Construction of a Saccharomyces cerevisiae strain with a high level of RNA

Chuwattanakul, Varesa; Kim, Yeon-Hee; Sugiyama, Minetaka; Nishiuchi, Hiroaki; Miwa, Haruhumi; Kaneko, Yoshinobu; Harashima, Satoshi

doi:10.1016/j.jbiosc.2011.03.011

Cited by 15 publications

(21 citation statements)

References 18 publications

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“…Strain SupD (SH6975; Drrn10 SUPD) was isolated as a suppressor of the growth defect of the Drrn10 disruptant (SH6789) (11). Yeast strains were grown at 30 C in yeast peptone dextrose adenine (YPDA) medium or synthetic complete (SC) medium, as described by Amberg et al (12).…”

Section: Methodsmentioning

confidence: 99%

“…As an internal control, ACT1 mRNA was detected using ACT1-F and ACT1-R (Table 2) as the forward and reverse primers, respectively. The total amount of RNA in the yeast cells was measured using the perchloric acid method according to Chuwattanakul et al (11).…”

Section: Plasmid Constructionmentioning

confidence: 99%

“…Importantly, the introduction of the RRN10 gene into the originally isolated suppressors that carried the Drrn10 disruption led to 1.4-to 2.3-fold higher RNA content than the wild-type strain, demonstrating that our strategy was effective for the development of a strain capable of producing a higher amount of RNA (11).…”

mentioning

confidence: 91%

“…Rrn10p, one of the components of UAF, is essential to promote a high level of transcription of the 35S rRNA gene; therefore, the disruption of the RRN10 gene causes a specific defect in the Pol I-mediated transcription of rDNA, leading to extremely slow growth and a small-colony phenotype (11). To create a strain that shows a higher RNA content for the food industry, suppressors were isolated from the Drrn10 disruptant and genetically categorized into seven groups (SupA to SupG) (11).…”

mentioning

confidence: 99%

“…To create a strain that shows a higher RNA content for the food industry, suppressors were isolated from the Drrn10 disruptant and genetically categorized into seven groups (SupA to SupG) (11). Our previous study revealed that all of the suppressors contained dominant and multiple mutations (hereafter, those multiple mutations in the SupA to SupG strains are collectively designated SUPA to SUPG), each of which were required to suppress the growth defect caused by the disruption of the RRN10 gene and conferred the phenotype of a higher RNA content than the Drrn10 disruptant strain (11).…”

mentioning

confidence: 99%

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Increased transcription of NOP15, involved in ribosome biogenesis in Saccharomyces cerevisiae, enhances the production yield of RNA as a source of nucleotide seasoning

Chuwattanakul

Sugiyama

Khatun

et al. 2012

Journal of Bioscience and Bioengineering

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Section: Methodsmentioning

confidence: 99%

Section: Plasmid Constructionmentioning

confidence: 99%

mentioning

confidence: 91%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Increased transcription of NOP15, involved in ribosome biogenesis in Saccharomyces cerevisiae, enhances the production yield of RNA as a source of nucleotide seasoning

Chuwattanakul

Sugiyama

Khatun

et al. 2012

Journal of Bioscience and Bioengineering

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Improving ribonucleic acid production in Saccharomyces pastorianus via in silico genome‐scale metabolic network model

Chen

Wang

et al. 2023

Biotechnology Journal

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Ribonucleic acid (RNA) and its degradation products are important biomolecules widely used in the food and pharmaceutical industries for their flavoring and nutritional functions. In this study, we used a genome‐scale metabolic network model (GSMM) to explore genetic targets for nucleic acid synthesis in a Saccharomyces pastorianus strain (G03). Yeast 8.5.0 was used as the base model, which accurately predicted G03's growth. Using OptForce, we found that overexpression of ARO8 and ATP1 among six different strategies increased the RNA content of G03 by 58.0% and 74.8%, respectively. We also identified new metabolic targets for improved RNA production using a modified GSMM called TissueModel, constructed using the GIMME transcriptome constraint tool to remove low‐expressed reactions in the model. After running OptKnock, the RNA content of G03‐△BNA1 and G03‐△PMA1 increased by 44.6% and 39.8%, respectively, compared to G03. We suggest that ATP1, ARO8, BNA1, and PMA1 regulate cell fitness, which affects RNA content. This study is the first to identify strategies for RNA overproduction using GSMM and to report that regulation of ATP1, ARO8, BNA1, and PMA1 can increase RNA content in S. pastorianus. These findings also provide valuable knowledge on model reconstruction for S. pastorianus.

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Increase in rRNA content in a Saccharomyces cerevisiae suppressor strain from rrn10 disruptant by rDNA cluster duplication

Khatun

Sasano

Sugiyama

et al. 2013

Appl Microbiol Biotechnol

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Construction of a Saccharomyces cerevisiae strain with a high level of RNA

Cited by 15 publications

References 18 publications

Increased transcription of NOP15, involved in ribosome biogenesis in Saccharomyces cerevisiae, enhances the production yield of RNA as a source of nucleotide seasoning

Increased transcription of NOP15, involved in ribosome biogenesis in Saccharomyces cerevisiae, enhances the production yield of RNA as a source of nucleotide seasoning

Improving ribonucleic acid production in Saccharomyces pastorianus via in silico genome‐scale metabolic network model

Increase in rRNA content in a Saccharomyces cerevisiae suppressor strain from rrn10 disruptant by rDNA cluster duplication

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