Construction of DNA ligase-mimicking nanozymes <i>via</i> molecular imprinting

He, Xinpei; Luo, Qi; Liu, Ying; Guo, Zhanchen; Liu, Zhen

doi:10.1039/d1tb02325j

Cited by 4 publications

(2 citation statements)

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“…[ 32 ] Because MIPs are cost‐ effective and robust, and have a wide substrate adaptive capacity, other MIP‐coated nanozymes have been developed for specific biorecognition. [ 33‐36 ] Strictly speaking, MIPs do not endow nanozymes with intrinsic specificity. However, when considering MIP‐ coated nanozymes as integrative systems, they can be regarded as nanozymes with quasi‐specificity.…”

Section: Strategies To Develop Nanozymes With Intrinsic Catalytic Spe...mentioning

confidence: 99%

On the Specificity of Nanozymes: A Perspective

Wei,

Liu,

Peng

et al. 2024

Chin. J. Chem.

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Comprehensive SummaryWe have compiled eight promising strategies for enhancing the specificity and selectivity of nanozymes, as depicted in the comprehensive summary above. Enzymes exhibit intricate and sophisticated structures, including substrate channels and active sites, which can inform the design of nanozymes. Replication of these structural features and the application of facet engineering/doping techniques can significantly enhance the catalytic specificity of nanozymes. Alternatively, the use of Molecularly Imprinted Polymers (MIPs) to coat nanozymes represents an effective approach to impart substrate specificity. Furthermore, several straightforward stopgap strategies have been devised to improve nanozyme specificity for analytical applications, such as the integration of biorecognition elements and nanozyme sensor arrays through surface modification.imageKey Scientistsimage

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Section: Strategies To Develop Nanozymes With Intrinsic Catalytic Spe...mentioning

confidence: 99%

On the Specificity of Nanozymes: A Perspective

Wei,

Liu,

Peng

et al. 2024

Chin. J. Chem.

View full text Add to dashboard Cite

show abstract

“… 14 Epoxidation of alkanes using MIP-based catalysts has also been studied with exciting results. 15 Recently, a few examples of the synthesis of enzyme-like MIP nanoparticles capable of catalyzing various reactions, including ligation of short ssDNA fragments, 16 ester hydrolysis, 17 and oxidation of cholesterol derivatives, 18 have been reported. Moreover, hydrophobic cores in polymer micelles containing catalysts have been applied for molecular recognition-based catalysis.…”

Section: Introductionmentioning

confidence: 99%

Improving the Selectivity of the C–C Coupled Product Electrosynthesis by Using Molecularly Imprinted Polymer─An Enhanced Route from Phenol to Biphenol

Sudagar,

Shao,

Żołek

et al. 2023

ACS Appl. Mater. Interfaces

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We developed a procedure for selective 2,4dimethylphenol, DMPh, direct electro-oxidation to 3,3′,5,5′tetramethyl-2,2′-biphenol, TMBh, a C−C coupled product. For that, we used an electrode coated with a product-selective molecularly imprinted polymer (MIP). The procedure is reasonably selective toward TMBh without requiring harmful additives or elevated temperatures. The TMBh product itself was used as a template for imprinting. We followed the template interaction with various functional monomers (FMs) using density functional theory (DFT) simulations to select optimal FM. On this basis, we used a prepolymerization complex of TMBh with carboxyl-containing FM at a 1:2 TMBh-to-FM molar ratio for MIP fabrication. The template−FM interaction was also followed by using different spectroscopic techniques. Then, we prepared the MIP on the electrode surface in the form of a thin film by the potentiodynamic electropolymerization of the chosen complex and extracted the template. Afterward, we characterized the fabricated films by using electrochemistry, FTIR spectroscopy, and AFM, elucidating their composition and morphology. Ultimately, the DMPh electro-oxidation was performed on the MIP film-coated electrode to obtain the desired TMBh product. The electrosynthesis selectivity was much higher at the electrode coated with MIP film in comparison with the reference nonimprinted polymer (NIP) film-coated or bare electrodes, reaching 39% under optimized conditions. MIP film thickness and electrosynthesis parameters significantly affected the electrosynthesis yield and selectivity. At thicker films, the yield was higher at the expense of selectivity, while the electrosynthesis potential increase enhanced the TMBh product yield. Computer simulations of the imprinted cavity interaction with the substrate molecule demonstrated that the MIP cavity promoted direct coupling of the substrate to form the desired TMBh product.

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Nanozymes and Their Potential Roles in the Origin of Life

Ma,

Liang,

Hou

et al. 2024

Advanced Materials

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The origin of life has long been a central scientific challenge, with various hypotheses proposed. The chemical evolution, which supposes that inorganic molecules can transform into organic molecules and subsequent primitive cells, laid the foundation for modern theories. Inorganic minerals are believed to play crucial catalytic roles in the process. However, the harsh reaction conditions of inorganic minerals hinder the accumulation of organic molecules, preventing the efficient transition from inorganic molecules to biomacromolecules. Given the inherent physicochemical properties and enzyme‐like activities, this study proposes that nanozymes, nanomaterials with enzyme‐like activities, act as efficient prebiotic catalysts in the origin of life. This hypothesis is based on the following: First, unlike traditional minerals, nanominerals can catalyze organic synthesis under milder conditions. Second, nanominerals can not only protect biomolecules from radiation damage but also catalyze polymerization reactions to form functional biomacromolecules and further lipid vesicles. More importantly, nanominerals are abundant in terrestrial and extraterrestrial environments. This perspective will systematically discuss the potential roles of nanozymes in the emergence of life based on the functions of minerals and the characteristics of nanozymes. We hope the research on nanozymes and the origin of life will bridge the gap between inorganic precursors and biomolecules under primitive environments.

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Construction of DNA ligase-mimicking nanozymes via molecular imprinting

Abstract: Enzyme mimics are of significant importance due to their facile preparation, low cost and stability to rigorous environment. Molecularly imprinted polymers (MIPs) have been important synthetic mimics of enzymes. However,...

Cited by 4 publications

References 65 publications

On the Specificity of Nanozymes: A Perspective

On the Specificity of Nanozymes: A Perspective

Improving the Selectivity of the C–C Coupled Product Electrosynthesis by Using Molecularly Imprinted Polymer─An Enhanced Route from Phenol to Biphenol

Nanozymes and Their Potential Roles in the Origin of Life

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