Construction of a triple gene-deleted Chinese Pseudorabies virus variant and its efficacy study as a vaccine candidate on suckling piglets

Zhang, Chaolin; Guo, Linghua; Jia, Xiangrui; Wang, Tongyan; Wang, Juan; Sun, Zhe; Wang, Lilin; Li, Xiangdong; Tan, Feifei; Tian, Kegong

doi:10.1016/j.vaccine.2015.03.094

Cited by 70 publications

(40 citation statements)

References 14 publications

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“…A good example is PRV Barth K-61 vaccine with gE/gI/ US9 gene-deletion that renders the strain incapable of anterograde spread [10] . Besides natural deletions of genes by extensively passaging field isolates in cell cultures, attenuation of PRV is also mediated by deletion of single or multiple virulence-determined genes such as TK, gE and gI by using BAC technology [4] . In PRV, ribonucleotide reductase catalyzes the reduction of ribonucleotides into deoxyribonucleotides, the substrates for DNA synthesis [8] .…”

Section: Discussionmentioning

confidence: 99%

“…The construction of TK/gE/gI triple gene-deleted pseudorabies virus (vPRV) has been described previously [4] . This strategy was used to construct RR gene-deleted virus based on the triple gene-deleted virus.…”

Section: Construction Of Gene-deleted Virusmentioning

confidence: 99%

“…This strategy was used to construct RR gene-deleted virus based on the triple gene-deleted virus. Briefly, pBAC-PRV TK [4] .…”

Section: Construction Of Gene-deleted Virusmentioning

confidence: 99%

“…However, with the emergence of PRV variants, this disease is still prevalent in China and poses a huge threat to the swine industry [3] . As for PRV modified live vaccines, several virulencerelated genes such as thymidine kinase (TK), and glycoproteins glycoprotein E (gE) and glycoprotein I (gI) are commonly targeted for deletion to reduce virulence but keep immunogenicity [4] . By using bacterial artificial chromosome (BAC) technology, double-or triple-gene deleted vaccines were constructed and showed to provide useful protection as vaccine candidates [5,6] .…”

Section: Introductionmentioning

confidence: 99%

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RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

Yan¹,

He²,

Zhang³

et al. 2016

Front. Agr. Sci. Eng.

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As virulence-determining genes, RR1 and RR2 encode the small subunit and large subunit of viral ribonucleotide reductase (RR) in pseudorabies virus which have been extensively studied in mice. However, their role in pigs has not been adequately investigated. In this study, we deleted RR1 and RR2 genes based on a TK/gE/gI triple gene-deleted pseudorabies virus and tested its efficacy in pigs as a vaccine candidate. The rescued virus showed similar growth properties and plaque size in vitro as its parent strain. In an animal study, the virus could elicit humoral immune responses shown by generation of gB-specific antibodies and virus neutralizing antibodies. However, vaccination could not provide protection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific syndromes. The deficiency in protection may due to the generation of late and low levels of gB antibodies and virus neutralizing antibodies.

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Section: Discussionmentioning

confidence: 99%

Section: Construction Of Gene-deleted Virusmentioning

confidence: 99%

“…This strategy was used to construct RR gene-deleted virus based on the triple gene-deleted virus. Briefly, pBAC-PRV TK [4] .…”

Section: Construction Of Gene-deleted Virusmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

Yan¹,

He²,

Zhang³

et al. 2016

Front. Agr. Sci. Eng.

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“…It has been reported that the absence of glycoprotein E (gE), glycoprotein I (gI) and thymidine kinase (TK) attenuates PRV (Mettenleiter, 2003;Zhang et al, 2015). We first designed and constructed three gE-specific CRISPR/Cas9 constructs.…”

Section: Effective Sgrna Screeningmentioning

confidence: 99%

Live attenuated pseudorabies virus developed using the CRISPR/Cas9 system

et al. 2016

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Currently, pseudorabies virus (PRV) variant strains are outbreaking in China; these variants belong to genotype II PRV. The traditional Bartha-K61 vaccine has failed to provide complete protection against the emergent variant strains. Therefore, rapid attenuation of current epidemic strains is needed for effective PRV control. In this study, we report a rapid method for editing the PRV genome using the CRISPR-Cas9 system. We developed a triple gE/gI/TK gene-inactivated HeN1 PRV strain, because mice were more susceptible to PRV infection, we then evaluated the attenuation of PRV in the mice and demonstrated that modified PRV was fully attenuated. Furthermore, the attenuated strain also induced immune protection in response to a parental PRV challenge. Overall, we showed that PRVs can be rapidly attenuated using CRISPR-Cas9 technology, which will be critical for PRV control, especially when new variant PRV strains emerge.

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Carbon‐Based Nanomaterials for Antiviral Applications

Serrano‐Aroca,

Takayama,

Mishra

et al. 2024

Adv Funct Materials

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In the antimicrobial resistance era, carbon‐based nanomaterials (CBNs) such as fullerenes, carbon dots, graphene, and their derivatives are promising therapeutic tools in combating viral diseases. This review shows that these materials have broad‐spectrum antiviral activity against 33 viruses belonging to different Baltimore groups. CBNs also exhibit antimicrobial activity against bacteria and fungi and possess a low risk of selecting for resistance, since their primary mode of antimicrobial action involves physically damaging the microbes. CBNs also offer additional promising properties, including enhanced antiviral effectiveness under diverse types of irradiation and facilitating antiviral immune responses. Their potential as antiviral agents is still in its infancy and future research should focus on their toxicity, antiviral mechanisms, pharmacokinetics, and bioavailability. They are also potential antiviral materials for preventing the transmission of viral diseases for use in face masks, shields, hospital and airport surfaces, and elevators, among others. It is anticipated that CBNs will play an increasingly significant role in the fight against viruses and infectious diseases.

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Construction of a triple gene-deleted Chinese Pseudorabies virus variant and its efficacy study as a vaccine candidate on suckling piglets

Cited by 70 publications

References 14 publications

RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

RR1 and RR2 gene deletion affects the immunogenicity of a live attenuated pseudorabies virus vaccine candidate in natural pig host

Live attenuated pseudorabies virus developed using the CRISPR/Cas9 system

Carbon‐Based Nanomaterials for Antiviral Applications

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