Construction of DNA Tools for Hyperexpression in <i>Marchantia</i> Chloroplasts

Frangedakis, Eftychios; Chavez, Fernando Guzman; Rebmann, Marius; Markel, Kasey; Yu, Ying; Perraki, Artemis; Tse, Sze Wai; Liu, Yang; Rever, Jenna; Sauret-Güeto, Susanna; Goffinet, Bernard; Schneider, Harald; Haseloff, Jim

doi:10.1021/acssynbio.0c00637

Cited by 19 publications

(21 citation statements)

References 61 publications

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“…Examination of the annotation of the previously published plastome of Cyathodium smaragdinum (GenBank no. MW429509; Frangedakis et al., 2021) showed that rbcL had two RNA editing sites, whereas ndhA , ndhl , psbB , rpl12 , rps18 and ycf4 had one RNA editing site each. We identified identical nucleotide bases in the RNA editing positions in two of the Cyathodium plastomes sequenced herein.…”

Section: Discussionmentioning

confidence: 99%

New insights into the phylogeny of the complex thalloid liverworts (Marchantiopsida) based on chloroplast genomes

et al. 2022

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Marchantiopsida (complex thalloid liverworts) are one of the earliest lineages of embryophytes (land plants), and well‐known for their air pores and chambers, pegged rhizoids, and absence of organellular RNA editing sites. Despite their importance to an understanding of early embryophyte evolution, many key nodes within this class remain poorly resolved, owing to the paucity of genetic loci previously available for phylogenetic analyses. Here, we sequenced 54 plastomes, representing 28 genera, nearly all families, and all orders of Marchantiopsida. Based on these plastomes, we present a hypothesis of deep relationships within the class, and make the first investigations of gene contents and synteny. Overall, the Marchantiopsida plastomes were well‐conserved, with the exception of the genus Cyathodium that has plastomes with higher GC content, fewer single sequence repeats (SSRs), and more structural variations, implying that this genus might possess RNA editing sites. Abundant repetitive elements and six highly divergent regions were identified as suitable for future infrafamilial taxonomic studies. The phylogenetic topology of Sphaerocarpales, Neohodgsoniales and Blasiales within Marchantiopsida was essentially congruent with previous studies but generally we obtained higher support values. Based on molecular evidence and previous morphological studies, we include Lunulariales in Marchantiales and suggest the retention of narrowed delimitation of monotypic families. The phylogenetic relationships within Marchantiales were better resolved, and 13 monophyletic families were recovered. Our analyses confirmed that the loss of intron 2 of ycf3 is a synapomorphy of Marchantiidae. Finally, we propose a new genus, Asterellopsis (Aytoniaceae), and present an updated classification of Marchantiopsida. The highly supported phylogenetic backbone provided here establishes a framework for future comparative and evolutionary studies of the complex thalloid liverworts.

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Section: Discussionmentioning

confidence: 99%

New insights into the phylogeny of the complex thalloid liverworts (Marchantiopsida) based on chloroplast genomes

et al. 2022

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“…Eventually controlling polyplastidy could facilitate applications such as the engineering of C4 traits in C3 crop species (e.g. to increase the chloroplast compliment in bundle sheath cells ( Schuler et al., 2016 ; Stata et al., 2016 )), manipulating plastid type interchange ( Jarvis and López-Juez, 2013 ; Daniell et al., 2016 ; Solymosi et al., 2018 ) or producing morphologically-tailored plastids optimized for synthetic biology and protein biosynthesis ( Daniell et al., 2016 ; Frangedakis et al., 2021c ). Hornworts therefore represent a key system to study the regulatory mechanisms of chloroplast division, size and segregation, a “rosetta stone” for understanding the evolution of polyplastidy in land plants.…”

Section: The Unique Biology Of Hornwortsmentioning

confidence: 99%

What can hornworts teach us?

et al. 2023

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The hornworts are a small group of land plants, consisting of only 11 families and approximately 220 species. Despite their small size as a group, their phylogenetic position and unique biology are of great importance. Hornworts, together with mosses and liverworts, form the monophyletic group of bryophytes that is sister to all other land plants (Tracheophytes). It is only recently that hornworts became amenable to experimental investigation with the establishment of Anthoceros agrestis as a model system. In this perspective, we summarize the recent advances in the development of A. agrestis as an experimental system and compare it with other plant model systems. We also discuss how A. agrestis can help to further research in comparative developmental studies across land plants and to solve key questions of plant biology associated with the colonization of the terrestrial environment. Finally, we explore the significance of A. agrestis in crop improvement and synthetic biology applications in general.

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“…Sporelings were harvested after 4.5 weeks of growth and the tissues were preserved at À80°C. gDNA extraction was performed using a modified CTAB-based protocol (Frangedakis, 2019). Insert sites were identified using a thermal asymmetric interlaced PCR-based method (TAIL-PCR) (Liu et al, 1995) based on Grotewold, 2003.…”

Section: Spore Productionmentioning

confidence: 99%

An enhancer trap system to track developmental dynamics in Marchantia polymorpha

et al. 2023

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SUMMARYA combination of streamlined genetics, experimental tractability and relative morphological simplicity compared to vascular plants makes the liverwort Marchantia polymorpha an ideal model system for studying many aspects of plant biology. Here we describe a transformation vector combining a constitutive fluorescent membrane marker with a nuclear marker that is regulated by nearby enhancer elements and use this to produce a library of enhancer trap lines for Marchantia. Screening gemmae from these lines allowed the identification and characterization of novel marker lines, including markers for rhizoids and oil cells. The library allowed the identification of a margin tissue running around the thallus edge, highlighted during thallus development. The expression of this marker is correlated with auxin levels. We generated multiple markers for the meristematic apical notch region, which have different spatial expression patterns, reappear at different times during meristem regeneration following apical notch excision and have varying responses to auxin supplementation or inhibition. This reveals that there are proximodistal substructures within the apical notch that could not be observed otherwise. We employed our markers to study Marchantia sporeling development, observing meristem emergence as defining the protonema‐to‐prothallus stage transition, and subsequent production of margin tissue during the prothallus stage. Exogenous auxin treatment stalls meristem emergence at the protonema stage but does not inhibit cell division, resulting in callus‐like sporelings with many rhizoids, whereas pharmacologically inhibiting auxin synthesis and transport does not prevent meristem emergence. This enhancer trap system presents a useful resource for the community and will contribute to future Marchantia research.

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Construction of DNA Tools for Hyperexpression in Marchantia Chloroplasts

Cited by 19 publications

References 61 publications

New insights into the phylogeny of the complex thalloid liverworts (Marchantiopsida) based on chloroplast genomes

New insights into the phylogeny of the complex thalloid liverworts (Marchantiopsida) based on chloroplast genomes

What can hornworts teach us?

An enhancer trap system to track developmental dynamics in Marchantia polymorpha

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