Construction of an Infectious Clone for Mosquito-Derived Tembusu Virus Prototypical Strain

Wang, Xiaoli; He, Ying; Guo, Jiaqi; Jiang, Bowen; Wang, Mingshu; Jia, Renyong; Zhu, Dekang; Liu, Mafeng; Zhao, Xinxin; Yang, Qiao; Wu, Ying; Zhang, Shaqiu; Liu, Yunya; Yu, Yanling; Cheng, Anchun; Chen, Shun

doi:10.1007/s12250-021-00447-y

Cited by 4 publications

(10 citation statements)

References 10 publications

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“…Host-specific adaptation is one of the necessary steps for viral host switching. In the present study, we first verified that the duck-origin strain rCQW1 exhibited higher cellular fitness in DEF cells and BHK-21 cells than the mosquito-origin strain rMM 1775, and the results were the same as those previously reported ( 24 ). Then, we further found that duck-derived CQW1 3'UTR could improve the proliferation ability of mosquito-derived rMM 1775 in BHK-21 and DEF cells.…”

Section: Discussionsupporting

confidence: 88%

“…2D and E ). Moreover, our previous study indicated that rMM 1775 showed stronger replication ability than rCQW1 in C6/36 cells ( 24 ). Altogether, these data suggested that TMUV rCQW1 and rMM 1775 exhibited significant proliferation differences in vitro ; rCQW1 exhibited higher viral titers and NS3 expression levels in both BHK-21 and DEF cells and stronger infectivity in BHK-21 cells but weaker proliferation in C6/36 cells.…”

Section: Resultsmentioning

confidence: 98%

“…In our previous study, we successfully rescued a duck-derived TMUV rCQW1 and a mosquito-derived TMUV rMM 1775 by reverse genetics technology ( 23 , 24 ). First, we compared the viral proliferation characteristics of these two strains in vitro .…”

Section: Resultsmentioning

confidence: 99%

“…The duck-derived TMUV strain CQW1 (GenBank ID KM233707.1 ) was rescued from a DNA-based full-length infectious clone reported previously ( 53 ). The mosquito-derived TMUV strain MM 1775 (GenBank ID JX477685.2 ) was collected as mentioned above ( 24 ). All virus stocks were amplified in BHK-21 cells.…”

Section: Methodsmentioning

confidence: 99%

“…In previous studies, we observed that the mosquito-origin TMUV rMM 1775 and the duck-origin TMUV rCQW1 exhibited significant differences in multiplication in vitro ( 23 , 24 ). To further identify the role that the TMUV 3′UTR played in proliferation in vitro and the pathogenicity of the virus for mice, a series of chimeric viruses with heterogeneous 3′UTRs were generated based on the reverse genetic method using CQW1 or MM 1775 as the backbone.…”

Section: Introductionmentioning

confidence: 91%

See 4 more Smart Citations

Stem-Loop I of the Tembusu Virus 3′-Untranslated Region Is Responsible for Viral Host-Specific Adaptation and the Pathogenicity of the Virus in Mice

et al. 2022

Microbiol Spectr

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show abstract

Section: Discussionsupporting

confidence: 88%

Section: Resultsmentioning

confidence: 98%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

See 3 more Smart Citations

Stem-Loop I of the Tembusu Virus 3′-Untranslated Region Is Responsible for Viral Host-Specific Adaptation and the Pathogenicity of the Virus in Mice

et al. 2022

Microbiol Spectr

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Comparative study of the pathogenicity of the mosquito origin strain and duck origin strain of Tembusu virus in ducklings and three-week-old mice

Wang

Guo

et al. 2023

Virologica Sinica

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Vector competence of Culex quinquefasciatus for Tembusu virus and viral factors for virus transmission by mosquitoes

Tang,

He,

Wang

et al. 2024

Vet Res

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The ongoing epidemic of flaviviruses worldwide has underscored the importance of studying flavivirus vector competence, considering their close association with mosquito vectors. Tembusu virus is an avian-related mosquito-borne flavivirus that has been an epidemic in China and Southeast Asia since 2010. However, the reason for the outbreak of Tembusu virus in 2010 remains unclear, and it is unknown whether changes in vector transmission played an essential role in this process. To address these questions, we conducted a study using Culex quinquefasciatus as a model for Tembusu virus infection, employing both oral infection and microinjection methods. Our findings confirmed that both vertical and venereal transmission collectively contribute to the cycle of Tembusu virus within the mosquito population, with persistent infections observed. Importantly, our data revealed that the prototypical Tembusu virus MM_1775 strain exhibited significantly greater infectivity and transmission rates in mosquitoes than did the duck Tembusu virus (CQW1 strain). Furthermore, we revealed that the viral E protein and 3′ untranslated region are key elements responsible for these differences. In conclusion, our study sheds light on mosquito transmission of Tembusu virus and provides valuable insights into the factors influencing its infectivity and transmission rates. These findings contribute to a better understanding of Tembusu virus epidemiology and can potentially aid in the development of strategies to control its spread.

show abstract

Construction of an Infectious Clone for Mosquito-Derived Tembusu Virus Prototypical Strain

Cited by 4 publications

References 10 publications

Stem-Loop I of the Tembusu Virus 3′-Untranslated Region Is Responsible for Viral Host-Specific Adaptation and the Pathogenicity of the Virus in Mice

Stem-Loop I of the Tembusu Virus 3′-Untranslated Region Is Responsible for Viral Host-Specific Adaptation and the Pathogenicity of the Virus in Mice

Comparative study of the pathogenicity of the mosquito origin strain and duck origin strain of Tembusu virus in ducklings and three-week-old mice

Vector competence of Culex quinquefasciatus for Tembusu virus and viral factors for virus transmission by mosquitoes

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