Construction and immunogenicity of a recombinant fowlpox virus containing the capsid and 3C protease coding regions of foot-and-mouth disease virus

Zheng, Ming‐Hua; Jin, Ningyi; Zhang, Hongyong; Jin, Minglan; Lu, Huijun; Ma, Minxiao; Chang, Li; Yin, Gefen; Wang, Ruilin; Liu, Qi

doi:10.1016/j.jviromet.2006.05.019

Cited by 36 publications

(15 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These vaccines have proven useful in preventing the disease but present important constraints, such as the risk of virus escape from production plants and the difficulty in distinguishing infected animals from uninfected vaccinates. New vaccine strategies aimed at overcoming these problems have been developed, including subunit vaccines consisting of VP1 protein or chemically synthesized peptides (57,61), different DNA constructs expressing viral immunogens (6,8,15), and recombinant viruses (25,35,44,54,63). Live attenuated candidate vaccines for FMD lacking the leader L protease and mutations in the receptor binding sequence RGD have also been proposed (3,16 …”

Section: Discussionmentioning

confidence: 99%

Attenuated Foot-and-Mouth Disease Virus RNA Carrying a Deletion in the 3′ Noncoding Region Can Elicit Immunity in Swine

Pulido

Sánchez‐Madrid

Borrego

et al. 2009

J Virol

View full text Add to dashboard Cite

We constructed foot-and-mouth disease virus (FMDV) mutants bearing independent deletions of the two stem-loop structures predicted in the 3 noncoding region of viral RNA, SL1 and SL2, respectively. Deletion of SL2 was lethal for viral infectivity in cultured cells, while deletion of SL1 resulted in viruses with slower growth kinetics and downregulated replication associated with impaired negative-strand RNA synthesis. With the aim of exploring the potential of an RNA-based vaccine against foot-and-mouth disease using attenuated viral genomes, full-length chimeric O1K/C-S8 RNAs were first inoculated into pigs. Our results show that FMDV viral transcripts could generate infectious virus and induce disease in swine. In contrast, RNAs carrying the ⌬SL1 mutation on an FMDV O1K genome were innocuous for pigs but elicited a specific immune response including both humoral and cellular responses. A single inoculation with 500 g of RNA was able to induce a neutralizing antibody response. This response could be further boosted by a second RNA injection. The presence of the ⌬SL1 mutation was confirmed in viruses isolated from serum samples of RNA-inoculated pigs or after transfection and five passages in cell culture. These findings suggest that deletion of SL1 might contribute to FMDV attenuation in swine and support the potential of RNA technology for the design of new FMDV vaccines. Foot-and-mouth disease virus (FMDV) is a member of thePicornaviridae family and the causative agent of an acute vesicular disease considered a major animal health problem worldwide, affecting pigs, ruminants, and other cloven-hoofed livestock (32, 53). The virus consists of a nonenveloped particle enclosing a single-stranded positive-sense RNA molecule of about 8.5 kb in length, with the viral protein VPg covalently linked to the 5Ј end and a poly(A) tract at the 3Ј end. The viral genome contains a single open reading frame flanked by two highly structured noncoding regions (NCRs) at their 5Ј and 3Ј termini, respectively (7). The 5Ј NCR, approximately 1,300 nucleotides in length, includes sequences required for the initiation of replication and translation, comprising the S fragment, a 360-nucleotide-long region predicted to form a large hairpin structure (23, 62), a poly(C) tract, multiple pseudoknots, the cis replication element (cre) (38), and the internal ribosome entry site (IRES) (37). The 3Ј NCR is a sequence of about 100 nucleotides predicted to be structured in two stem-loops, SL1 and SL2 (55). We have previously shown the strict requirement of the 3Ј NCR for FMDV infectivity and replication (52) and the stimulatory effect of this region on IRES-dependent translation, even in the absence of a poly(A) tail (36). Moreover, the 3Ј NCR interacts with the IRES and S regions located at the 5Ј end through direct RNA-RNA interactions (55). Cellular proteins binding to the 3Ј NCR have also been described (36,48). Some of these factors are also able to interact with the S region and undergo proteolytic cleavage upon FMDV infection (48). The pu...

show abstract

Section: Discussionmentioning

confidence: 99%

Attenuated Foot-and-Mouth Disease Virus RNA Carrying a Deletion in the 3′ Noncoding Region Can Elicit Immunity in Swine

Pulido

Sánchez‐Madrid

Borrego

et al. 2009

J Virol

View full text Add to dashboard Cite

show abstract

“…DNA vaccines can also induce cellular immunity and humoral immunity, but, unlike DNA vaccines, recombinant viruses are more stable, versatile for manufacture, storage, and have no limitation that DNA vaccines remains low relative efficacy, requiring multiple boosts with high doses. Several virus vectors were used as FMDV antigen delivery, such as fowlpox virus, pseudorabies virus, adenovirus (Qian et al 2004;Zheng et al 2006;Du et al 2007). Among the virus vectors, BHV-1 provides many unique advantages.…”

Section: Discussionmentioning

confidence: 99%

Construction of a recombinant BHV-1 expressing the VP1 gene of foot and mouth disease virus and its immunogenicity in a rabbit model

et al. 2009

View full text Add to dashboard Cite

Foot-and-mouth disease (FMD) and infectious bovine rhinotracheitis (IBR) are two important infectious diseases of cattle. Using bovine herpesvirus type 1 (BHV-1) as a gene delivery vector for development of live-viral vaccines has gained widespread interest. In this study, a recombinant BHV-1 was constructed by inserting the synthetic FMDV (O/China/99) VP1 gene in the the gE locus of BHV-1 genome under the control of immediately early gene promoter of human cytomegalovirus (phIE CMV) and bovine growth hormone polyadenylation (BGH polyA) signal. After homologous recombination and plaque purification, a recombinant virus named BHV-1/gE(-)/VP1 was acquired and identified. The immunogenicity was confirmed in a rabbit model by virus neutralization test and enzyme-linked immunosorbent assay (ELISA). The result indicated that the BHV-1/gE(-)/VP1 has the potential for being developed as a bivalent vaccine for FMD and IBR.

show abstract

“…Some researchers had an attempt to exploit recombinant vaccinia virus [32] fowlpox virus [33] and pseudorabies virus [34] expressing FMDV structural protein, non-structure protein or both of them, but swine and guinea pigs inoculated with multiple doses were partially protected because of their low level of expression. However, FMD vaccines are required to protect animals from FMDV challenge by a single dose of vaccine in the epidemic period.…”

Section: Live Vector Vaccinementioning

confidence: 99%

Research in advance for FMD Novel Vaccines

Zhang

Chen

et al. 2011

Virol J

View full text Add to dashboard Cite

Foot-and-Mouth Disease (FMD), as a major global animal disease, affects millions of animals worldwide and remains the main sanitary barrier to the international and national trade of animals and animal products. Inactivated vaccination is the most effective measure for prevention of FMD at present, but fail to induce long-term protection and content new requires for production of FMD vaccines. As a number of Researchers hope to obtain satisfactory novel vaccines by new bio-technology, novel vaccines have been studied for more than thirty years. Here reviews the latest research progress of new vaccines, summarizes some importance and raises several suggestions for the future of FMD vaccine.

show abstract

Construction and immunogenicity of a recombinant fowlpox virus containing the capsid and 3C protease coding regions of foot-and-mouth disease virus

Cited by 36 publications

References 29 publications

Attenuated Foot-and-Mouth Disease Virus RNA Carrying a Deletion in the 3′ Noncoding Region Can Elicit Immunity in Swine

Attenuated Foot-and-Mouth Disease Virus RNA Carrying a Deletion in the 3′ Noncoding Region Can Elicit Immunity in Swine

Construction of a recombinant BHV-1 expressing the VP1 gene of foot and mouth disease virus and its immunogenicity in a rabbit model

Research in advance for FMD Novel Vaccines

Contact Info

Product

Resources

About