Construction of an amperometric pyruvate oxidase enzyme electrode for determination of pyruvate and phosphate

Akyılmaz, Erol; Yorganci, Emine

doi:10.1016/j.electacta.2007.06.058

Cited by 40 publications

(17 citation statements)

References 24 publications

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“…[20] Amperometric biosensors are rapid and sensitive, but suffer from interference. [23] The mass spectrometric and enzymatic methods show good selectivity and sensitivity; both of them would be more promising if their relative high cost can be reduced. [20] Because of the improved spectroscopic properties and labeling conditions of the reagent RBPH, our proposed method may offer a new choice for the simple, selective, and sensitive determination of pyruvic acid.…”

Section: Linearitymentioning

confidence: 99%

“…Comparison with other methods: Several methods for determining pyruvic acid have been developed recently, including mass spectrometric methods, [22] amperometric biosensors based on pyruvate oxidase, [23] indirect amperometric capillary analysis, [24] and enzymatic fluorescence capillary analysis. [20] Amperometric biosensors are rapid and sensitive, but suffer from interference.…”

Section: Linearitymentioning

confidence: 99%

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Rhodamine B Piperazinoacetohydrazine: A Water‐Soluble Spectroscopic Reagent for Pyruvic Acid Labeling

Jia

Wang

Chen

et al. 2010

Chemistry A European J

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A new water-soluble reagent, rhodamine B piperazinoacetohydrazine (RBPH), with improved spectroscopic and reaction properties, has been developed and characterized for pyruvic acid labeling. The reagent RBPH is designed and synthesized by using rhodamine B as a spectroscopic unit, and hydrazine as a carbonyl-specific labeling unit; the two units are connected by a well-chosen linker of piperazine, which prohibits the formation of the nonfluorescent spirocyclic structure of rhodamine B, thereby keeping the spectroscopic response of the reagent in a stable state. Such a design enables RBPH not only to maintain its excellent spectroscopic properties over a wide pH range, but also to exist as a stable cation with high water solubility. Moreover, the hydrazino group of RBPH is expected to react selectively with carbonyl compounds under mild conditions through the rapid formation of hydrazones. These important features make RBPH of great potential use in the labeling of aldehydes or ketones in various biosystems, and such an application of RBPH as a precolumn derivatizing reagent has been successfully demonstrated on the analysis of pyruvic acid in human serum by high-performance liquid chromatography with common UV/Vis detection.

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Section: Linearitymentioning

confidence: 99%

Section: Linearitymentioning

confidence: 99%

Rhodamine B Piperazinoacetohydrazine: A Water‐Soluble Spectroscopic Reagent for Pyruvic Acid Labeling

Jia

Wang

Chen

et al. 2010

Chemistry A European J

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“…In the following decades, the immobilization of enzyme became a key issue in developing the enzyme-based biosensor. Generally speaking, the immobilized methods (Cunningham, 1998) of enzyme include adsorption (Chu et al, 2007;Ekanayake et al, 2007), entrapment (Ngounou et al, 2007;Seo et al, 2007), cross-linking (Akyilmaz & Yorganci, 2007) and covalent bonding (Lin et al, 2007a;Seo et al, 2007). For adsorption, the enzyme was attached on the electrode by the attractive force of hydrogen bonds or opposite charges, such as nylon (Gamati et al, 1991) and ion exchange resin (Zhujun & Seitz, 1986), but it did not form a good adhesive force between biomolecules and a transducer.…”

Section: The Immobilization Of Enzymes and Mediatorsmentioning

confidence: 99%

Amperometric Enzyme-based Biosensors for Lowering the Interferences

Nien¹,

Chen²,

Ho³

2010

Intelligent and Biosensors

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“…But most of these analytical techniques are often limited to low sensitivity, less improved detection limits, small sensitivity, negligible interference from real sample matrix, cost and/or fast response time for selective determination of phosphorous species [16][17][18][19][20][21][22][23][24][25][26][27][28]. Among these analytical techniques, CL can be considered as a promising detection system [15].…”

Section: Introductionmentioning

confidence: 99%

Aerosol Hygroscopic Growth as a New Factor for Trace and Ultra-Trace Determination of Phosphorous in Flame Containing Optical Trapping-Cavity Ring-Down Spectroscopy

Doroodmand¹,

Ghasemi²

2017

J Anal Bioanal Tech

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A new method has been introduced based on aerosol hygroscopic growth as a new factor for trace and ultra-trace determination of phosphorous in flame containing optical trapping-cavity ring down aerosol extinction (emission) spectrometer (OT-CRD-AES). In this study, a cavity ring down has been designed using hydrogen and air as fuel and oxidant during introduction of the aerosols containing phosphorous species using an ultrasonic generator (humidifier) from an acidic solution by a flow rate of N 2 , followed by detection of the Mie scattering using a charged coupling device (CCD) system. Parameters having strong influence during following scattering of the aerosols during their hygroscopic growth inside the humidified flame (H 2 /air), include: influence and amount of Na + as radiation buffer (as light source), flow rates of H 2 , air and N 2 , kind and concentrations of acid, evaluation of the aerosols inside flame, etc. These parameters were optimized using simplex and one at a time methods. Based on the figures of merit under optimized condition, two linear calibration curves with reverse slopes were evaluated between 10.0 -250.0 ng mL -1 and 1.0 -20.0 µg mL -1 with correlation coefficients (R 2 ) the same as 0.999 and 0.998, respectively. The calibration sensitivities were also estimated to 57.46 and 0.348 (a.u), respectively, with detection limit of 5.0 ng mL -1 . The mechanism of the radiation (Mie scattering) was also evidenced based on i) dependency of the scattered radiations to the quantity of an alkali ions such as Na+ as well as the humidity, ii) presence of acceptable correlation between the response of the cavity with turbidometry, iii) observation of blue shift from green (color related to the luminescence of HPO* in H 2 /air flame) to blue (scattered radiation) and finally iv) effect of hydration number during stability and growth of the aerosols inside the flames.No serious interference was evaluated during analysis of at least 500-fold excess of various foreign species. However, the only observed interference was evaluated during introduction of 200-fold excess of SO 4 2-. Good correlation was also evaluated between the results obtained from this technique and ion exchange chromatography during analysis of wastewater samples that clearly revealed the reliability of this method for phosphorous detection and determination at µg mL -1 and ng mL -1 levels.

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Construction of an amperometric pyruvate oxidase enzyme electrode for determination of pyruvate and phosphate

Cited by 40 publications

References 24 publications

Rhodamine B Piperazinoacetohydrazine: A Water‐Soluble Spectroscopic Reagent for Pyruvic Acid Labeling

Rhodamine B Piperazinoacetohydrazine: A Water‐Soluble Spectroscopic Reagent for Pyruvic Acid Labeling

Amperometric Enzyme-based Biosensors for Lowering the Interferences

Aerosol Hygroscopic Growth as a New Factor for Trace and Ultra-Trace Determination of Phosphorous in Flame Containing Optical Trapping-Cavity Ring-Down Spectroscopy

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