Construction, Identification and Analysis of the Interaction Network of African Swine Fever Virus MGF360-9L with Host Proteins

Abstract

African swine fever virus (ASFV) is prevalent in many countries and is a contagious and lethal virus that infects pigs, posing a threat to the global pig industry and public health. The interaction between the virus and the host is key to unlocking the mystery behind viral pathogenesis. A comprehensive understanding of the viral and host protein interaction may provide clues for developing new antiviral strategies. Here, we show a network of ASFV MGF360-9L protein interactions in porcine kidney (PK-15) cells. Overall, 268 proteins that interact with MGF360-9L are identified using immunoprecipitation and liquid chromatography–mass spectrometry (LC-MS). Accordingly, gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were conducted, and the protein–protein interaction (PPI) network was created. It was speculated that the cellular proteins interacting with MGF360-9L are involved in protein binding, metabolism, and the innate immune response. Proteasome subunit alpha type (PSMA3), 26S protease regulatory subunit 4 (PSMC1), autophagy and beclin 1 regulator 1 (AMBRA1), and DEAD-box helicase 20 (DDX20) could interact with MGF360-9L in vitro. PSMA3 and PSMC1 overexpression significantly promoted ASFV replication, and MGF360-9L maintained the transcriptional level of PSMA3 and PSMC1. Here, we show the interaction between ASFV MGF360-9L and cellular proteins and elucidate the virus–host interaction network, which effectively provides useful protein-related information that can enable further study of the potential mechanism and pathogenesis of ASFV infection.

Proteins in pregnant swine serum promote the African swine fever virus replication: an iTRAQ-based quantitative proteomic analysis

“…The sequences used were as follows: ASFV- P72 -R: 5′-CTGCTCATGGTATCAATCTTATCGA-3′; ASFV- P72 -F: 5′-GATACCACAAGATCAGCCGT-3′; and TaqMan: 5′-CCACGGGAGGAATACCAACCCAGTG-3′. The ASFV genome copy number was calculated using the standard curve [ 22 ].…”

African Swine Fever Virus MGF110-7L Induces Host Cell Translation Suppression and Stress Granule Formation by Activating the PERK/PKR-eIF2α Pathway

“…Then, the protein bands corresponding to the empty vector and MGF110-7L-Flag pulldown lanes that differed in staining intensity were excised from the gel and subsequently subjected to in-gel tryptic digestion to extract peptides for further LC-MS/MS analysis. LC-MS/MS analysis was performed as described previously ( 54 ). After the monoisotopic mass lists for each protonated peptide were obtained, protein identification and quantification were carried out using Mascot 2.3.02 (MatrixScience, London, UK) against the Swiss-Prot Homo sapiens sequence database.…”

Cell Lines for the Development of African Swine Fever Virus Vaccine Candidates: An Update

“…Recently, Oh et al (2021) compared MA-104 to the renal-derived swine macrophages; they observed that MA-104 is less able to isolate the field virus, although both wild-type (MW039157) and cell-adapted (MW287337) ASFV genotype II strains grew efficiently in this continuous cell line [ 50 ]. This cell line was also efficiently used to study the interaction network of ASFV with host proteins [ 99 ] and to determine the mechanisms used by ASFV to evade the host innate immune responses [ 100 ].…”