Construction of a Carcinoembryonic Antigen Surface-Enhanced Raman Spectroscopy (SERS) Aptamer Sensor Based on the Silver Nanorod Array Chip

Li, Rui; Li, Lijun; Zhang, Yan; Lin, Xin; Guo, Heyuanxi; Lin, Chubing; Feng, Jun

doi:10.1177/00037028221131577

Cited by 6 publications

(2 citation statements)

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“…When combined with the specificity and versatility (here, Raman-active labeling) of nucleic acid aptamers, the technique was able to reach low limits of detection (that could challenge the limits of PCR techniques) as shown for SARS-CoV-2 [163,164] as well as the influenza A virus [165,166]. SERS was also applied recently for the sensitive detection of cancer biomarkers, such as carcinoembryonic antigen (CEA) [167]. In particular, Zhang et al have shown valuable improvement in the utilization of SERS through the detection of cancer cell-derived exosomes in clinical serum samples [168].…”

Section: Optical Aptasensorsmentioning

confidence: 99%

Aptamers Targeting Membrane Proteins for Sensor and Diagnostic Applications

et al. 2023

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Many biological processes (physiological or pathological) are relevant to membrane proteins (MPs), which account for almost 30% of the total of human proteins. As such, MPs can serve as predictive molecular biomarkers for disease diagnosis and prognosis. Indeed, cell surface MPs are an important class of attractive targets of the currently prescribed therapeutic drugs and diagnostic molecules used in disease detection. The oligonucleotides known as aptamers can be selected against a particular target with high affinity and selectivity by iterative rounds of in vitro library evolution, known as Systematic Evolution of Ligands by EXponential Enrichment (SELEX). As an alternative to antibodies, aptamers offer unique features like thermal stability, low-cost, reuse, ease of chemical modification, and compatibility with various detection techniques. Particularly, immobilized-aptamer sensing platforms have been under investigation for diagnostics and have demonstrated significant value compared to other analytical techniques. These “aptasensors” can be classified into several types based on their working principle, which are commonly electrochemical, optical, or mass-sensitive. In this review, we review the studies on aptamer-based MP-sensing technologies for diagnostic applications and have included new methodological variations undertaken in recent years.

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Section: Optical Aptasensorsmentioning

confidence: 99%

Aptamers Targeting Membrane Proteins for Sensor and Diagnostic Applications

et al. 2023

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“…As such, identifying the CEA levels holds significant clinical value in tumor diagnosis. Various sensing approaches have been suggested for CEA detection and monitoring, including fluoroimmunoassay, enzyme-linked immunosorbent assay (ELISA), Raman spectroscopy, radioimmunoassay, and surface plasmon resonance . However, target detection following such sensing approaches commonly requires long processing times, expensive instruments, and labeled markers .…”

Section: Introductionmentioning

confidence: 99%

DNA-Coupled AuNPs@CuMOF for Sensitive Electrochemical Detection of Carcinoembryonic Antigen

Bahri,

Fredj,

Qin

et al. 2024

ACS Appl. Nano Mater.

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Detecting preinvasive tumors before the onset of advanced clinical symptoms represents a critical advancement in improving the efficacy of early medical interventions and reducing cancer-related mortality rates. Immunosensors have emerged as the preferred choice for analyzing oncomarkers and meeting the everincreasing demands of medical diagnostics. Therefore, the quest for the development of highly specific and sensitive immunosensors remains most sought-after to open new avenues for personalized healthcare and medical monitoring. Here, we report amplification-free and nonlabeled DNA framework-coupled AuNPs@CuMOF for ultrasensitive and highly specific electrochemical detection of carcinoembryonic antigen (CEA). Upon the CEA concentration variation from 0.0001 to 200 ng mL −1 , the proposed biosensor led to a limit of detection (LOD) of 0.25 pg mL −1 , surpassing most previously published CEA biosensors that rely on amplification-free methods and aligns with those based on amplification techniques. Furthermore, the suggested CEA immunosensor demonstrated auspiciously high specificity and sensitivity in detecting CEA in human serum comparable to the commercial ELISA within a relative error falling between 6.25% and 2.26%. The proposed analytical approach, hinging on the synergy effect of the CuMOF's large surface area and the mechanical rigidity of the three-dimensional tetrahedron DNA structured probes (3D TDNA), obviously broadens the potential of immunosensors, offering higher sensitivity and lower LOD. Therefore, this work is not only an expansion of CuMOF exploitation in electrochemical biosensing but also a major milestone in detecting diverse biomarkers in clinical settings for tumor diagnosis.

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