Construction of conductive network using magnetite to enhance microflora interaction and petroleum hydrocarbons removal in plant-rhizosphere microbial electrochemical system

Zhang, Xiaolin; Li, Ruixiang; Wang, Jinning; Liao, Chengmei; Zhou, Lean; An, Jingkun; Li, Tian; Wang, Xin; Zhou, Qixing

doi:10.1016/j.cej.2021.133600

Cited by 12 publications

(8 citation statements)

References 62 publications

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“…Less abundant Firmicutes in these experiments can be explained by the loss of Firmicutes ’ advantage in competition with Proteobacteria . Decreases in Firmicutes under such conditions was also observed in previous studies . Along with changes in the abundance of specific phyla, the total number of microbial species also changed in response to the addition of ferrihydrite or electroactive microbes.…”

Section: Resultssupporting

confidence: 81%

“…So, the electroactive bacteria performing dissimilatory iron reduction to synthesize magnetite show the potential in strengthening DIET in soil ecosystems. Bioproduction of magnetite by either Geobacter or Clostridia , Anaerosolibacter , and Thermincola shown in our soil systems has been demonstrated. , As shown in a previous study on bioelectrochemistry, the combination of exogenous magnetite with electrodes builds an extracellular electron transfer network in soil, strengthening the direct interspecific electron transfer and long-distance electron transport.…”

Section: Resultssupporting

confidence: 77%

“…Decreases in Firmicutes under such conditions was also observed in previous studies. 45 Along with changes in the abundance of specific phyla, the total number of microbial species also changed in response to the addition of ferrihydrite or electroactive microbes. In the experiment with soil only (S), the total number of species was 739, and this number decreased to 716 in the experiment with both ferrihydrite and electroactive microbes (S+Fe+B) and further decreased to 466 in the experiment with exogenous electroactive microbes (S+B).…”

Section: Enhanced Fe(iii) Mineral Reduction By Electroactivementioning

confidence: 99%

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Exogenous Electroactive Microbes Regulate Soil Geochemical Properties and Microbial Communities by Enhancing the Reduction and Transformation of Fe(III) Minerals

Zhang,

Liu,

Zhou

et al. 2023

Environ. Sci. Technol.

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Electroactive microbes can conduct extracellular electron transfer and have the potential to be applied as a bioresource to regulate soil geochemical properties and microbial communities. In this study, we incubated Fe-limited and Fe-enriched farmland soil together with electroactive microbes for 30 days; both soils were incubated with electroactive microbes and a common iron mineral, ferrihydrite. Our results indicated that the exogenous electroactive microbes decreased soil pH, total organic carbon (TOC), and total nitrogen (TN) but increased soil conductivity and promoted Fe(III) reduction. The addition of electroactive microbes also changed the soil microbial community from Firmicutes-dominated to Proteobacteria-dominated. Moreover, the total number of detected microbial species in the soil decreased from over 700 to less than 500. Importantly, the coexistence of N-transforming bacteria, Fe(III)-reducing bacteria and methanogens was also observed with the addition of electroactive microbes in Fe-rich soil, indicating the accelerated interspecies electron transfer of functional microflora.

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Section: Resultssupporting

confidence: 81%

Section: Resultssupporting

confidence: 77%

Section: Enhanced Fe(iii) Mineral Reduction By Electroactivementioning

confidence: 99%

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Exogenous Electroactive Microbes Regulate Soil Geochemical Properties and Microbial Communities by Enhancing the Reduction and Transformation of Fe(III) Minerals

Zhang,

Liu,

Zhou

et al. 2023

Environ. Sci. Technol.

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“…Depending on the treatment, electrically conductive particles of biochar or magnetite (5% w / w ) were added to the bottles. The amount of electrically conductive particles added to the microcosms (5% w / w ) was comparable with previous studies reported in the literature [ 5 , 28 , 29 , 30 , 31 , 32 ]. After preparation, Teflon-coated butyl stoppers and aluminium seals were used to seal all the bottles, which were then purged with nitrogen (0.5 bar for 20 min) to create anaerobic conditions.…”

Section: Methodssupporting

confidence: 88%

Enhancing the Anaerobic Biodegradation of Petroleum Hydrocarbons in Soils with Electrically Conductive Materials

et al. 2023

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Anaerobic bioremediation is a relevant process in the management of sites contaminated by petroleum hydrocarbons. Recently, interspecies electron transfer processes mediated by conductive minerals or particles have been proposed as mechanisms through which microbial species within a community share reducing equivalents to drive the syntrophic degradation of organic substrates, including hydrocarbons. Here, a microcosm study was set up to investigate the effect of different electrically conductive materials (ECMs) in enhancing the anaerobic biodegradation of hydrocarbons in historically contaminated soil. The results of a comprehensive suite of chemical and microbiological analyses evidenced that supplementing the soil with (5% w/w) magnetite nanoparticles or biochar particles is an effective strategy to accelerate the removal of selected hydrocarbons. In particular, in microcosms supplemented with ECMs, the removal of total petroleum hydrocarbons was enhanced by up to 50% relative to unamended controls. However, chemical analyses suggested that only a partial bioconversion of contaminants occurred and that longer treatment times would have probably been required to drive the biodegradation process to completion. On the other hand, biomolecular analyses confirmed the presence of several microorganisms and functional genes likely involved in hydrocarbon degradation. Furthermore, the selective enrichment of known electroactive bacteria (i.e., Geobacter and Geothrix) in microcosms amended with ECMs, clearly pointed to a possible role of DIET (Diet Interspecies Electron Transfer) processes in the observed removal of contaminants.

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“…Previous studies showed that the bioelectricity generated by soil MFCs could stimulate the growth of functional microbes, thus promoting the degradation of pollutants [13,27,28]. This is mainly because the metabolism of organic matter is accelerated through electron transfer, hence it is necessary to study the electron transfer process in soil MFCs.…”

Section: Introductionmentioning

confidence: 99%

Key genes of electron transfer, the nitrogen cycle and tetracycline removal in bioelectrochemical systems

Zhao,

Qin,

Jing

et al. 2023

Biotechnol Biofuels

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Background Soil microbial fuel cells (MFCs) can remove antibiotics and antibiotic resistance genes (ARGs) simultaneously, but their removal mechanism is unclear. In this study, metagenomic analysis was employed to reveal the functional genes involved in degradation, electron transfer and the nitrogen cycle in the soil MFC. Results The results showed that the soil MFC effectively removed tetracycline in the overlapping area of the cathode and anode, which was 64% higher than that of the control. The ARGs abundance increased by 14% after tetracycline was added (54% of the amplified ARGs belonged to efflux pump genes), while the abundance decreased by 17% in the soil MFC. Five potential degraders of tetracycline were identified, especially the species Phenylobacterium zucineum, which could secrete the 4-hydroxyacetophenone monooxygenase encoded by EC 1.14.13.84 to catalyse deacylation or decarboxylation. Bacillus, Geobacter, Anaerolinea, Gemmatirosa kalamazoonesis and Steroidobacter denitrificans since ubiquinone reductase (encoded by EC 1.6.5.3), succinate dehydrogenase (EC 1.3.5.1), Coenzyme Q-cytochrome c reductase (EC 1.10.2.2), cytochrome-c oxidase (EC 1.9.3.1) and electron transfer flavoprotein-ubiquinone oxidoreductase (EC 1.5.5.1) served as complexes I, II, III, IV and ubiquinone, respectively, to accelerate electron transfer. Additionally, nitrogen metabolism-related gene abundance increased by 16% to support the microbial efficacy in the soil MFC, and especially EC 1.7.5.1, and coding the mutual conversion between nitrite and nitrate was obviously improved. Conclusions The soil MFC promoted functional bacterial growth, increased functional gene abundance (including nitrogen cycling, electron transfer, and biodegradation), and facilitated antibiotic and ARG removal. Therefore, soil MFCs have expansive prospects in the remediation of antibiotic-contaminated soil. This study provides insight into the biodegradation mechanism at the gene level in soil bioelectrochemical remediation.

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Construction of conductive network using magnetite to enhance microflora interaction and petroleum hydrocarbons removal in plant-rhizosphere microbial electrochemical system

Cited by 12 publications

References 62 publications

Exogenous Electroactive Microbes Regulate Soil Geochemical Properties and Microbial Communities by Enhancing the Reduction and Transformation of Fe(III) Minerals

Exogenous Electroactive Microbes Regulate Soil Geochemical Properties and Microbial Communities by Enhancing the Reduction and Transformation of Fe(III) Minerals

Enhancing the Anaerobic Biodegradation of Petroleum Hydrocarbons in Soils with Electrically Conductive Materials

Key genes of electron transfer, the nitrogen cycle and tetracycline removal in bioelectrochemical systems

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