Construction of Genomic Library and High-Throughput Screening of <i>Pseudomonas aeruginosa</i> Novel Antigens for Potential Vaccines

Abstract

Hospital-acquired infections with Pseudomonas aeruginosa have become a great challenge in caring for critically ill and immunocompromised patients. The cause of high mortality is the presence of multi-drug resistant (MDR) strains, which confers a pressing need for vaccines. Although vaccines against P. aeruginosa have been in development for more than several decades, there is no vaccine for patients at present. In this study, we purified genomic DNA of P. aeruginosa from sera of patients affected, constructed genome-wide library with random recombinants, and screened candidate protein antigens by evaluating their protective effects in vivo. After 13-round of screening, 115 reactive recombinants were obtained, among which 13 antigens showed strong immunoreactivity (more than 10% reaction to PcrV, a well-characterized V-antigen of P. aeruginosa). These 13 antigens were: PpiA,

Secretory System Components as Potential Prophylactic Targets for Bacterial Pathogens

“…Search for the most immunogenic proteins was performed by Xu et al [245]. The group used a random screen of genomic library constructs of P. aeruginosa strains isolated from convalescent patients fused with maltose-binding protein (MBP) to select the most immunogenic proteins.…”