Construction of BAC libraries from two apomictic grasses to study the microcolinearity of their apospory-specific genomic regions

Roche, Dominique; Conner, Joann A.; Budiman, Muhammad A.; Frisch, David; Wing, Rod A.; Hanna, W. W.; Ozias‐Akins, Peggy

doi:10.1007/s00122-001-0795-y

Cited by 37 publications

(6 citation statements)

References 35 publications

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“…Enzymatic maceration of root tips and anthers for chromosome spreads and denaturation for probe hybridization were conducted using the indicated times for each species (Table 2). ASGR-linked bacterial artificial chromosome (BAC) clones (P001 - containing SCAR A14M; P109 - SCAR Q8M; P208 - SCAR UGT197) have been described previously [35,38,64]. A BAC (P602) containing SCAR marker X18 and a large amount of repetitive DNA was isolated by PCR screening of pooled BAC DNAs [36].…”

Section: Methodsmentioning

confidence: 99%

Evolution of the apomixis transmitting chromosome in Pennisetum

et al. 2011

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BackgroundApomixis is an intriguing trait in plants that results in maternal clones through seed reproduction. Apomixis is an elusive, but potentially revolutionary, trait for plant breeding and hybrid seed production. Recent studies arguing that apomicts are not evolutionary dead ends have generated further interest in the evolution of asexual flowering plants.ResultsIn the present study, we investigate karyotypic variation in a single chromosome responsible for transmitting apomixis, the Apospory-Specific Genomic Region carrier chromosome, in relation to species phylogeny in the genera Pennisetum and Cenchrus. A 1 kb region from the 3' end of the ndhF gene and a 900 bp region from trnL-F were sequenced from 12 apomictic and eight sexual species in the genus Pennisetum and allied genus Cenchrus. An 800 bp region from the Apospory-Specific Genomic Region also was sequenced from the 12 apomicts. Molecular cytological analysis was conducted in sixteen Pennisetum and two Cenchrus species. Our results indicate that the Apospory-Specific Genomic Region is shared by all apomictic species while it is absent from all sexual species or cytotypes. Contrary to our previous observations in Pennisetum squamulatum and Cenchrus ciliaris, retrotransposon sequences of the Opie-2-like family were not closely associated with the Apospory-Specific Genomic Region in all apomictic species, suggesting that they may have been accumulated after the Apospory-Specific Genomic Region originated.ConclusionsGiven that phylogenetic analysis merged Cenchrus and newly investigated Pennisetum species into a single clade containing a terminal cluster of Cenchrus apomicts, the presumed monophyletic origin of Cenchrus is supported. The Apospory-Specific Genomic Region likely preceded speciation in Cenchrus and its lateral transfer through hybridization and subsequent chromosome repatterning may have contributed to further speciation in the two genera.

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Section: Methodsmentioning

confidence: 99%

Evolution of the apomixis transmitting chromosome in Pennisetum

et al. 2011

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“…Moreover, loss of pairing after local chromosome rearrangement was also reported in other apomictic species such as Pennisetum squamulatum (Ozias-Akins et al, 1998) and Cenchrus ciliaris (Goel et al, 2003). However, the non-recombining ACR of Paspalum appears relatively modest in size if compared with other models (Roche et al, 2002) as single apomixis-linked bacterial artificial chromosome (BAC) clones were identified by multiple markers that were independently developed (Calderini et al, 2011). Fine structure of the ACR inferred from sequencing of an apomixis-related BAC and of molecular markers completely linked to the trait…”

Section: Molecular Dissection Of the Apomixis-controlling Regionmentioning

confidence: 99%

Harnessing apomictic reproduction in grasses: what we have learned from Paspalum

et al. 2013

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In this article, the available literature characterizing apomixis in Paspalum spp. and its use in breeding is critically reviewed. In particular, a comparison is made across species of the structure and function of the genomic region controlling apomixis in order to identify a common core region shared by all apomictic Paspalum species and where apomixis genes are likely to be localized. Candidate genes are discussed, either as possible genetic determinants (including homologs to signal transduction and RNA methylation genes) or as downstream factors (such as cell-to-cell signalling and auxin response genes) depending, respectively, on their co-segregation with apomixis or less. Strategies to validate the role of candidate genes in apomictic process are also discussed, with special emphasis on plant transformation in natural apomictic species.

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“…BACs linked to the ASGR-carrier chromosome were identified by screening the polyhaploid BAC library [ 16 ] with probes derived from the ASGR-carrier chromosome transcripts [ 13 ]. Hybridizing BAC clones were confirmed as linked to the ASGR-carrier chromosome via PCR amplification with the respective ASGR-carrier chromosome SCAR marker.…”

Section: Methodsmentioning

confidence: 99%

In Silico and Fluorescence In Situ Hybridization Mapping Reveals Collinearity between the Pennisetum squamulatum Apomixis Carrier-Chromosome and Chromosome 2 of Sorghum and Foxtail Millet

et al. 2016

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Apomixis, or clonal propagation through seed, is a trait identified within multiple species of the grass family (Poaceae). The genetic locus controlling apomixis in Pennisetum squamulatum (syn Cenchrus squamulatus) and Cenchrus ciliaris (syn Pennisetum ciliare, buffelgrass) is the apospory-specific genomic region (ASGR). Previously, the ASGR was shown to be highly conserved but inverted in marker order between P. squamulatum and C. ciliaris based on fluorescence in situ hybridization (FISH) and varied in both karyotype and position of the ASGR on the ASGR-carrier chromosome among other apomictic Cenchrus/Pennisetum species. Using in silico transcript mapping and verification of physical positions of some of the transcripts via FISH, we discovered that the ASGR-carrier chromosome from P. squamulatum is collinear with chromosome 2 of foxtail millet and sorghum outside of the ASGR. The in silico ordering of the ASGR-carrier chromosome markers, previously unmapped in P. squamulatum, allowed for the identification of a backcross line with structural changes to the P. squamulatum ASGR-carrier chromosome derived from gamma irradiated pollen.

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Construction of BAC libraries from two apomictic grasses to study the microcolinearity of their apospory-specific genomic regions

Cited by 37 publications

References 35 publications

Evolution of the apomixis transmitting chromosome in Pennisetum

Evolution of the apomixis transmitting chromosome in Pennisetum

Harnessing apomictic reproduction in grasses: what we have learned from Paspalum

In Silico and Fluorescence In Situ Hybridization Mapping Reveals Collinearity between the Pennisetum squamulatum Apomixis Carrier-Chromosome and Chromosome 2 of Sorghum and Foxtail Millet

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