Construction of an inexpensive surface plasmon resonance instrument for use in teaching and research

Lavine, Barry K.; Westover, David J.; Oxenford, Leah; Mirjankar, Nikhil; Kaval, Necati

doi:10.1016/j.microc.2007.02.003

Cited by 16 publications

(8 citation statements)

References 26 publications

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“…In figure 2, this defines a point on the SP dispersion relation. The incidence angle of this laser beam θ int has to be adjusted so that the light line given by relation (9) goes through this point. This angle is a solution of equation (10).…”

Section: Excitation Of the Sp Wavementioning

confidence: 99%

Laboratory experiments for exploring the surface plasmon resonance

Pluchery¹,

Vayron

Van

2011

Eur. J. Phys.

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The surface plasmon wave is a surface wave confined at the interface between a dielectric and a metal. The excitation of the surface plasmon resonance (SPR) on a gold thin film is discussed within the Kretschmann configuration, where the coupling with the excitation light is achieved by means of a prism in total reflection. The electromagnetic principles are detailed and a simple experimental setup is described that can be used for laboratory experiments for senior students in the third or fourth year of university. This experiment allows accurate determination of the angle of plasmon extinction and discussion of the principles of biosensors based on the SPR. A slight modification of the setup allows the investigation of the dependence of SPR on wavelength and illustrates the damping of SPR due to its coupling with the interband transitions of the gold thin film.

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Section: Excitation Of the Sp Wavementioning

confidence: 99%

Laboratory experiments for exploring the surface plasmon resonance

Pluchery¹,

Vayron

Van

2011

Eur. J. Phys.

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“…Students observe that the presence of the enhancement effect can be directly correlated with the presence of the experimental variables responsible for the effect: an electronic transition overlapping the excitation frequency of the laser. The relative intensities of the peaks in each spectrum change because the degree to which each mode is coupled to the Franck–Condon transition is different. , While an in-depth description of the quantum-mechanical interactions responsible is likely beyond the level of the students targeted in this work, excellent discussions can be found in the literature. ,− Our experiment provides students with direct, hands-on control over the relevant experimental variables, combating the tendency among some students to perceive spectroscopic instrumentation as a “black box” into which samples are deposited to await mysterious analysis. − This can also be an opportunity for students to discover the applications of this enhancement mechanism for structural elucidation or to develop more sensitive measurement techniques.…”

Section: Discussionmentioning

confidence: 99%

Exploring Resonance Raman Scattering with 4-Nitrophenol

et al. 2022

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Raman scattering is a powerful tool for revealing the vibrations of molecules, but as a nonlinear optical phenomenon, its signals can change via mechanisms like resonance enhancement that have no direct analogue in infrared spectroscopy. In this work, complementary measurements conducted on 4-nitrophenol and its conjugate base allow students to directly control the variables responsible for enhancement. Changing sample UV–vis absorbance with pH and excitation color with the addition of a diode laser allows students to explore the criteria for resonance Raman scattering: only the conjugate base and 405 nm laser in combination produce enhancement. Adjustments to the experiment make it suitable for either an upper-level undergraduate course lab or an independent inquiry project.

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“…The homebuilt SPR instrument and the sample flow cell used in this study have been described elsewhere. 6 Slides for SPR were prepared from a 3″×1″×1/16″ SF10 slide (ESCO, NJ). The slide was cleaned using a piranha solution (one part 30% hydrogen peroxide and three parts concentrated sulfuric acid; obtained from Fisher Scientific; Warning: Piranah solution is highly reactive; handle with appropriate precautions ).…”

Section: Methodsmentioning

confidence: 99%

Characterization of Swellable Molecularly Imprinted Polymer Particles by Surface Plasmon Resonance Spectroscopy

et al. 2012

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Surface plasmon resonance (SPR) has been used to investigate template binding at sites in theophylline-imprinted poly-[N-(N-propyl) acrylamide] particles. At concentrations as low as 10(-6) M theophylline, particle swelling is detected as a shift in the angle of minimum reflectance. The binding constant of theophylline estimated from the inflection point of the theophylline calibration curve is approximately 10,000. The imprinted polymer particles do not respond to caffeine or theobromine (which differs from theophylline by a single methyl group) at concentrations as high as 10(-2) M. Full-scale response of the imprinted polymer particles to theophylline (template) occurs in less than 15 minutes, and swelling is reversible. The immobilized imprinted polymer particles can undergo approximately 20 to 25 swelling and shrinking cycles before there is significant loss in functionality. A unique aspect of these imprinted polymer particles is that template binding causes the angle of minimum reflectance to decrease, not increase, in magnitude. Adsorption, which causes an increase in the angle of minimum reflectance, can be readily discriminated from template binding.

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Construction of an inexpensive surface plasmon resonance instrument for use in teaching and research

Cited by 16 publications

References 26 publications

Laboratory experiments for exploring the surface plasmon resonance

Laboratory experiments for exploring the surface plasmon resonance

Exploring Resonance Raman Scattering with 4-Nitrophenol

Characterization of Swellable Molecularly Imprinted Polymer Particles by Surface Plasmon Resonance Spectroscopy

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