Construction of a molecular genetic map for hawthorn based on SRAP markers

Wang, Gang; Guo, Yinshan; Zhao, Yuhui; Zhang, Jijun

doi:10.1080/13102818.2015.1018322

Cited by 4 publications

(4 citation statements)

References 20 publications

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“…SRAPs are dominant markers in nature. Generation of SRAP data and its analysis reveal valuable information for assessing genetic diversity of large germplasm collections and identification of quantitative trait loci in different taxa ( Wanga et al., 2015 ). As an ORF-based marker that targets functional genes, SRAP is very valuable in marker assisted crop breeding and improvement ( Bhatt et al., 2017 ; Ge and Daizhen 2015 ).…”

Section: The Concept Of Molecular or Dna Markersmentioning

confidence: 99%

Basic concepts and methodologies of DNA marker systems in plant molecular breeding

Amiteye

2021

Heliyon

131

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The concepts, methodologies and applications of some of the major molecular or DNA markers commonly used in plant science have been presented. The general principles of molecular marker techniques have been elucidated with detailed explanation of some notable basic concepts associated with marker applications: marker polymorphism, dominant or co-dominant mode of inheritance, agronomic trait-marker linkage, genetic mutations and variation. The molecular marker methods that have been extensively reviewed are RFLP, RAPD, SCAR, AFLP, SSR, CpSSR, ISSR, RAMP, SAMPL, SRAP, SSCP, CAPS, SNP, DArT, EST, and STS. In addition, the practicality of the retrotransposon-based marker methods, IRAP, REMAP, RBIP, and IPBS, have been discussed. Moreover, some salient characteristics of DNA markers have been compared and the various marker systems classified as PCR-or non-PCR-based, dominantly or co-dominantly inherited, locus specific or non-specific as well as at the levels of marker polymorphism and efficiency of marker reproducibility. Furthermore, the principles and methods of the following DNA markers have been highlighted: Penta-primer amplification refractory mutation system (PARMS), Conserved DNA-Derived Polymorphism (CDDP), P450-based analogue (PBA) markers, Tubulin-Based Polymorphism (TBP), Inter-SINE amplified polymorphism (ISAP), Sequence specific amplified polymorphism (S-SAP), Intron length polymorphisms (ILPs), Inter small RNA polymorphism (iSNAP), Direct amplification of length polymorphisms (DALP), Promoter anchored amplified polymorphism (PAAP), Target region amplification polymorphism (TRAP), Conserved region amplification polymorphism (CoRAP), Start Codon Targeted (SCoT) Polymorphism, and Directed Amplification of Minisatellite DNA (DAMD). Some molecular marker applications that have been recently employed to achieve various objectives in plant research have also been outlined. This review will serve as a useful reference resource for plant breeders and other scientists, as well as technicians and students who require basic know-how in the use of molecular or DNA marker technologies.

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Section: The Concept Of Molecular or Dna Markersmentioning

confidence: 99%

Basic concepts and methodologies of DNA marker systems in plant molecular breeding

Amiteye

2021

Heliyon

131

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“…To date, there are only two reports on genetic map construction in hawthorn. Wang et al [23] first constructed the genetic maps for hawthorn cultivars 'Damianqiu' and 'Qiujinxing' by using SRAP markers and 92 hybrid offspring. However, the number of markers in these genetic maps was very low, and the average genetic distance between adjacent markers was very large, which was not suitable for fine QTL mapping.…”

Section: The Advantage Of Snp Marker and High Density Linkage Map Conmentioning

confidence: 99%

“…However, the number of markers in these genetic maps was very low, and the average genetic distance between adjacent markers was very large, which was not suitable for fine QTL mapping. Furthermore, SRAP markers also showed poor repeatability and stability in linkage map construction [23] and not considerable for constructing genetic maps of other hawthorn cultivars. Zhao et al constructed other genetic maps by using 107 hybrid offspring of the same population and 2b-RAD technology and a total of 3894 SNP markers were achieved in the integrated map [24].…”

Section: The Advantage Of Snp Marker and High Density Linkage Map Conmentioning

confidence: 99%

High-density genetic linkage-map construction of hawthorn and QTL mapping for important fruit traits

Zhao

Guo

et al. 2020

PLoS ONE

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Few reports exist on QTL mapping of the important economic traits of hawthorn. We hybridized the cultivars 'Shandongdamianqiu' (female parent) and 'Xinbinruanzi' (male parent), and 130 F 1 individuals and the two parents were used for RAD-seq, SNP development, and high-density linkage map construction. Three genetic maps were obtained, one for each of the parents and an integrated one. In these three maps, 17 linkage groups were constructed. The female and male parent maps contained 2657 and 4088 SNP markers, respectively, and had genetic distances of 2689.65 and 2558.41 cM, respectively, whereas the integrated map was 2470.02 cM, and contained 6,384 SNP markers. QTL mapping based on six agronomic traits, namely fruit transverse diameter, vertical diameter, single fruit weight, pericarp brittleness, pericarp puncture hardness, and average sarcocarp firmness were conducted, and 25 QTLs were detected in seven linkage groups. Explained phenotypic variation rate ranged from 17.7% to 35%. This genetic map contains the largest number of molecular markers ever obtained from hawthorn and will provide an important future reference for fine QTL mapping of economic traits and molecular assisted selection of hawthorn. et al. (2020) High-density genetic linkage-map construction of hawthorn and QTL mapping for important fruit traits. PLoS ONE 15(2): e0229020.

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“…However, the relatively insufficient genetic mapping limits the molecular-assisted breeding of hawthorn to some extent. Wang G. et al (2015) constructed a rough genetic map of hawthorn using SRAP markers, with fewer than 100 markers and an average distance of more than 5 cM. Wang (2016) constructed a genetic map of hawthorn plants by using both SRAP and SSR markers, and the marker distance of male and female parents were 7.05 cM and 6.45 cM, respectively.…”

Section: Introductionmentioning

confidence: 99%

High-density genetic map and quantitative trait loci map of skin color in hawthorn (Crataegus pinnatifida bge. Var. major N.E.Br.)

Wang,

Cheng,

Zhang

2024

Front. Genet.

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Fruit skin color is an important trait of the hawthorn tree, which has an important influence on fruit quality. Crataegus pinnatifida Bge. var. Major N.E.Br. Is one of the most widely cultivated varieties in China and has a long history of medicinal use. In recent years, it has attracted the attention of the world due to its nutritional and medicinal values. Skin color is the focus of breeders and food processors. At present, skin color-related genes have still not been mapped. In this study, “Shandong Da Mianqiu” (♀, red skin color), “Da Huang Mianzha” (♂, yellow skin color) and 131 F1 hybrids were used to construct genetic map of hawthorn by RAD-seq, and QTL mapping was performed by combining these features with the hue angle and the observed color. In this study, 13,260 SNP was assigned to 17 linkage groups, with an integrated map covering 2,297.75 cM was constructed. A total of 5 QTLs related to hawthorn skin color were detected on LG1, LG3 and LG15. Whether hue angle or pericarp color acts as phenotype for QTL mapping, the candidate genes include bHLH086, WD repeat regions and Myb-like. bHLH, WD and Myb play an important role in the color regulation of Hawthorn skin color. These results lay a solid foundation for QTL mapping and molecular marker-assisted breeding of hawthorn.

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Construction of a molecular genetic map for hawthorn based on SRAP markers

Cited by 4 publications

References 20 publications

Basic concepts and methodologies of DNA marker systems in plant molecular breeding

Basic concepts and methodologies of DNA marker systems in plant molecular breeding

High-density genetic linkage-map construction of hawthorn and QTL mapping for important fruit traits

High-density genetic map and quantitative trait loci map of skin color in hawthorn (Crataegus pinnatifida bge. Var. major N.E.Br.)

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