Construction from Destruction: Hydrogel Formation from Triggered Depolymerization-Based Release of an Enzymatic Catalyst

Kumar, Vikash; Harris, Justin T.; Ribbe, Alexander E.; Franc, Marina; Bae, Youngju; McNeil, Anne J.; Thayumanavan, S.

doi:10.1021/acsmacrolett.0c00023

Cited by 14 publications

(9 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Polymeric hydrogels are beneficial for biomedical applications, − such as tissue engineering and drug/gene delivery systems, since the hydrolyzed microenvironments provide cells/tissues with adjustable physicochemical properties and structure similar to the extracellular matrix in a swollen state. Hydrogels are generally prepared by cross-linked polymers to form highly interpenetrating networks .…”

mentioning

confidence: 99%

Synthesis and Evaluation of Cytocompatible Alkyne-Containing Poly(β-amino ester)-Based Hydrogels Functionalized via Click Reaction

Yao

Gong

et al. 2020

ACS Macro Lett.

View full text Add to dashboard Cite

Although poly(β-amino esters) (PAEs) have been widely applied in nonviral gene transfection, drug delivery systems, and regenerative medicine, the multifunctional modification of PAEs and bio-orthogonal strategies of PAE-based hydrogel functionalization is still a challenge. Herein, a strategy of poly(β-amino ester)-based hydrogel functionalization was developed via bio-orthogonal reactions in this study. Acrylate-terminated poly(β-amino esters) containing alkyne groups were synthesized by Michael addition reaction. Alkyne groups on poly(β-amino esters) could conjugate bioactive molecules with azide of K(N3)RGD via copper-catalyzed azide–alkyne cycloaddition, and terminal acrylate groups could in situ polymerize to prepare a hydrogel. A biomimetic peptide K(N3)RGD functionalized hydrogel was prepared by polymerization of acrylate-terminated poly(β-amino esters) containing conjugated peptide and polyethylene glycol diacrylate (PEGDA). The storage modulus and mechanical properties exhibited an increased trend with the increased concentration; nevertheless, swelling ratio and surface wetting properties demonstrated a decreased tendency by increased concentrations. Cell proliferation and live/dead staining showed that Schwann cells plated on the hydrogel with an elastic modulus of 25.39 KPa are more suitable for proliferation and function exertion of Schwann cells compared with that of 42.11 and 57.86 KPa, and KRGD-conjugated hydrogel could increase the elongation of Schwann cells relative to nonconjugated hydrogels. This azide–alkyne strategy may be a promising candidate for hydrogel functionalization in tissue engineering and other biomedical applications.

show abstract

mentioning

confidence: 99%

Synthesis and Evaluation of Cytocompatible Alkyne-Containing Poly(β-amino ester)-Based Hydrogels Functionalized via Click Reaction

Yao

Gong

et al. 2020

ACS Macro Lett.

View full text Add to dashboard Cite

show abstract

“…As shown in Figures 2E and 2F, the OSN gel with Tetra PEG of 20 kDa showed outstanding compressive strength (1.7 MPa) and compressive modulus (30 kPa). The mechanical properties of OSN gels were superior to those of recently reported Tetra PEG hydrogels under the same solid content, [ 25‐27 ] probably due to the high chemical reactivity of the OPA for the ternary condensation.…”

Section: Resultsmentioning

confidence: 65%

A Versatile Crosslinking Strategy on Facile Fabrication of Fluorescent Hydrogels via o‐Phthalaldehyde Ternary Condensation

Cao

Wang

et al. 2021

Chin. J. Chem.

View full text Add to dashboard Cite

Rapid gelation | Polymerization | Fluorescence | Gels | AldehydesIt is of great significance to develop rapid crosslinking strategies to enrich the functions of hydrogels and simplify the preparation process of hydrogels. Herein, we applied the ternary condensation reaction of o-phthalaldehyde (OPA) with thiol and amino moieties to construct hydrogel networks with fast gelation rate, excellent mechanical strength, and favorable stability. The obtained hydrogels exhibited rapid gelation transition even at a low solid content. The ternary condensation was traced in the gelation process and demonstrated as a pseudo-first-order reaction irrespective of the gelation threshold. Interestingly, the crosslinking sites of these hydrogels were constructed with isoindole rings, endowing the materials with fluorescent functions without an extra chemical grafting process. The OPA ternary condensation reaction was a simple and universal strategy towards fabrication of natural or synthetic polymer gels from polysaccharides, proteins, and synthetic polymers, which is expected to have potential biomedical applications.

show abstract

“…Enzymatic catalysis is an environmentally friendly strategy to catalyze radical polymerization to enable the self-setting of hydrogels without external stimuli [ 27 , 28 ]. Enzymes catalyze metabolic reactions and generate biomacromolecules in living cells [ 29 , 30 ].…”

Section: Introductionmentioning

confidence: 99%

Mussel-inspired nanozyme catalyzed conductive and self-setting hydrogel for adhesive and antibacterial bioelectronics

Jia

Hou

et al. 2021

Bioactive Materials

160

120

View full text Add to dashboard Cite

Adhesive hydrogels have broad applications ranging from tissue engineering to bioelectronics; however, fabricating adhesive hydrogels with multiple functions remains a challenge. In this study, a mussel-inspired tannic acid chelated-Ag (TA-Ag) nanozyme with peroxidase (POD)-like activity was designed by the in situ reduction of ultrasmall Ag nanoparticles (NPs) with TA. The ultrasmall TA-Ag nanozyme exhibited high catalytic activity to induce hydrogel self-setting without external aid. The nanozyme retained abundant phenolic hydroxyl groups and maintained the dynamic redox balance of phenol-quinone, providing the hydrogels with long-term and repeatable adhesiveness, similar to the adhesion of mussels. The phenolic hydroxyl groups also afforded uniform distribution of the nanozyme in the hydrogel network, thereby improving its mechanical properties and conductivity. Furthermore, the nanozyme endowed the hydrogel with antibacterial activity through synergistic effects of the reactive oxygen species generated via POD-like catalytic reactions and the intrinsic bactericidal activity of Ag. Owing to these advantages, the ultrasmall TA-Ag nanozyme-catalyzed hydrogel could be effectively used as an adhesive, antibacterial, and implantable bioelectrode to detect bio-signals, and as a wound dressing to accelerate tissue regeneration while preventing infection. Therefore, this study provides a promising approach for the fabrication of adhesive hydrogel bioelectronics with multiple functions via mussel-inspired nanozyme catalysis.

show abstract

Construction from Destruction: Hydrogel Formation from Triggered Depolymerization-Based Release of an Enzymatic Catalyst

Cited by 14 publications

References 28 publications

Synthesis and Evaluation of Cytocompatible Alkyne-Containing Poly(β-amino ester)-Based Hydrogels Functionalized via Click Reaction

Synthesis and Evaluation of Cytocompatible Alkyne-Containing Poly(β-amino ester)-Based Hydrogels Functionalized via Click Reaction

A Versatile Crosslinking Strategy on Facile Fabrication of Fluorescent Hydrogels via o‐Phthalaldehyde Ternary Condensation

Mussel-inspired nanozyme catalyzed conductive and self-setting hydrogel for adhesive and antibacterial bioelectronics

Contact Info

Product

Resources

About