deviation respectively. Accuracy was 10.47% and 1.95% difference from spiked, respectively (n = 6). The limit of detection was 21.70 pg/mL (8.90 x 10(-11)M) calculated based on a factor of 2x the standard deviation of the blank (n = 6). The correlation coefficient for the calibration curve was found to be 0.9995. Recovery from plasma ultrafiltrate at 2.44 ng/mL was 103.40% (n = 6). Detection selectivity was indicated by the absence of background fluorescence in six different plasma samples collected from six individual donors. Endogenous levels were detected in two of the six pools of plasma ultrafiltrates.

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Construction and comparison of open tubular reactors for post-column reaction detection in liquid chromatography

Cited by 58 publications

References 16 publications

Immunodetection Approaches and High-Performance Immunoaffinity Chromatography for an Analogue of Bovine Growth Hormone Releasing Factor at Trace Levels

Immunodetection Approaches and High-Performance Immunoaffinity Chromatography for an Analogue of Bovine Growth Hormone Releasing Factor at Trace Levels

Catalytically enhanced spectrophotometric determination of manganese in seawater by flow‐injection analysis with a commercially available resin for on‐line preconcentration

Post-column reaction detection of biotin in human plasma ultrafiltrate based on laser-induced fluorescence energy transfer in the far-red spectral region

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