Construction of a PANoptosis-Related LncRNA Prognostic Model and Study on the Function of Linc01135 for Gastric Cancer

Abstract

Objective Our study aimed to construct a predictive model for gastric cancer using PANoptosis-related LncRNAs. Additionally, the function of the model crucial molecule Linc01135 in gastric cancer cells will be explored. Materials and Methods We performed multiple Bioinformatics methods from the Cancer Genome Atlas (TCGA) database to develop a prediction signature. Fluorescence in situ hybridization (FISH) technology was utilized to analyze the localization of Linc01135. In addition, the function of Linc01135 on gastric cancer was assessed through various assays including CTG, colony formation, and scratch experiments. Finally, to identify specific proteins that bind with Linc01135, RNA Pulldown and Ribosome Immunoprecipitation (RIP) experiments were conducted. Result We developed a prognostic model for gastric cancer comprising of 7 lncRNAs. This model effectively distinguishes between high-risk and low-risk populations, as well as accurately predicts survival status. The subcellular localization of Linc01135 was found located in nucleus of HGC-27 cells. In terms of functionality, the knockdown of Linc01135 resulted in a significant reduction in the proliferation and migration abilities of gastric cancer cells. Mechanistically, Linc01135 exhibited specific binding affinity towards CDC45. Ultimately, rescue experiments demonstrated that overexpression of CDC45 partially counteracted the effects caused by knockdown of linc01135 on the proliferation and migration abilities of gastric cancer cells. Conclusion A predictive model based on PANoptosis-Related LncRNA can evaluate prognosis