Construction of an alkaline phosphatase–liposome system: a tool for biomineralization study

Camolezi, F L; Perez, Kátia Regina; Magalhães, Prislaine Pupolin; Pizauro, João Martins; Ciancaglini, Pietro

doi:10.1016/s1357-2725(02)00029-8

Cited by 68 publications

(99 citation statements)

References 48 publications

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“…the ordered DPPC-enriched membrane domains [67]. The minimal size of bright dots was compatible with structural data [56] and the time course of their visualization at the membrane surface agreed with previous work done on direct incorporation of AP-GPI into liposomes [15]. The presence of larger spots at the membrane surface also gave direct support for BIAP having a marked tendency to form clusters, a hypothesis formulated from indirect biochemical [13] and physicochemical [78] evidences.…”

Section: Afm Detection Of Ap-gpi In Model Membranessupporting

confidence: 87%

“…Most of the available biomimetic systems like LangmuirBlodgett films (LB), supported lipid bilayers (SLBs), large (LUVs), and giant unilamellar vesicles (GUVs) have been used to better characterize the formation of in-plane lipiddependent microdomains and lipid/AP-GPI interactions in membranes [15,17,21,52,55,67,78].…”

Section: Afm Detection Of Ap-gpi In Model Membranesmentioning

confidence: 99%

“…Osseous plate AP-GPI and PLAP can also spontaneously insert into preformed liposomes [15,54]. Compared with reconstitution procedures, where the protein is added before or during the bilayer formation, this process presents the important advantage to insure an exclusive right side outside orientation of the AP-GPI at the external leaflet of the membrane.…”

Section: Afm Detection Of Ap-gpi In Model Membranesmentioning

confidence: 99%

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Characterizing the interactions between GPI-anchored alkaline phosphatases and membrane domains by AFM

Giocondi

Seantier

Dosset

et al. 2007

Pflugers Arch - Eur J Physiol

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In plasma membranes, most glycosylphosphatidylinositol-anchored proteins (GPI proteins) would be associated with ordered microdomains enriched in sphingolipids and cholesterol. Debates on the composition and the nano-or mesoscales organization of these membrane domains are still opened. This complexity of biomembranes explains the use, in the recent years, of both model systems and atomic force microscopy (AFM) approaches to better characterize GPI proteins/membranes interactions. So far, the studies have mainly been focused on alkaline phosphatases of intestinal (BIAP) or placental (PLAP) origins reconstituted in model systems. The data show that GPI-anchored alkaline phosphatases (AP-GPI) molecules inserted in supported membranes can be easily imaged by AFM, in physiological buffer. They are generally observed in the most ordered domains of model membranes under phase separation, i.e. presenting both fluid and ordered domains. This direct access to the membrane structure at a mesoscopic scale allows establishing the GPI protein induced changes in microdomains size. It provides direct evidence for the temperature-dependent distribution of a GPI protein between fluid and ordered membrane domains. Origins of reported differences in the behavior of BIAP and PLAP are discussed. Finally, advantages and limits of AFM in the study of GPI proteins/membrane domains interactions are presented in this review.

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Section: Afm Detection Of Ap-gpi In Model Membranessupporting

confidence: 87%

Section: Afm Detection Of Ap-gpi In Model Membranesmentioning

confidence: 99%

Section: Afm Detection Of Ap-gpi In Model Membranesmentioning

confidence: 99%

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Characterizing the interactions between GPI-anchored alkaline phosphatases and membrane domains by AFM

Giocondi

Seantier

Dosset

et al. 2007

Pflugers Arch - Eur J Physiol

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“…To form unilamellar vesicles with a single concentric bilayer similar to natural membranes, multilamellar vesicles can be sonicated or extruded to nanometric dimensions. Sonication as well as extrusion processes supply the necessary energy to break the different lamellas and reorganize the bilayers into uniform and control-sized vesicles (Camolezi et al 2002;Daghastanli et al 2004;de Lima Santos et al 2005;Ierardi et al 2002;Rigos et al 2008;Simao et al 2015).…”

Section: What Are the Biomimetic Systems Of Mvs?mentioning

confidence: 99%

Biophysical aspects of biomineralization

et al. 2017

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During the process of endochondral bone formation, chondrocytes and osteoblasts mineralize their extracellular matrix (ECM) by promoting the synthesis of hydroxyapatite (HA) seed crystals in the sheltered interior of membranelimited matrix vesicles (MVs). Several lipid and proteins present in the membrane of the MVs mediate the interactions of MVs with the ECM and regulate the initial mineral deposition and posterior propagation. Among the proteins of MV membranes, ion transporters control the availability of phosphate and calcium needed for initial HA deposition. Phosphatases (orphan phosphatase 1, ectonucleotide pyrophosphatase/ phosphodiesterase 1 and tissue-nonspecific alkaline phosphatase) play a crucial role in controlling the inorganic pyrophosphate/inorganic phosphate ratio that allows MVmediated initiation of mineralization. The lipidic microenvironment can help in the nucleation process of first crystals and also plays a crucial physiological role in the function of MVassociated enzymes and transporters (type III sodiumdependent phosphate transporters, annexins and Na + /K + ATPase). The whole process is mediated and regulated by the action of several molecules and steps, which make the process complex and highly regulated. Liposomes and proteoliposomes, as models of biological membranes, facilitate the understanding of lipid-protein interactions with emphasis on the properties of physicochemical and biochemical processes. In this review, we discuss the use of proteoliposomes as multiple protein carrier systems intended to mimic the various functions of MVs during the initiation and propagation of mineral growth in the course of biomineralization. We focus on studies applying biophysical tools to characterize the biomimetic models in order to gain an understanding of the importance of lipid-protein and lipid-lipid interfaces throughout the process.Keywords Lipid microenvironment . Biomineralization . Matrix vesicles . Liposome . Proteoliposome . Hydroxyapatite Mineralization process and the biogenesis of matrix vesiclesMineralization of cartilage and bone occurs by a series of physicochemical and biochemical processes that together facilitate the deposition of calcium phosphate. The deposited calcium phosphate is subsequently converted into hydroxyapatite (HA) [Ca 10 (PO 4 ) 6 (OH) 2 ] in specific areas of the extracellular matrix (ECM). Various experiments have revealed the presence of HA crystals both inside and outside collagen fibrils in the ECM (McNally et al. 2012) and also within the

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“…For example, MLVs may be sonicated or passed through a membrane with pores of nanometric dimensions (extrusion method) to form unilamellar vesicles that have properties very similar those of to natural membranes. Both the sonication and extrusion methods provide the energy required to break the various lamellae that initially formed and reorganize the system into uniform bilayers with controlled sizes of vesicles (Camolezi et al 2002;Ierardi et al 2002;Daghastanli et al 2004;Santos et al 2002Santos et al , 2005Rigos et al 2008;dos Santos et al 2009;Bolean et al 2010Bolean et al , 2011Simão et al 2010a, b;Ciancaglini et al 2012).…”

Section: Liposomesmentioning

confidence: 99%

Liposomal systems as carriers for bioactive compounds

et al. 2015

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Since the revolutionary discovery that phospholipids can form closed bilayered structures in aqueous systems, the study of liposomes has become a very interesting area of research. The versatility and amazing biocompatibility of liposomes has resulted in their wide-spread use in many scientific fields, and many of their applications, especially in medicine, have yielded breakthroughs in recent decades. Specifically, their easy preparation and various structural aspects have given rise to broadly usable methodologies to internalize different compounds, with either lipophilic or hydrophilic properties. The study of compounds with potential biotechnological application(s) is generally related to evaluation and risk assessment of the possible cytotoxic or therapeutic effects of the compound under study. In most cases, undesirable sideeffects are associated with an interaction of the liposome with the cell membrane and/or its absorption and subsequent interaction with a cellular biomolecule. Liposomal carrier systems have an unprecedented potential for delivering bioactive substances to specific molecular targets due to their biocompatibility, biodegradability and low toxicity. Liposomes are therefore considered to be an invaluable asset in applied biotechnology studies due to their potential for interaction with both hydrophilic and lipophilic compounds.

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Construction of an alkaline phosphatase–liposome system: a tool for biomineralization study

Cited by 68 publications

References 48 publications

Characterizing the interactions between GPI-anchored alkaline phosphatases and membrane domains by AFM

Characterizing the interactions between GPI-anchored alkaline phosphatases and membrane domains by AFM

Biophysical aspects of biomineralization

Liposomal systems as carriers for bioactive compounds

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