Construction and &lt;i&gt;in vitro&lt;/i&gt; characterization of three-dimensional silk fibroinchitosan scaffolds

Tong, S. Y.; Xu, Di; Liu, Zi-mei; Wang, Xukai

doi:10.4012/dmj.2014-251

Cited by 12 publications

(9 citation statements)

References 26 publications

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“…The compression modulus was defi ned as the slope of the initial linear part of the stress-strain curve. From 1% strain, a straight line was drawn parallel to the initial linear section of the curve, and the ordinate of the intersection with the stressstrain curve was taken as the compression strength 23) . The initial quality of each sample was recorded as m0, then the samples were placed in a 24-well culture plate and sterilized using a Co-60SQ(H)630 (Shanqiang Nuclear Radiation Engineering Technology, Beijing, China).…”

Section: Preparation Of Bfgf/nhap/col Scaff Oldsmentioning

confidence: 99%

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Withdrawal: Synthesis of a Novel bFGF/nHAP/COL Bone Tissue Engineering Scaffold for Mandibular Defect Regeneration in a Rabbit Model

Yue

Tan

Zhao

et al. 2018

J. Hard Tissue Biology.

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In bone tissue engineering, scaffold fabrication and biocompatibility are crucial concerns. Many scaffold materials have been explored, among which nanometer hydroxyapatite (nHAP) and collagen (COL) are commonly used. Additionally, growth factors can be used to modify scaff olds. In this study, lyophilization technology was used to build a scaff old comprising basic fi broblast growth factor (bFGF), nHAP and COL for the fi rst time. The resulting scaff old was characterized. bFGF release from the scaff old was assessed by ELISA. Bone marrow mesenchymal stem cells (BMSCs) were prepared and seeded onto the scaff old to test in vitro biological compatibility. A scanning electron microscope was used to observe the scaff old and evaluate BMSC morphology, and the cells were counted to detect early cell adhesion. Cell proliferation and activity were assessed by a cell counting kit-8 assay and measurement of alkaline phosphatase activity, respectively. Bilateral mandibular defects were prepared in 12 New Zealand rabbits and repaired using scaff olds. The rabbits were divided into four groups: a group treated with allogeneic BMSC-seeded bFGF/nHAP/COL scaff old, a group treated with allogeneic BMSC-seeded nHAP/COL scaff old, a group treated with nHAP/COL scaff old alone, and an untreated control group. After 12 weeks, three-dimensional computerized tomography examination, computerized tomography value measurement, gross observation and hematoxylin and eosin stain staining were conducted. SPSS17.0 software was used for data analysis. The gross morphology conformed to the characteristics of a tissue engineering scaff old. The bFGF/nHAP/COL scaff old promoted BMSC adhesion, proliferation and diff erentiation and hence promoted good bone formation, without exhibiting biological toxicity. Our fi ndings show that the bFGF/nHAP/COL scaff old has good physical properties and biocompatibility in vitro, and can be used to promote osteogenesis after in vivo implantation.

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Section: Preparation Of Bfgf/nhap/col Scaff Oldsmentioning

confidence: 99%

“…The scaff old was dried in an oven (Yuejin Medical Optical Instruments, Shanghai, China), and the dry weight was recorded as S2. The water absorption capacity was calculated as (S2 / S1 − 1) × 100% 23,24) . The compression strength and modulus were obtained by calculation of a stress-strain curve.…”

Section: Preparation Of Bfgf/nhap/col Scaff Oldsmentioning

confidence: 99%

Withdrawal: Synthesis of a Novel bFGF/nHAP/COL Bone Tissue Engineering Scaffold for Mandibular Defect Regeneration in a Rabbit Model

Yue

Tan

Zhao

et al. 2018

J. Hard Tissue Biology.

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“…Optimum mechanical properties (pore size, tensile strength, etc.) and biocompatibility of the constructs are potential features to be considered for cell colonization and organization [ 17 ]. The presented work describes fabrication and characterization of scaffold for bone tissue engineering utilizing combination of beneficial properties of materials proposed for the same.…”

Section: Discussionmentioning

confidence: 99%

Bone Morphogenic Protein-2 (rhBMP2)-Loaded Silk Fibroin Scaffolds to Enhance the Osteoinductivity in Bone Tissue Engineering

Sun

et al. 2017

Nanoscale Res Lett

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There is an increasing demand for formulations of silk fibroin (SF) scaffolds in biomedical applications. SF was crosslinked via glutaraldehyde with osteoinductive recombinant human bone morphogenic protein-2 (rhBMP2) of different ratios viz. (i) 3% SF with no rhBMP2 (SF), (ii) 3% SF with equal amount of rhBMP2 (SF+BMP2), and (iii) 12% SF with 3% of rhBMP2 (4SF+BMP2), and these solutions were used in electrospinning-based fabrication of nanoscaffolds for evaluating increased osteoinductive potential of SF scaffolds with rhBMP2. Stress–strain relationship suggested there is no loss in mechanical strength of fibers with addition of rhBMP2, and mechanical strength of scaffold was improved with increase in concentration of SF. rhBMP2 association increased the water retention capacity of scaffold as evident from swelling studies. Viability of hMSCs was found to be higher in conjugated scaffolds, and scaffolds do not exhibit any cytotoxicity towards guest cells. Cells were found to have higher alkaline phosphatase activity in conjugated scaffolds under in vitro and in vivo conditions which establishes the increased osteoinductivity of the novel construct. The scaffolds were found to be effective for in vivo bone formation as well.

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“…Scaffold morphology was observed by scanning electron microscopy (SEM; S4800, Hitachi, Japan) operated at 15 kV 18) . Scaffold samples for SEM examination were frozen in liquid nitrogen and fractured using a razor blade.…”

Section: Scaffold Morphologymentioning

confidence: 99%

Characterization of a Porous BMP-6-Loaded Composite Scaffold for Bone Regeneration in Rat Calvarial Bone Defects

Zhang

Yang

et al. 2019

J. Hard Tissue Biology.

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Biocompatible scaffolding materials play an important role in bone tissue engineering. This study sought to assess the biocompatibility and osteoinductivity of a chitosan (CS)/collagenⅠ(ColI)/multi-walled carbon nanotube (MWCNT) composite scaffold blended with bone morphogenetic protein-6 (BMP-6) and seeded with bone marrow mesenchymal stem cells (BMMSCs). The composite was fabricated using varying quantities of MWCNT to determine the appropriate combination, and combined with BMP-6 by blending and vacuum freeze-drying. Scanning electron microscopy (SEM) was used to observe the morphology of the CS/ColI/MWCNT (CCM) scaffolds. The porosity and mechanical properties of the composite scaffolds were measured. Scaffolds were seeded with BMMSCs in vitro and analyzed using SEM, CCK-8 assay and alkaline phosphatase (ALP) activity. To assess the in vivo utility of the composite scaffolds, critical-sized calvarial bone defects were prepared in 15 Sprague-Dawley rats and implanted with pre-seeded scaffolds (the CCM scaffold+BMMSCs group and the BMP-6/CCM scaffold+BMMSCs group) or left untreated (control). Three-dimensional computerized tomography and hematoxylin-eosin (HE) staining were used to evaluate bone formation at 8 weeks postoperatively. We found that higher concentrations of MWCNT decreased the porosity of the scaffold but increased its mechanical strength. In vitro, we found that BMP-6/CCM scaffolds provided the best conditions for BMMSCs proliferation and osteogenic differentiation. Similarly, BMP-6/CCM scaffolds seeded with BMMSCs could more effectively induce new bone formation in critical-sized calvarial bone defects compared with the other three groups. The BMP-6/CCM scaffold possesses good biocompatibility and induces bone formation in vitro and in vivo.

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Construction and <i>in vitro</i> characterization of three-dimensional silk fibroinchitosan scaffolds

Cited by 12 publications

References 26 publications

Withdrawal: Synthesis of a Novel bFGF/nHAP/COL Bone Tissue Engineering Scaffold for Mandibular Defect Regeneration in a Rabbit Model

Withdrawal: Synthesis of a Novel bFGF/nHAP/COL Bone Tissue Engineering Scaffold for Mandibular Defect Regeneration in a Rabbit Model

Bone Morphogenic Protein-2 (rhBMP2)-Loaded Silk Fibroin Scaffolds to Enhance the Osteoinductivity in Bone Tissue Engineering

Characterization of a Porous BMP-6-Loaded Composite Scaffold for Bone Regeneration in Rat Calvarial Bone Defects

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