Construction of anti-adhesive and antibacterial multilayer films via layer-by-layer assembly of heparin and chitosan

Fu, Jinhong; Ji, Jian; Yuan, Weiyong; Shen, Jiacong

doi:10.1016/j.biomaterials.2005.04.034

Cited by 438 publications

(390 citation statements)

References 19 publications

Supporting

Mentioning

356

Contrasting

Unclassified

Order By: Relevance

“…21 Consequently, there is less restriction to the underlying substrate chemistry such as defects and trace contaminations, and moreover, numerous charged biomolecules including polysaccharides, 13,23 polypeptides, and proteins 24,25 can be used to produce surfaces tailored specifically for a variety of applications. Here, positively charged poly-L-lysine (PLL) which, at physiological conditions, is well known as a promoter of cell adhesion, 26,27 and negatively charged heparin that has anti-inflammatory 28,29 and anti-adhesive 30 properties along with its specific binding of growth factors, 31,32 were used. These deposited PEMs also provide abundant adsorption sites or reactive moieties for growth factor incorporation, 23 such as brain-derived neurotrophic factor (BDNF) which we exploited in this study.…”

Section: Introductionmentioning

confidence: 99%

Optimizing interfacial features to regulate neural progenitor cells using polyelectrolyte multilayers and brain derived neurotrophic factor

et al. 2011

View full text Add to dashboard Cite

The development of biomaterials with controllable interfacial features which have the capability to instruct cellular behavior are required to produce functional scaffolds for the treatment of spinal cord injury (SCI). Here, poly-e-caprolactone surfaces were biofunctionalized via layer-by-layer (LbL) deposition. The polyelectrolytes employed in this LbL technique were heparin and poly-L-lysine (PLL), the latter being chosen to improve cell adhesion and the subsequent cellular function of in vitrocultured neural progenitor cells. Material characterization results confirmed the deposition of well structured multilayers. Cell culture studies revealed significant differences in the cellular response to these adhesive/nonadhesive (PLL/heparin) polyelectrolyte multilayer (PEM)surfaces, with neurite outgrowth being significantly promoted on the PLL terminating layers. In addition, brain derived neurotrophic factor (BDNF) was adsorbed onto the LbL surfaces. This combined chemical and biological effect was then characterized in terms of neurite length along with the full length/truncated isoform 1 tyrosine kinase receptor (TrkB-FL/TrkB-T1) and growth associated protein-43 mRNA levels. Here, the authors report the differential effect of adsorbed and soluble BDNF of different concentrations. Adsorbed BDNF promoted neurite outgrowth and led to elevated, sustained TrkB mRNA levels. These findings highlight the potential of PEM biofunctionalized surfaces with integrated chemical and neurotrophin supportive cues to overcome SCI inhibitory environments and to promote

show abstract

Section: Introductionmentioning

confidence: 99%

Optimizing interfacial features to regulate neural progenitor cells using polyelectrolyte multilayers and brain derived neurotrophic factor

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Examples of active antibacterial coatings include cationic polymers such as chitosan (Huh et al, 2001;Fu et al, 2005) and polymers containing quaternary ammonium (Lee et al, 2004;Cheng et al, 2005;Murata et al, 2007) and pyridinium (Tiller et al, 2001;Tiller et al, 2002;Cen et al, 2004;Krishnan et al, 2006) functional groups. The antibacterial effect of silver can be exploited by incorporation of silver salts or silver nanoparticles into coatings (Sambhy et al, 2006;Marini et al, 2007;Ramstedt et al, 2007a;Ramstedt et al, 2007b).…”

Section: Introductionmentioning

confidence: 99%

Surface-immobilised antimicrobial peptoids

et al. 2008

View full text Add to dashboard Cite

Surface modification techniques that create surfaces capable of killing adherent bacteria are promising solutions to infections associated with implantable medical devices. Antimicrobial peptoid oligomers (ampetoids) that were designed to mimic helical antimicrobial peptides were synthesized with a peptoid spacer chain to allow mobility and an adhesive peptide moiety for easy and robust immobilization onto substrates. TiO 2 substrates were modified with the ampetoids and subsequently backfilled with an antifouling polypeptoid polymer in order to create polymer surface coatings composed of both antimicrobial (active) and antifouling (passive) peptoid functionalities. Confocal microscopy images show that the membranes of adherent E. coli were damaged after 2 h exposure to the modified substrates, suggesting that ampetoids retain antimicrobial properties even when immobilized onto substrates.

show abstract

“…CHI-containing multilayer nanofilms showed high resistance to bacterial adhesion and led to a substantial decrease in Escherichia coli adhesion compared to control bare substrates. 7,14,15 Moreover, CHIcontaining multilayer nanofilms prepared at different ionic strengths showed different bacterial resistance properties, 7 and the LbL process solution pH had a remarkable effect on the antibacterial properties of polyelectrolyte multilayer nanofilms.…”

Section: Applications Of Polyelectrolyte Multilayer Nanofilms As Tunamentioning

confidence: 99%

Advances in polyelectrolyte multilayer nanofilms as tunable drug delivery systems

Jiang

Barnett²,

2009

NSA

View full text Add to dashboard Cite

Construction of anti-adhesive and antibacterial multilayer films via layer-by-layer assembly of heparin and chitosan

Cited by 438 publications

References 19 publications

Optimizing interfacial features to regulate neural progenitor cells using polyelectrolyte multilayers and brain derived neurotrophic factor

Optimizing interfacial features to regulate neural progenitor cells using polyelectrolyte multilayers and brain derived neurotrophic factor

Surface-immobilised antimicrobial peptoids

Advances in polyelectrolyte multilayer nanofilms as tunable drug delivery systems

Contact Info

Product

Resources

About