Construction of a Full-Length cDNA Library of Gossypium hirsutum L. and Identification of Two MADS-Box Genes

Wang, Lina; Wang, Dong; Yu, Shuang; Fan, Shiliang; Song, Meizhen; Pang, Chaoyou; Liu, Junjie

doi:10.1016/s1671-2927(11)60304-0

Cited by 1 publication

(1 citation statement)

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“…Some reports have proven that projects based on ESTs are powerful tools for both the analysis of gene expression patterns in a given tissue or at specific developmental stages (Chen et al, 2012;Tran et al, 2011;Wang et al, 2011b;Yang et al, 2009b;Zhang et al, 2011a). Moreover, ESTs from complementary DNA (cDNA) clones are inexpensive and efficient gene discovery tools (Wang et al, 2011a;Xiao et al, 2011;Yamagishi et al, 2011). As a molecular basis of information on whole genomes, the accumulation of ESTs is a promising strategy for studies in plant molecular biology (Rudd, 2003).…”

Section: Introductionmentioning

confidence: 99%

Construction of a full-length cDNA library and analysis of expressed sequence tags in white jute (Corchorus capsularis L.)

Tao¹,

Li²,

Qi³

et al. 2015

Afr. J. Biotechnol.

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White jute (Corchorus capsularis L.) is recognized as an important industrial raw material fibre crop owing to its elite characters. However, little information is known about its molecular basis and genomics. In this study, a complementary DNA library of white jute was constructed and expressed sequence tags (ESTs) were characterized. The titers of original and amplified libraries were 2.32 × 10 7 and 1.07 × 10 9 pfu/mL, respectively. The recombinant frequency was 98.3% in the library. Most of the sequences ranged from 500 to 1500 bp with an average length of 750 bp. Results show 203 (73%) ESTs exhibited significant similarity with known or putative functional nucleotide sequences in the GenBank databases. Cluster analysis allowed the identification of 61 unique sequences. These genes were classified into six types by Gene Ontology (GO) annotation. The results also indicated that unigenes of C. capsularis have higher homology to Populus trichocarpa, Ricinus communis and Corchorus olitorius. This report will provide a valuable resource for the further investigations in the gene cloning, transcription or expression for white jute.

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