Construction of a tissue-specific transcription factor-tethered extracellular matrix protein via coiled-coil helix formation

Siew, SokeLee; Kaneko, Mami; Mie, Masayasu; Kobatake, Eiry

doi:10.1039/c5tb01579k

Cited by 9 publications

(7 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, we speculate that EuNAC10 in the same subgroup is a drought and high salt responsive gene, which regulates the survival of E. ulmoides under adverse growth conditions. In addition, transcription factors usually play a key role in controlling the expression of tissue-specific genes (Choi et al, 2006;Lim et al, 2012;Siew et al, 2016). This study provides useful clues for understanding gene function concerning specific processes.…”

Section: Discussionmentioning

confidence: 95%

See 1 more Smart Citation

The NAC transcription factor family in Eucommia ulmoides: Genome-wide identification, characterization, and network analysis in relation to the rubber biosynthetic genes

Zhang

Ren

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

The NAC transcription factor family is a large plant gene family, participating in plant growth and development, secondary metabolite synthesis, biotic and abiotic stresses responses, and hormone signaling. Eucommia ulmoides is a widely planted economic tree species in China that can produce trans-polyisoprene: Eucommia rubber (Eu-rubber). However, genome-wide identification of the NAC gene family has not been reported in E. ulmoides. In this study, 71 NAC proteins were identified based on genomic database of E. ulmoides. Phylogenetic analysis showed that the EuNAC proteins were distributed in 17 subgroups based on homology with NAC proteins in Arabidopsis, including the E. ulmoides-specific subgroup Eu_NAC. Gene structure analysis suggested that the number of exons varied from 1 to 7, and multitudinous EuNAC genes contained two or three exons. Chromosomal location analysis revealed that the EuNAC genes were unevenly distributed on 16 chromosomes. Three pairs of genes of tandem duplicates genes and 12 segmental duplications were detected, which indicated that segmental duplications may provide the primary driving force of expansion of EuNAC. Prediction of cis-regulatory elements indicated that the EuNAC genes were involved in development, light response, stress response and hormone response. For the gene expression analysis, the expression levels of EuNAC genes in various tissues were quite different. To explore the effect of EuNAC genes on Eu-rubber biosynthesis, a co-expression regulatory network between Eu-rubber biosynthesis genes and EuNAC genes was constructed, which indicated that six EuNAC genes may play an important role in the regulation of Eu-rubber biosynthesis. In addition, this six EuNAC genes expression profiles in E. ulmoides different tissues were consistent with the trend in Eu-rubber content. Quantitative real-time PCR analysis showed that EuNAC genes were responsive to different hormone treatment. These results will provide a useful reference for further studies addressing the functional characteristics of the NAC genes and its potential role in Eu-rubber biosynthesis.

show abstract

Section: Discussionmentioning

confidence: 95%

“…In addition, transcription factors usually play a key role in controlling the expression of tissue-specific genes ( Choi et al., 2006 ; Lim et al., 2012 ; Siew et al., 2016 ). This study provides useful clues for understanding gene function concerning specific processes.…”

Section: Discussionmentioning

confidence: 99%

The NAC transcription factor family in Eucommia ulmoides: Genome-wide identification, characterization, and network analysis in relation to the rubber biosynthetic genes

Zhang

Ren

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…Materials for tissue engineering and regenerative medicine mimic the extracellular matrix (ECM) in order to promote cell adhesion and proliferation within three‐dimensional networks. [ 106,212–222 ] Self‐assembled peptides for tissue engineering applications must exhibit emergent viscoelastic properties that enable the formation of hydrogel networks that physically support cells as well as biochemical properties that enable cellular adhesion to the network. For example, self‐assembled β‐sheet peptides have been used as very effective ECM‐mimetic materials.…”

Section: Applicationsmentioning

confidence: 99%

Multivalent display of chemical signals on self‐assembled peptide scaffolds

et al. 2021

View full text Add to dashboard Cite

Self-assembled peptide materials have emerged as promising bioinspired tools for applications that include regenerative medicine, drug delivery, antimicrobial and vaccine development, optics, and catalysis. Peptide self-assembly mediated by noncovalent hydrogen bonding, coulombic, hydrophobic, and aromatic interactions gives rise to a variety of supramolecular structures that reflect on the nature of the constituent peptides. The emergent properties of these supramolecular peptide materials often depend on the multivalent presentation of functional appendages on the self-assembled scaffold. For example, the multivalent display of cell-signaling motifs on self-assembled peptide nanofibrils provides materials that are excellent extracellular matrix mimetics for tissue engineering applications. This review includes a discussion of chemical strategies that address the challenge of appending functional signal motifs in a multivalent display on self-assembled peptide and protein materials. In addition, recent examples of supramolecular peptide materials that rely on the multivalent display of chemical signals for the desired applications are presented. Collectively, this discussion illustrates the potential of self-assembled peptides as sustainable materials to address challenges in contemporary materials science and provides principles for the design of next-generation agents for a variety of applications.

show abstract

“…It has been exploited for various tissue engineering and gene delivery applications, 18 and several proteins including growth factors, transcription factors and antibodies have been grafted onto polymer-based 5 structures thanks to coiled-coil complexes. [19][20][21][22] Our group previously developed two de novo designed peptides, namely the Ecoil and the Kcoil, which design is based on the repetitions of seven distinct amino acids (a heptad). When mixed together, the peptides spontaneously form a coiled-coil heterodimeric complex, which affinity can be modulated by the number of heptads and their sequence, especially the aliphatic residues that comprise the hydrophobic core of the interaction.…”

Section: Introductionmentioning

confidence: 99%

“…The research based on de novo-designed coiled coil forming peptides has led to a robust knowledge of the determinants of their interactions. − The rational design of peptide sequences that feature tunable specificity, stability, and oligomerization state has therefore been dramatically facilitated. It has been exploited for various tissue engineering and gene delivery applications, and several proteins including growth factors, transcription factors, and antibodies have been grafted onto polymer-based structures thanks to coiled coil complexes. − Our group previously developed two de novo-designed peptides, namely, the Ecoil and the Kcoil, in which designs are based on the repetitions of seven distinct amino acids (a heptad). When mixed together, the peptides spontaneously form a coiled coil heterodimeric complex, in which affinity can be modulated by the number of heptads and their sequence, especially the aliphatic residues that comprise the hydrophobic core of the interaction. − The rationale of this research was to investigate the potential of coiled coil interactions for the preparation of novel hybrid systems, that is, hydrogels that can deliver biofunctionalized NPs (e.g., decorated on their surface with proteins or with encapsulated drugs), at a speed that is not only dependent on particle diffusion or gel degradation but that is tuned by specific affinity interactions.…”

Section: Introductionmentioning

confidence: 99%

Coiled Coil Affinity-Based Systems for the Controlled Release of Biofunctionalized Gold Nanoparticles from Alginate Hydrogels

et al. 2019

View full text Add to dashboard Cite

Affinity-based systems represent a promising solution to control the delivery of therapeutics using hydrogels. Here, we report a hybrid system that is based on the peptidic E/K coiled-coil affinity pair to mediate the release of gold nanoparticles from alginate scaffolds. On the one hand, the gold nanoparticles were functionalized with Ecoil-tagged epidermal growth factor (EGF). The bioactivity of the grafted EGF and the bioavailability of the Ecoil moiety were confirmed by EGF receptor phosphorylation assays and by capturing the functionalized nanoparticles on a Kcoilderivatized surface, respectively. On the other hand, alginate chains were modified with azidohomoalanine Kcoil (Aha-Kcoil) by azide-alkyne click chemistry. The hybrid system was formed by dispersing NPs functionalized with Ecoil-tagged EGF in alginate hydrogels containing either 0, 10 or 20% of Kcoil-modified alginate (Alg-Kcoil). With 20% of Alg-Kcoil, the release of Ecoilfunctionalized NPs was reduced by half when compared to the release of NPs without Ecoil, whereas little to no differences were noticed with either 0 or 10% of Alg-Kcoil. Differential dynamic microscopy was used to determine the diffusion coefficient of the NPs, and the results showed a decrease in the diffusion coefficient of Ecoil-functionalized NPs, when compared to bare pegylated NPs. Altogether, our data demonstrated that the E/K coiled-coil system can control the release of NPs in a high Kcoil content alginate gel, opening diverse applications in drug delivery.

show abstract

Construction of a tissue-specific transcription factor-tethered extracellular matrix protein via coiled-coil helix formation

Abstract: Tissue-specific transcription factor Olig2 was tethered to a designed artificial extracellular matrix proteinviacoiled-coil helix formation.

Cited by 9 publications

References 36 publications

The NAC transcription factor family in Eucommia ulmoides: Genome-wide identification, characterization, and network analysis in relation to the rubber biosynthetic genes

The NAC transcription factor family in Eucommia ulmoides: Genome-wide identification, characterization, and network analysis in relation to the rubber biosynthetic genes

Multivalent display of chemical signals on self‐assembled peptide scaffolds

Coiled Coil Affinity-Based Systems for the Controlled Release of Biofunctionalized Gold Nanoparticles from Alginate Hydrogels

Contact Info

Product

Resources

About