Construction of a DNA vaccine and its protective effect on largemouth bass (Micropterus salmoides) challenged with largemouth bass virus (LMBV)

Yi, Wanying; Zhang, Xin; Zeng, Ke; Xie, Daofa; Chen, Song; Tam, Kachon; Liu, Zijing; Zhou, Tianhong; Li, Wei

doi:10.1016/j.fsi.2020.06.062

Cited by 50 publications

(22 citation statements)

References 34 publications

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“…Results from the present study showed that the antibody titers reached up to 64 at 28 dpi, and the RPS of fish orally immunized with GS115-pW317-MCPD was 41.6%. However, Yi et al (21) reported that SN antibody titers could reach a peak with the value of 1:375 ± 40 at 14 days after immunization via injection, which was almost 6-fold higher than our results. This may be because the immunological effect of injection was better than oral administration.…”

Section: Discussioncontrasting

confidence: 95%

Oral Vaccination With Recombinant Pichia pastoris Expressing Iridovirus Major Capsid Protein Elicits Protective Immunity in Largemouth Bass (Micropterus salmoides)

et al. 2022

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Largemouth bass iridovirus (LMBV) can cause high mortality and lead to heavy economic loss in the cultivation of largemouth bass, but there was no effective treatment. Here, the present study constructed a recombinant Pichia pastoris expressing LMBV major capsid protein (MCPD). The recombinant GS115-pW317-MCPD was then used to immunize largemouth bass via oral administration, and mucosal immune response mediated by immunoglobulins (Igs) was measured after oral immunization. Serum antibody levels were measured by ELISA, neutralizing antibody titers were determined by serum neutralization test (SNT), antigen presentation-related gene expressions were detected by RT-PCR, and the histopathological characteristics of immunized fish were assessed after challenging with 0.1 ml 107.19 TCID50/ml LMBV. The relative percentage survival (RPS) was also determined. Our results showed that the serum antibody titers of immunized fish were significantly higher than that of control groups (P < 0.05). IgT and IgM expressions in gut were increased significantly after vaccination with GS115-pW317-MCPD; however, much stronger response in gut was observed as compared with gill. The expression levels of major histocompatibility complex (MHC) II, CD8, and T-cell receptor (TCR) were significantly elevated in GS115-pW317-MCPD group (P < 0.05), while CD4 and MHC I transcription levels remained unchanged after oral immunization (P > 0.05). The RPS of fish orally immunized with 1.0 × 108 CFU/g GS115-pW317-MCPD was reached up to 41.6% after challenge with 0.1 ml 109.46 TCID50/ml LMBV. Moreover, orally immunizing with GS115-pW317-MCPD can relieve the pathological damage caused by LMBV. Therefore, GS115-pW317-MCPD showed a promising potential against LMBV.

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Section: Discussioncontrasting

confidence: 95%

Oral Vaccination With Recombinant Pichia pastoris Expressing Iridovirus Major Capsid Protein Elicits Protective Immunity in Largemouth Bass (Micropterus salmoides)

et al. 2022

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“…Largemouth bass ( Micropterus salmoides ) is a fast-growing aquaculture species in China, with production exceeding 0.61 million tons in 2020 [ 21 ]. However, disease is currently a major factor restricting the development of largemouth bass culture [ 22 , 23 ]. Functional additives are an effective way to promote growth and increase antioxidant defense in largemouth bass.…”

Section: Introductionmentioning

confidence: 99%

Tolerance Assessment of Atractylodes macrocephala Polysaccharide in the Diet of Largemouth Bass (Micropterus salmoides)

Dong

Chen

et al. 2022

Antioxidants

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Atractylodes macrocephala polysaccharide (AMP) can enhance antioxidant defense and anti-inflammation, as the tolerance levels of AMP in aquaculture is important for additive utilization. However, the tolerance dose of AMP is unknown. We assess the tolerance levels of AMP in juvenile largemouth bass (3.38 ± 0.11 g) by feeding them a 0, 400, 4000, or 8000 mg/kg AMP supplemented diet for 10 weeks. The 400 mg/kg AMP dose increased growth performance. The Nrf2/Keap1 signaling pathway was activated, as indicated by Keap1 and Nrf2 protein levels in the liver. Enhanced activity of antioxidant enzymes (SOD, GPx), together with increased mRNA levels of antioxidant genes (sod, gpx) and decreased accumulation of reactive oxygen species (ROS) and MDA, was found in the liver, implying the antioxidant effect of AMP. Nutrient absorption was enhanced by AMP, as reflected by the increased length of intestinal villi and microvilli. However, 4000 and 8000 mg/kg AMP induced oxidant stress, as indicated by increased plasma ALT and AST content and decreased mRNA levels of antioxidant genes (sod, gpx) in the liver and intestinal tissues. Inflammatory reactions were also induced by high doses of AMP, as reflected by enhanced levels of pro-inflammatory cytokines (tnfα, nfκb) in the liver, intestinal, and kidney tissues and inhibited levels of anti-inflammatory cytokines (tgfβ, iκb). Histological analysis reveals inflammatory cell infiltration and tissue damage. Thus, the safe tolerance margin of AMP supplement for largemouth bass was 400–4000 mg/kg.

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“…Largemouth bass (Micropterus salmoides) is an economically important freshwater fish species, and it has been widely cultured globally (Yi et al, 2020). With the culture scale increasing, the diseases caused by pathogens also increased which cause great economic losses and do great harm to the culture industry of largemouth bass (Jia et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization and expression analysis of three interleukins (IL‐1β, IL‐15 and IL‐16) in largemouth bass (Micropterus salmoides)

Zhang

et al. 2022

J Appl Ichthyol

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Interleukins (ILs) are a subgroup of cytokines involved in the intercellular regulation of the immune system, which play important roles in host immune defense against pathogens. In the present study, three ILs (IL-1β, IL-15 and IL-16) were identified from largemouth bass (Micropterus salmoides), an economically important freshwater fish species. The three ILs, which shared characteristic features with their counterparts in other fish species, were ubiquitously expressed in all the examined tissues, but with different expression patterns. Following lipopolysaccharide (LPS) and polyinosinicpolycytidylic acid (polyI:C) stimulation, all the three genes were up-regulated to different degrees in spleen of largemouth bass. The results indicated that these ILs involved in the immune response of largemouth bass during bacterial and virus infection. To the best of our knowledge, this is the first characterization of these three genes in largemouth bass. Our results will not only enrich the understanding of interleukins in largemouth bass, but also provide a valuable framework for further studies of the immune function of these interleukins during pathogen invasion.

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Construction of a DNA vaccine and its protective effect on largemouth bass (Micropterus salmoides) challenged with largemouth bass virus (LMBV)

Cited by 50 publications

References 34 publications

Oral Vaccination With Recombinant Pichia pastoris Expressing Iridovirus Major Capsid Protein Elicits Protective Immunity in Largemouth Bass (Micropterus salmoides)

Oral Vaccination With Recombinant Pichia pastoris Expressing Iridovirus Major Capsid Protein Elicits Protective Immunity in Largemouth Bass (Micropterus salmoides)

Tolerance Assessment of Atractylodes macrocephala Polysaccharide in the Diet of Largemouth Bass (Micropterus salmoides)

Molecular characterization and expression analysis of three interleukins (IL‐1β, IL‐15 and IL‐16) in largemouth bass (Micropterus salmoides)

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