Construction of a peptide with an electroactive daunomycin like a pendant arm to detect ovalbumin

Monitoring of the interaction between U937 cells and electroactive daunomycin with an arginine-rich peptide

“…The ratio of 2150.78 to 1769.46 became greater, and each peak intensity was decreased by a reduction in the output of the laser light. The phenomenon was observed in the mass spectra of a peptide probe for the detection of ovalbumin [29]. The signal of 1769.46 was due to a fragment of the peptide probes with a hydroxyl group at the 1-position in a carbohydrate moiety of daunomycin.…”

“…Suppression of the by-product was attempted using a reaction process. As a control, daunomycin was bound to peptide-2 (RNRCKGTDVQAW), which is known to recognize ovalbumin [29]. The daunomycin/peptide-2 probe scarcely penetrated the U937 cell membrane.…”

The effect of porosity and stiffness of glutaraldehyde cross-linked egg white scaffold simulating aged extracellular matrix on distribution and aggregation of ovarian cancer cells

“…EW is a kind of medium containing water, with protein as colloidal substance of its dispersed phase, which accounts for about 60% of the total weight of eggs; 85% to 89% is water, and, of the rest of the solids, about 80% is protein [20]. As the cell culture medium, EW contains the complete nutritional and signal components of development that are needed, its mechanical properties and morphology can be widely modified [21,22]. EW especially easily could be obtained economically.…”

Electrochemical Affinity Biosensors in Food Safety

“…Worth to mention also the increasing tendency to fabricate these electrochemical biosensors onto screen-printed electrodes (SPEs) [85,[95][96][97][98][99][100][101][102][103][104]107,109,112,113] since they are suitable to be fabricated with different materials, to be drawn in diverse geometries and multiplexed formats and Recently, Sugawara et al [116] developed an electrochemical biosensor to detect ovalbumin (OVA) by adsorbing a specific peptide probe (peptide-1) conjugated with the electroactive daunomycin on a GCE. In the presence of OVA, the peptide probe was released from the electrode surface and the DPV peak current of the daunomycin moiety decreased.…”

“…Recently, Sugawara et al [116] developed an electrochemical biosensor to detect ovalbumin (OVA) by adsorbing a specific peptide probe (peptide-1) conjugated with the electroactive daunomycin on a GCE. In the presence of OVA, the peptide probe was released from the electrode surface and the DPV peak current of the daunomycin moiety decreased.…”