Construction of an ultrasensitive electrochemical sensing platform for microRNA-21 based on interface impedance spectroscopy

Meng, Tianjiao; Zhao, Dan; Ye, Huimin; Feng, Yue; Wang, Huan; Zhang, Yufan

doi:10.1016/j.jcis.2020.05.118

Cited by 51 publications

(12 citation statements)

References 50 publications

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“…Association of this method with system amplification such as HCR was also performed. Indeed, Meng et al [170] developed an electrochemical biosensor combining efficient HCR for signal amplification of oligonucleotides with negatively charged repelling [Fe(CN) 6 ] 3−/4− ions inducing a spatial blockage to the electron transfer. In this biosensor, many linear DNA concatamers lead to a great increase in interfacial charge-transfer resistance (R ct ), which is positively correlated with miRNA-21 concentrations with an LOD of 4.63 fM with the stability of the biosensor lasting for 21 days.…”

Section: Ferri/ferrocyanide As Free Redox Indicatormentioning

confidence: 99%

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

et al. 2020

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Cancer is the second most fatal disease in the world and an early diagnosis is important for a successful treatment. Thus, it is necessary to develop fast, sensitive, simple, and inexpensive analytical tools for cancer biomarker detection. MicroRNA (miRNA) is an RNA cancer biomarker where the expression level in body fluid is strongly correlated to cancer. Various biosensors involving the detection of miRNA for cancer diagnosis were developed. The present review offers a comprehensive overview of the recent developments in electrochemical biosensor for miRNA cancer marker detection from 2015 to 2020. The review focuses on the approaches to direct miRNA detection based on the electrochemical signal. It includes a RedOx-labeled probe with different designs, RedOx DNA-intercalating agents, various kinds of RedOx catalysts used to produce a signal response, and finally a free RedOx indicator. Furthermore, the advantages and drawbacks of these approaches are highlighted.

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Section: Ferri/ferrocyanide As Free Redox Indicatormentioning

confidence: 99%

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

et al. 2020

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“…Unfortunately, current work mainly focuses on improving one part of them but ignores the importance of studying the four aspects as a whole [ 24 , 25 , 26 ]. Since the electrode interface, recognition element and signal outputs are the unified whole of electrochemical sensors, it is difficult to achieve desired detection performance by only enhancing one part, either developing new electrodes or constructing a detection mode [ 27 , 28 , 29 ]. That is, only by considering mutual influences from different aspects, can it be possible to construct stable and reliable electrochemical sensing methods.…”

Section: Introductionmentioning

confidence: 99%

Towards Development of Molecularly Imprinted Electrochemical Sensors for Food and Drug Safety: Progress and Trends

et al. 2022

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Due to their advantages of good flexibility, low cost, simple operations, and small equipment size, electrochemical sensors have been commonly employed in food safety. However, when they are applied to detect various food or drug samples, their stability and specificity can be greatly influenced by the complex matrix. By combining electrochemical sensors with molecular imprinting techniques (MIT), they will be endowed with new functions of specific recognition and separation, which make them powerful tools in analytical fields. MIT-based electrochemical sensors (MIECs) require preparing or modifying molecularly imprinted polymers (MIPs) on the electrode surface. In this review, we explored different MIECs regarding the design, working principle and functions. Additionally, the applications of MIECs in food and drug safety were discussed, as well as the challenges and prospects for developing new electrochemical methods. The strengths and weaknesses of MIECs including low stability and electrode fouling are discussed to indicate the research direction for future electrochemical sensors.

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“…12 It includes voltammetry, amperometry and impedance spectroscopy, in which electrochemical impedance spectroscopy (EIS) is used to convert biological signals into measurable impedance signals for the determination of biomolecules in the biological field. [13][14][15] Therefore, we can develop a rapid, sensitive and low-cost label-free electrochemical impedance biosensor for the detection of CYFRA 21-1. 16 However, due to the extremely low concentration of CYFRA 21-1 in normal tissue and the early stage of lung cancer patients, improving the sensitivity of biosensor detection is the focus of current research.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical impedance analysis of the CYFRA 21-1 antigen based on doxorubicin-initiated ROP signal amplification

et al. 2022

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Construction of an ultrasensitive electrochemical sensing platform for microRNA-21 based on interface impedance spectroscopy

Cited by 51 publications

References 50 publications

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

Electrochemical Biosensors for Detection of MicroRNA as a Cancer Biomarker: Pros and Cons

Towards Development of Molecularly Imprinted Electrochemical Sensors for Food and Drug Safety: Progress and Trends

Electrochemical impedance analysis of the CYFRA 21-1 antigen based on doxorubicin-initiated ROP signal amplification

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