Construction of a Artificial Glutathione Peroxidase with Temperature‐Dependent Activity Based on a Supramolecular Graft Copolymer

Abstract

A supramolecular artificial glutathione peroxidase (PNIPAM-CD-g-Te) was prepared based on a supramolecular graft copolymer. PNIPAM-CD-g-Te was constructed by supramolecular host-guest self-assembly. Significantly, PNIPAM-CD-g-Te displayed noticeable temperature-dependent catalytic activity and typical saturation kinetics behavior. It was also proved that the change in the self-assembled structure of PNIPAM-CD-g-Te during the temperature-dependent process played a significant role in the temperature-dependent catalytic behavior. The construction of PNIPAM-CD-g-Te based on supramolecular graft copolymer endows artificial GPx with temperature-dependent catalytic ability, enriched catalytic centers, and homogeneously distributed catalytic centers. This work bodes well for the development of other biologically related host-guest supramolecular biomaterials.

Facile Construction of Microgel based Biomimetic Glutathione Peroxidase with Temperature Responsive Catalytic Activity

“…b The supramolecualr microgel artificial GPx constructed in our previous report . c The supramolecular GPx mimic based on a supramolecular graft copolymer . d Tthe microgel artificial GPx (Microgel) constructed in our previous report .…”

“…In order to increase the catalytic activity of biomimetic GPx and elucidate the synergistic catalytic mechanism of natural GPx, our groups have constructed some efficient biomimetic GPxs using block polymers, graft polymers, supramolecular polymers and microgels as scaffolds. Recently, the smart biomimetic GPx with a intelligently regulated catalytic activity has been developed to respond the need of intelligent regulation for ROS concentration .…”

One-pot synthesis of biomimetic glutathione peroxidase with temperature responsive catalytic behaviors

“…b The supramolecular GPx mimic based on asupramolecular gra copolymer. 29 c The microgel articial GPx (Microgel) constructed in our previous report. 30 d The supramolecualr microgel articial GPx constructed in our previous report.…”

“…6,7,20,21 Through the biomimetic simulation of GPx catalytic center in natural GPx, researchers have prepared a series of biomimetic GPx. Generally, the scaffold materials for preparing biomimetic GPx mainly include small organic molecules, 12,13,[20][21][22] imprinted proteins, 8,11 genetically engineered proteins, 14,15,23 supramolecular polymers, 17,19,24,25 block polymers, [26][27][28][29] microgels, [30][31][32] and organic-inorganic hybrid materials. [33][34][35][36] Compared with natural GPx, these biomimetic GPx were endowed with obvious advantages, such as high preparation yield, strong structural stability and good industrialization prospect.…”

“…It is of great signicance for the development of intelligent antioxidant drugs. Using different stimuli-responsive materials, researchers have developed various smart biomimetic GPx with different responsive catalytic activity, such as temperature responsive, [27][28][29][30][31][32][38][39][40] pH responsive, 41 photo responsive, [41][42][43] ATP responsive 19 and so on. In particular, the construction of temperature responsive biomimetic GPx and the exploration of temperature responsive catalytic mechanisms have been systematically studied.…”