Construction and Immunogenicity of Recombinant Vaccinia Virus Vaccine Against Japanese Encephalitis and Chikungunya Viruses Infection in Mice

Zhang, Ying; Han, Jin‐Hee; Jie, Jing; Liu, Hao; He, Zhonghu; Li, Zhaohui; Jin, Ningyi; Lu, Huijun

doi:10.1089/vbz.2020.2613

Cited by 6 publications

(3 citation statements)

References 43 publications

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“…All flow cytometry antibodies used were purchased from Biolegend (San Diego, CA, USA). The T lymphocyte proliferation detection experiment was operated in accordance with the method reported in the previous literature ( 20 ).…”

Section: Methodsmentioning

confidence: 99%

GII.P16-GII.2 Recombinant Norovirus VLPs Polarize Macrophages Into the M1 Phenotype for Th1 Immune Responses

Han

Fang

et al. 2021

Front. Immunol.

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Norovirus (NoV) is a zoonotic virus that causes diarrhea in humans and animals. Outbreaks in nosocomial settings occur annually worldwide, endangering public health and causing serious social and economic burdens. The latter quarter of 2016 witnessed the emergence of the GII.P16-GII.2 recombinant norovirus throughout Asia. This genotype exhibits strong infectivity and replication characteristics, proposing its potential to initiate a pandemic. There is no vaccine against GII.P16-GII.2 recombinant norovirus, so it is necessary to design a preventive vaccine. In this study, GII.P16-GII.2 type norovirus virus-like particles (VLPs) were constructed using the baculovirus expression system and used to conduct immunizations in mice. After immunization of mice, mice were induced to produce memory T cells and specific antibodies, indicating that the VLPs induced specific cellular and humoral immune responses. Further experiments were then initiated to understand the underlying mechanisms involved in antigen presentation. Towards this, we established co-cultures between dendritic cells (DCs) or macrophages (Mø) and naïve CD4+T cells and simulated the antigen presentation process by incubation with VLPs. Thereafter, we detected changes in cell surface molecules, cytokines and related proteins. The results indicated that VLPs effectively promoted the phenotypic maturation of Mø but not DCs, as indicated by significant changes in the expression of MHC-II, costimulatory factors and related cytokines in Mø. Moreover, we found VLPs caused Mø to polarize to the M1 type and release inflammatory cytokines, thereby inducing naïve CD4+ T cells to perform Th1 immune responses. Therefore, this study reveals the mechanism of antigen presentation involving GII.P16-GII.2 recombinant norovirus VLPs, providing a theoretical basis for both understanding responses to norovirus infection as well as opportunities for vaccine development.

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Section: Methodsmentioning

confidence: 99%

GII.P16-GII.2 Recombinant Norovirus VLPs Polarize Macrophages Into the M1 Phenotype for Th1 Immune Responses

Han

Fang

et al. 2021

Front. Immunol.

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“…Another VACV-derived vaccine, RABORAL V-RG, an antirabies vaccine expressing the rabies virus (RABV) glycoprotein gene (V-RG), has been used in Europe and North America to vaccinate foxes and raccoons [30]. Additionally, VACV-based vaccine candidates have been demonstrated to protect against emerging viral diseases, such as chikungunya virus (CHIKV) disease [41,42] and yellow fever [43] in preclinical animal models. The Sementis Copenhagen Vector (SCV) is a new multiplication-defective VACV-COP-derived vaccine vector with targeted deletion of D13L gene encoding D13 protein that is essential for viral assembly.…”

Section: Poxviral Vectors For Vaccine Applicationsmentioning

confidence: 99%

NF-κB as an Important Factor in Optimizing Poxvirus-Based Vaccines against Viral Infections

Struzik

Szulc-Dąbrowska

2020

Pathogens

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Poxviruses are large dsDNA viruses that are regarded as good candidates for vaccine vectors. Because the members of the Poxviridae family encode numerous immunomodulatory proteins in their genomes, it is necessary to carry out certain modifications in poxviral candidates for vaccine vectors to improve the vaccine. Currently, several poxvirus-based vaccines targeted at viral infections are under development. One of the important aspects of the influence of poxviruses on the immune system is that they encode a large array of inhibitors of the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB), which is the key element of both innate and adaptive immunity. Importantly, the NF-κB transcription factor induces the mechanisms associated with adaptive immunological memory involving the activation of effector and memory T cells upon vaccination. Since poxviruses encode various NF-κB inhibitor proteins, before the use of poxviral vaccine vectors, modifications that influence NF-κB activation and consequently affect the immunogenicity of the vaccine should be carried out. This review focuses on NF-κB as an essential factor in the optimization of poxviral vaccines against viral infections.

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“…The chimeric vaccine (ChimeriVax-JE) was generated by inserting the precursor membrane (prM) and envelope (Env) genes of SA-14-14-2 into Yellow fever (YF) 17D viral "backbone" to form a live-attenuated vaccine [7]. In addition, there are several vaccine candidates based on different strategies in preclinical research, including DNA vaccine [8][9][10], peptide and protein subunit vaccines [11][12][13], replication-defective vaccine [14], and virus vector vaccines [15].…”

Section: Introductionmentioning

confidence: 99%

A VLP-Based Vaccine Candidate Protects Mice against Japanese Encephalitis Virus Infection

Yang

Xiao²,

Wang³

et al. 2022

Vaccines

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Japanese encephalitis virus (JEV) is the leading cause of epidemic encephalitis in Asia, and vaccination is the most effective way to prevent JE. Although several licensed vaccines were widely used, there is still a demand for developing safer, cheaper, and more effective JE vaccines. In the current study, a virus-like particle (VLP) vaccine candidate containing the envelope structural protein of JEV expressed by the Pichia pastoris was assembled in vitro. It elicited a robust humoral and cellular immune response in mice model, conferring immunodeficient mice complete protection against lethal doses of JEV challenge. Furthermore, pigs immunized with VLP alone without adjuvant via intramuscular produced high neutralizing antibodies against JEV. Consequently, this study showed a new design of JEV subunit vaccine based on VLP strategy and demonstrated the potential for clinical application.

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Construction and Immunogenicity of Recombinant Vaccinia Virus Vaccine Against Japanese Encephalitis and Chikungunya Viruses Infection in Mice

Cited by 6 publications

References 43 publications

GII.P16-GII.2 Recombinant Norovirus VLPs Polarize Macrophages Into the M1 Phenotype for Th1 Immune Responses

GII.P16-GII.2 Recombinant Norovirus VLPs Polarize Macrophages Into the M1 Phenotype for Th1 Immune Responses

NF-κB as an Important Factor in Optimizing Poxvirus-Based Vaccines against Viral Infections

A VLP-Based Vaccine Candidate Protects Mice against Japanese Encephalitis Virus Infection

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