2020
DOI: 10.3390/v12030315
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Construction and Immunogenicity of Virus-Like Particles of Feline Parvovirus from the Tiger

Abstract: Feline panleukopenia, caused by feline parvovirus (FPV), is a highly infectious disease characterized by leucopenia and hemorrhagic gastroenteritis that severely affects the health of large wild Felidae. In this study, tiger FPV virus-like particles (VLPs) were developed using the baculovirus expression system. The VP2 gene from an infected Siberian tiger (Panthera tigris altaica) was used as the target gene. The key amino acids of this gene were the same as those of FPV, whereas the 101st amino acid was the s… Show more

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Cited by 8 publications
(8 citation statements)
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“…Nevertheless, the CPV-31 strain shows high similarity with strains isolated from Beijing and Italy, namely, CPV-L (99.78%) (GenBank accession: MG763189.1 ) in 2014 and CPV_IZSSI_2323_11 (99.56%) (GenBank accession: KX434458.1 ) in 2011, indicating that the virus is still circulating, although, those strains were discovered three decades ago. Meanwhile, Wang et al ( 35 ) and Jiao et al ( 36 ) isolated parvovirus from the captive Siberian tigers, which was closely related to racoon parvovirus, indicating the susceptibility of felids to parvovirus. In regard to variant, CPV-2a and CPV-2b have been isolated from cheetah and tiger while CPV-2c was isolated from leopard ( 37 , 38 ).…”
Section: Discussionmentioning
confidence: 99%
“…Nevertheless, the CPV-31 strain shows high similarity with strains isolated from Beijing and Italy, namely, CPV-L (99.78%) (GenBank accession: MG763189.1 ) in 2014 and CPV_IZSSI_2323_11 (99.56%) (GenBank accession: KX434458.1 ) in 2011, indicating that the virus is still circulating, although, those strains were discovered three decades ago. Meanwhile, Wang et al ( 35 ) and Jiao et al ( 36 ) isolated parvovirus from the captive Siberian tigers, which was closely related to racoon parvovirus, indicating the susceptibility of felids to parvovirus. In regard to variant, CPV-2a and CPV-2b have been isolated from cheetah and tiger while CPV-2c was isolated from leopard ( 37 , 38 ).…”
Section: Discussionmentioning
confidence: 99%
“…There have been several studies on the expression of either recombinant full-length or partial VP2 proteins of CPV, such as in mammalian cells [ 10 , 19 ], insect cells [ 20 , 21 ], and E. coli system [ 7 , 9 , 22 ]. The recombinant full-length and 35 kDa fragment VP2 proteins in the present study were insoluble, as observed in other studies that used E. coli as the VP2 expression host [ 9 , 22 ].…”
Section: Discussionmentioning
confidence: 99%
“…Proteins were transferred utilizing the Trans-Blot ® Turbo TM Transfer System (Bio-Rad Laboratories, Hercules, CA, United States) from gels onto PVDF membranes. Membranes were blocked with PBS, 5% (w/v) skim milk, and 0.5% (v/v) Tween 20 for 2 h at room temperature and incubated with mouse monoclonal antibody recognizing CPV VP2 protein (Abcam, Cambridge, United Kingdom) ( 9 , 21 ) at 4°C overnight. Following multiple rinses with PBS-0.05% Tween 20, the membranes were then incubated at 37°C for 1 h with HRP-conjugated goat anti-mouse IgG (Abcam, Cambridge, United Kingdom).…”
Section: Methodsmentioning
confidence: 99%