Construction of an adenoviral expression vector carrying FLAG and hrGFP-1 genes and its expression in bone marrow mesenchymal stem cells

Wang, G.X.; Hu, Liang; Zhang, Z.; Liu, D.P.

doi:10.4238/2014.february.20.8

Cited by 4 publications

(2 citation statements)

References 19 publications

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“…To amplify the adenovirus, the acquired virus was used to infect HEK293A cells, and the cells were collected after 2 days. After amplification was repeated 3 times, all cells were collected and subjected to freezing and thawing repeatedly to liberate the recombinant adenovirus . pAd‐CMV empty vector and/or GFP were constructed in the same way for use as control.…”

Section: Methodsmentioning

confidence: 99%

Lin28a enhances in vitro osteoblastic differentiation of human periosteum‐derived cells

Park

Kang

et al. 2017

Cell Biochemistry & Function

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Despite a capacity for proliferation and an ability to differentiate into multiple cell types, in long-term culture and with ageing, stem cells show a reduction in growth, display a decrease in differentiation potential, and enter senescence without evidence of transformation. The Lin28a gene encodes an RNA-binding protein that plays a role in regulating stem cell activity, including self-renewal and differentiation propensity. However, the effect of the Lin28a gene on cultured human osteoprecursor cells is poorly understood. In the present study, alkaline phosphatase activity, alizarin red-positive mineralization, and calcium content, positive indicators of osteogenic differentiation, were significantly higher in cultured human periosteum-derived cells (hPDCs) with Lin28a overexpression compared with cells without Lin28a overexpression. Lin28a overexpression by hPDCs also increased mitochondrial activity, which is essential for cellular proliferation, as suggested by a reduced presence of reactive oxygen species and significantly enhanced lactate levels and ATP production. Our results suggest that, in hPDCs, the Lin28a gene enhances osteoblastic differentiation and increases mitochondrial activity. Although Lin28a is known as a marker of undifferentiated human embryogenic stem cell, there is limited evidence regarding the influence of Lin28a on osteoblastic differentiation of cultured osteoprecursor cells. This study was to examine the impact of Lin28a on osteogenic phenotypes of human periosteum-derived cells. Their phenotypes can be similar to those of mesenchymal stem cells. Our results suggest that the Lin28a gene enhances the osteoblastic differentiation of human periosteum-derived cells. In addition, the Lin28a gene increases mitochondrial activity in human periosteum-derived cells.

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Section: Methodsmentioning

confidence: 99%

Lin28a enhances in vitro osteoblastic differentiation of human periosteum‐derived cells

Park

Kang

et al. 2017

Cell Biochemistry & Function

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“…no. 1060) containing the green fluorescence protein (GFP) gene (Ad.GFP) was generated, purified, titrated, and stored as described previously (23).…”

Section: Methodsmentioning

confidence: 99%

LXR agonist T0901317 upregulates thrombomodulin expression in glomerular endothelial cells by inhibition of nuclear factor-κB

Ding

Feng

et al. 2016

Molecular Medicine Reports

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Dysfunction of glomerular endothelial cells (GECs) induces a variety of symptoms, including proteinuria, inflammation, vascular diseases, fibrosis and thrombosis. Thrombomodulin (TM) acts as a vasoprotective molecule on the surface of the vascular endothelial cells to maintain the homeostasis of the endothelial microenvironment by suppressing cellular proliferation, adhesion and inflammatory responses. Liver X receptor (LXR), a nuclear receptor (NR) and a bile acid‑activated transcription factor, regulates metabolism and cholesterol transport, vascular tension and inflammation. Previous studies indicated that TM expression is upregulated by various NRs; however, it is unclear whether pharmacological modulation of LXR may affect TM expression and GEC function. The current study revealed that LXR activation by its agonist, T0901317, upregulates the expression and activity of TM. This effect was mediated specifically through LXR‑α, and not through LXR‑β. Additionally, T0901317 treatment inhibited nuclear factor‑κB (NF‑κB) signaling and the secretion of high glucose‑induced proinflammatory mediators, including tumor necrosis factor‑α and interleukin‑1β in GECs. Co‑immunoprecipitation experiments determined that treatment with T0901317 enhances the interaction between LXR‑α and the transcriptional coactivator, p300, in GEC extracts. The present findings suggest that NF‑κB may be a negative regulator of TM expression, and its removal may contribute to TM gene expression, particularly when in competition with the T0901317‑enhanced formation of the LXR/p300 complex. Therefore, LXR may be a novel molecular target for manipulating TM in GECs, which may advance the treatment of endothelial cell‑associated diseases.

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SIRT3 and SIRT4 double-genes remodeled the mitochondrial network to induce hepatocellular carcinoma cell line differentiation and suppress malignant phenotypes

Zhang,

Dai,

Shi

et al. 2024

Biochemical Pharmacology

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Construction of an adenoviral expression vector carrying FLAG and hrGFP-1 genes and its expression in bone marrow mesenchymal stem cells

Cited by 4 publications

References 19 publications

Lin28a enhances in vitro osteoblastic differentiation of human periosteum‐derived cells

Lin28a enhances in vitro osteoblastic differentiation of human periosteum‐derived cells

LXR agonist T0901317 upregulates thrombomodulin expression in glomerular endothelial cells by inhibition of nuclear factor-κB

SIRT3 and SIRT4 double-genes remodeled the mitochondrial network to induce hepatocellular carcinoma cell line differentiation and suppress malignant phenotypes

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