Construction of A High‐Flux Protein Transport Channel Inspired by the Nuclear Pore Complex

Abstract

Inspired by the nuclear pore complex (NPC), herein we have established a biomimetic high-flux protein delivery system via the ingenious introduction of pillar [5]arene-based host-guest system into one side of artificial hour-glass shaped nanochannel. With a transport flux of 660 lysozymes per minute, the system provides efficient high-flux protein transport at a rate which is significantly higher than that of an unmodified nanochannel and conventional bilateral symmetrical modified nanochannels. In view of these promising results, the use of artificial nanochannel to improve protein transport not only presents a new potential chemical model for biological research and better understanding of protein transport behavior in the living systems, but also provides a highflux protein transporter device, which may have applications in the design of protein drug release systems, protein separation systems and microfluidics in the near future.

Orientational Pathways during Protein Translocation through Polymer-Modified Nanopores

“…Protein transport through nanopores and nanochannels is also highly relevant in other applications beyond sensing. Micrometer-long nanochannels were chemically modified to prepare high-flux protein transporters with potential applications in biomolecular delivery. , Enzyme confinement in silica nanopores resulted in biocatalytic materials. − Polymer nanopores have been used to prepare membranes for the separation of protein mixtures. , …”