Construction and optimization of a photo−enzyme coupled system for sustainable CO2 conversion to methanol

Liao, Qiyong; Guo, Mengya; Mao, Menglei; Gao, Rui; Meng, Zihui; Fan, Xiaodong; Liu, Wenfang

doi:10.1016/j.procbio.2023.03.011

Cited by 5 publications

(5 citation statements)

References 55 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Here, we chose to further couple EPCS to cell to form a semiconductor–enzyme–cell hybrid system, in which native or synthetic methylotrophic cells intake methanol and assimilate it to biomass. The semiconductor–enzyme–cell hybrid system can produce methanol at 4.07 mg/(L·h), on par with reported rates from photocatalytic systems containing mediators or photoelectrochemical cells ranging from 2.2 to 8.2 mg/(L·h). ,, The semiconductor–enzyme–cell hybrid system transports methanol, a more stable and less toxic species across the membrane while direct photosensitization of cells deals with electrons. The current g-C 3 N 4 –FDH–FaldDH–ADH enzyme cascade–P.…”

Section: Resultsmentioning

confidence: 76%

“…The semiconductor− enzyme−cell hybrid system can produce methanol at 4.07 mg/ (L•h), on par with reported rates from photocatalytic systems containing mediators or photoelectrochemical cells ranging from 2.2 to 8.2 mg/(L•h). 30,32,52 The semiconductor− enzyme−cell hybrid system transports methanol, a more stable and less toxic species across the membrane while direct photosensitization of cells deals with electrons. The current g-C 3 N 4 −FDH−FaldDH−ADH enzyme cascade−P.…”

Section: Co-immoblization Of Cascade Enzymes Allowsmentioning

confidence: 99%

See 1 more Smart Citation

Light-Driven CO₂ Reduction with a Surface-Displayed Enzyme Cascade–C₃N₄ Hybrid

Sheng,

Guo,

Guo

et al. 2023

ACS Synth. Biol.

View full text Add to dashboard Cite

Efficient and cost-effective conversion of CO 2 to biomass holds the potential to address the climate crisis. Lightdriven CO 2 conversion can be realized by combining inorganic semiconductors with enzymes or cells. However, designing enzyme cascades for converting CO 2 to multicarbon compounds is challenging, and inorganic semiconductors often possess cytotoxicity. Therefore, there is a critical need for a straightforward semiconductor biohybrid system for CO 2 conversion. Here, we used a visible-light-responsive and biocompatible C 3 N 4 porous nanosheet, decorated with formate dehydrogenase, formaldehyde dehydrogenase, and alcohol dehydrogenase to establish an enzymephotocoupled catalytic system, which showed a remarkable CO 2to-methanol conversion efficiency with an apparent quantum efficiency of 2.48% in the absence of externally added electron mediator. To further enable the in situ transformation of methanol into biomass, the enzymes were displayed on the surface of Komagataella phaffii, which was further coupled with C 3 N 4 to create an organic semiconductor−enzyme−cell hybrid system. Methanol was produced through enzyme-photocoupled CO 2 reduction, achieving a rate of 4.07 mg/(L•h), comparable with reported rates from photocatalytic systems employing mediators or photoelectrochemical cells. The produced methanol can subsequently be transported into the cell and converted into biomass. This work presents a sustainable, environmentally friendly, and cost-effective enzyme-photocoupled biocatalytic system for efficient solardriven conversion of CO 2 within a microbial cell.

show abstract

Section: Resultsmentioning

confidence: 76%

Section: Co-immoblization Of Cascade Enzymes Allowsmentioning

confidence: 99%

Light-Driven CO₂ Reduction with a Surface-Displayed Enzyme Cascade–C₃N₄ Hybrid

Sheng,

Guo,

Guo

et al. 2023

ACS Synth. Biol.

View full text Add to dashboard Cite

show abstract

“…Liu et al have also reported the three co-immobilized dehydrogenases on hollow fiber membranes but using commercial pristine TiO 2 as simple UV/TiO 2 photocatalytic and clean H 2 O as an electron donor making the process green and sustainable. 85 With the in situ regeneration of NADH, methanol yield could reach 38.6% after 5 h, which was 3.81 times that of the single enzyme-catalyzed system.…”

Section: Capture and Valorisation Of Co2 For Chemicalsmentioning

confidence: 91%

From green to circular chemistry paved by biocatalysis

Lozano¹,

García‐Verdugo²

2023

Green Chem.

View full text Add to dashboard Cite

show abstract

“…Various photosensitizers have also been used. Rh(III) and Ru(III) bipyridyl complexes are well known for their photosensitivity; consequently, their use has been extended [ 264 , 265 , 266 , 267 , 268 , 269 ]. Guo et al employed Cp*Rh(bpy)(H 2 O)] 2+ (Cp = cyclopentadienyl; bpy = 2,2′-bipyridyl) as a synthesizer in a system where FDH and FalDH were immobilized on polyethylene membranes doped with the widely used semiconductor TiO 2 [ 270 ].…”

Section: Biotechnological Aspects Of Co 2 Reductionmentioning

confidence: 99%

“…Scheme of the photochemical reduction of CO 2 using FDH.Various photosensitizers have also been used. Rh(III) and Ru(III) bipyridyl complexes are well known for their photosensitivity; consequently, their use has been extended[264][265][266][267][268][269].Guo et al employed Cp*Rh(bpy)(H 2 O)] 2+ (Cp = cyclopentadienyl; bpy = 2,2 -bipyridyl) as a synthesizer in a system where FDH and FalDH were immobilized on polyethylene membranes doped with the widely used semiconductor TiO 2[270]. A comparison between the results using water or EDTA as a sacrificial agent and as a function of pH was described, the latter being much more efficient for formaldehyde production.…”

mentioning

confidence: 99%

Direct Biocatalytic Processes for CO2 Capture as a Green Tool to Produce Value-Added Chemicals

et al. 2023

View full text Add to dashboard Cite

Direct biocatalytic processes for CO2 capture and transformation in value-added chemicals may be considered a useful tool for reducing the concentration of this greenhouse gas in the atmosphere. Among the other enzymes, carbonic anhydrase (CA) and formate dehydrogenase (FDH) are two key biocatalysts suitable for this challenge, facilitating the uptake of carbon dioxide from the atmosphere in complementary ways. Carbonic anhydrases accelerate CO2 uptake by promoting its solubility in water in the form of hydrogen carbonate as the first step in converting the gas into a species widely used in carbon capture storage and its utilization processes (CCSU), particularly in carbonation and mineralization methods. On the other hand, formate dehydrogenases represent the biocatalytic machinery evolved by certain organisms to convert CO2 into enriched, reduced, and easily transportable hydrogen species, such as formic acid, via enzymatic cascade systems that obtain energy from chemical species, electrochemical sources, or light. Formic acid is the basis for fixing C1-carbon species to other, more reduced molecules. In this review, the state-of-the-art of both methods of CO2 uptake is assessed, highlighting the biotechnological approaches that have been developed using both enzymes.

show abstract

Construction and optimization of a photo−enzyme coupled system for sustainable CO2 conversion to methanol

Cited by 5 publications

References 55 publications

Light-Driven CO₂ Reduction with a Surface-Displayed Enzyme Cascade–C₃N₄ Hybrid

Light-Driven CO₂ Reduction with a Surface-Displayed Enzyme Cascade–C₃N₄ Hybrid

From green to circular chemistry paved by biocatalysis

Direct Biocatalytic Processes for CO2 Capture as a Green Tool to Produce Value-Added Chemicals

Contact Info

Product

Resources

About

Construction and optimization of a photo−enzyme coupled system for sustainable CO2 conversion to methanol

Cited by 5 publications

References 55 publications

Light-Driven CO2 Reduction with a Surface-Displayed Enzyme Cascade–C3N4 Hybrid

Light-Driven CO2 Reduction with a Surface-Displayed Enzyme Cascade–C3N4 Hybrid

From green to circular chemistry paved by biocatalysis

Direct Biocatalytic Processes for CO2 Capture as a Green Tool to Produce Value-Added Chemicals

Contact Info

Product

Resources

About

Light-Driven CO₂ Reduction with a Surface-Displayed Enzyme Cascade–C₃N₄ Hybrid

Light-Driven CO₂ Reduction with a Surface-Displayed Enzyme Cascade–C₃N₄ Hybrid