Construction and nucleotide sequence analysis of an infectious DNA clone of the autonomous parvovirus, mink enteritis virus

Abstract

We have cloned the replicative form (RF-) DNA of mink enteritis virus (MEV), constructed an infectious recombinant plasmid containing MEV DNA and determined the nucleotide sequence of the clbned MEV DNA. RF-DNAs were detected and infectious virus was generated when the recombinant plasmid containing the entire MEV genome was introduced into feline kidney cell cultures. The MEV genome was 5094 nucleotides (nt) in length; the 3' end of the virion strand contained a 205 nt palindromic sequence and the 5' end a 62 nt palindromic sequence that could assume Y-and U-shaped configurations, respectively. The 5' end of the virion strand had a direct repeat of 61 nt at the carboxyl terminus of the capsid protein gene. The organization of the MEV genome is similar to those of canine parvovirus (CPV) and feline panleukopenia virus (FPLV); there are two large open reading frames (ORFs), one in the 3' half and the other in the 5' half of the genome, with coding capacities of 668 and 722 amino acid residues, respectively. Both are in the same reading frame and no significant ORFs are apparent in the virion strand (negative-sense strand). Possible functional promoter motifs are located at map unit (m.u.) 4-5 and m.u. 40, and a possible functional poly(A) signal is located at m.u. 96. The nucleotide and amino acid sequence homology with CPV and FPLV is greater than 98 %, consistent with the hypothesis that MEV and CPV are host-range variants of FPLV.

Mapping of determinants of the host range for canine cells in the genome of canine parvovirus using canine parvovirus/mink enteritis virus chimeric viruses

“…Recombinants were constructed from pMEV, an infectious plasmid clone of the MEVAbashiri strain (Kariatsumari et al, 1991), and pCPVY 1, an infectious plasmid clone of the CPV-Y1 strain (Horiuchi & Shinagawa, 1993), according to standard procedures (Sambrook et aL, 1989). The 3' and the 5' ends of the CPV genome are 44 bp shorter and 63 bp longer than those of the MEV genome, respectively, and nucleotides (nt) 4593 to 4633 of the MEV sequence are not found in CPV (depicted in Fig.…”

“…structural protein (NS) in the feline parvovirus subgroup, whereas the right-hand ORF encodes two capsid proteins, VP1 and VP2, which are translated from alternatively spliced RNA. Partial and almost whole nucleotide sequences of CPV, FPLV and MEV have been determined (Carlson et al, 1985;Parrish et al, 1988a;Reed et al, 1988;Martyn et al, 1990;Kariatsumari et al, 1991). They have more than 98% identity in DNA and predicted amino acid sequences (Kariatsumari et al, 1991); however, CPV and FPLV/MEV are distinguishable in some biological properties such as the pH dependence of haemagglutination (HA; Carmichael et al, 1980) and their host cell specificity in vitro and in vivo.…”

“…Partial and almost whole nucleotide sequences of CPV, FPLV and MEV have been determined (Carlson et al, 1985;Parrish et al, 1988a;Reed et al, 1988;Martyn et al, 1990;Kariatsumari et al, 1991). They have more than 98% identity in DNA and predicted amino acid sequences (Kariatsumari et al, 1991); however, CPV and FPLV/MEV are distinguishable in some biological properties such as the pH dependence of haemagglutination (HA; Carmichael et al, 1980) and their host cell specificity in vitro and in vivo. CPV isolates can replicate in canine and feline cell lines; however, FPLV and MEV do not replicate or replicate only poorly in canine cell lines such as A72 canine fibroma, MDCK and Cf2Th (Tratschin et al, 1982;Mochizuki & Hashimoto, 1986;Parrish et al, 1988a;Horiuchi et al, 1992;Truyen & Parrish, 1992).…”

Production of mink enteritis parvovirus empty capsids by expression in a baculovirus vector system: a recombinant vaccine for mink enteritis parvovirus in mink

“…The MEV virion consists of a linear negative-sense single-stranded DNA of approximately 5 kb (Kariatsumari et al, 1991) encapsidated by three structural proteins, VP-1 (77"5K), VP-2 (63.5K) and VP-3 (63K) (Carman & Povey, 1983). Based on the transcription data from minute virus of mice (Jongeneel et al, 1986), t Present address: National Veterinary Laboratory, Btilowsvej 27, DK-1790 Copenhagen V, Denmark.…”

Construction of an infectious DNA clone of the Y 1 strain of canine parvovirus and characterization of the virus derived from the clone

“…CPV is classified as the feline parvovirus subgroup of genus Parvovirus, within the family Parvoviridae [28]. Feline panleukopenia virus (FPLV) and mink enteritis virus (MEV) are also members of this group, and have more than 98% nucleotide sequence identity to CPV [11], although CPV is distinguishable from FPLV and MEV by some biological features such as host cell tropism [8,16,30,32] and hemagglutination dependence on pH [4,25]. The virion contains a linear, single-stranded (ssDNA) genome of about 5 kilobases (kb) in length.…”