Abstract:AIMTo prepare a Gpm6a/ReelinGFPCreERT2 construct with a rapid and reliable strategy using a bacterial artificial chromosome (BAC).METHODSGpm6a and Reelin BACs were purified and transformed into SW102 E. coli by electroporation. The GFPCreERT2 fragment was prepared from a shuttle vector and transformed into SW102 E. coli carrying a BAC. Homologous recombination was induced in SW102 E. coli. Recombinant clones were screened and confirmed by PCR and restriction enzyme digestion. Recombinant clones were transforme… Show more
“…After discussion and agreement among all the authors, it was decided to retract the above article[1] for further consideration due to some misunderstandings in communication.…”
“…After discussion and agreement among all the authors, it was decided to retract the above article[1] for further consideration due to some misunderstandings in communication.…”
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