Construction of a fluorescence sensing platform based on RISC and CRISPR/Cas12a for the assay of Ago2 enzyme activity

Wen, Xiaoqing; Zhuo, Chenyi; Wei, Jihua; Gong, Yuanxun; Tang, Qianli; Liao, Xianjiu; Zhang, Kai

doi:10.1016/j.microc.2023.109284

Cited by 3 publications

(2 citation statements)

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“…The CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats)-Cas (CRISPR-associated) system originated from the adaptive immune system of prokaryotes, providing defense against invading nucleic acids. [13][14][15] In recent years, the CRISPR-Cas system has garnered signi cant attention from biological researchers as a highly effective gene editing tool. [13,[15][16][17][18] Broadly, CRISPR/Cas systems can be categorized into Class I and Class II, based on whether they use single or multiple effectors.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemiluminescence Biosensor for MMP-2 Analysis Using CRISPR/Cas13a and EXPAR Amplification: A Novel Approach for Anti-Aging Research

Tang,

Wang,

Zhang

et al. 2024

Preprint

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Matrix metalloproteinase-2 (MMP-2) plays a pivotal role in anti-aging research. Developing advanced detection platforms for MMP-2 with high specificity, sensitivity, and accessibility is crucial. This study introduces a novel electrochemiluminescence (ECL) biosensor for MMP-2 analysis, leveraging the CRISPR/Cas13a system and Exponential Amplification Reaction (EXPAR). The biosensor operates by utilizing the T7 RNA polymerase to transcribe RNA from a DNA template upon MMP-2 interaction. This RNA activates Cas13a, leading to signal amplification and ECL detection. The incorporation of the "photoswitch" molecule [Ru(phen)2dppz]2+ streamlines the process by eliminating the need for extensive electrode modification and cleaning. Under optimized conditions, the biosensor achieved an impressive detection limit of 12.8 aM for MMP-2. The platform demonstrated excellent selectivity, reproducibility, and stability, making it highly suitable for detecting MMP-2 in complex biological samples. This innovative approach shows great potential for applications in molecular diagnostics and anti-aging research.

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Section: Introductionmentioning

confidence: 99%

“…[13][14][15] In recent years, the CRISPR-Cas system has garnered signi cant attention from biological researchers as a highly effective gene editing tool. [13,[15][16][17][18] Broadly, CRISPR/Cas systems can be categorized into Class I and Class II, based on whether they use single or multiple effectors.…”

Section: Introductionmentioning

confidence: 99%