Construction of introgression lines of Oryza rufipogon and evaluation of important agronomic traits

Qin, Guannan; Nguyen, Hung Manh; Luu, Sinh Ngoc; Wang, Yanwei; Zhang, Zhiguo

doi:10.1007/s00122-018-3241-0

Cited by 8 publications

(5 citation statements)

References 42 publications

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“…While more introgression segments and higher genomic coverage, especially the long fragments, the noise and epistasis effects were effectively reduced, which improved the reliability of identifying major and stronger effective loci that can be directly applied into breeding in the future. Similar conclusion in previous reports just had a brief description [31,50,51]. However, correlations between phenotypes may indicate that complex quantitative traits are controlled by same gene or closely linked genes.…”

Section: Cssls Constructed a Platform For Resolving The Polygene Hyposupporting

confidence: 88%

Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton

Zhu

Wang

et al. 2020

BMC Genomics

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Background: The low genetic diversity of Upland cotton limits the potential for genetic improvement. Making full use of the genetic resources of Sea-island cotton will facilitate genetic improvement of widely cultivated Upland cotton varieties. The chromosome segments substitution lines (CSSLs) provide an ideal strategy for mapping quantitative trait loci (QTL) in interspecific hybridization. Results: In this study, a CSSL population was developed by PCR-based markers assisted selection (MAS), derived from the crossing and backcrossing of Gossypium hirsutum (Gh) and G. barbadense (Gb), firstly. Then, by whole genome re-sequencing, 11,653,661 high-quality single nucleotide polymorphisms (SNPs) were identified which ultimately constructed 1211 recombination chromosome introgression segments from Gb. The sequencing-based physical map provided more accurate introgressions than the PCR-based markers. By exploiting CSSLs with mutant morphological traits, the genes responding for leaf shape and fuzz-less mutation in the Gb were identified. Based on a high-resolution recombination bin map to uncover genetic loci determining the phenotypic variance between Gh and Gb, 64 QTLs were identified for 14 agronomic traits with an interval length of 158 kb to 27 Mb. Surprisingly, multiple alleles of Gb showed extremely high value in enhancing cottonseed oil content (SOC). Conclusions: This study provides guidance for studying interspecific inheritance, especially breeding researchers, for future studies using the traditional PCR-based molecular markers and high-throughput re-sequencing technology in the study of CSSLs. Available resources include candidate position for controlling cotton quality and quantitative traits, and excellent breeding materials. Collectively, our results provide insights into the genetic effects of Gb alleles on the Gh, and provide guidance for the utilization of Gb alleles in interspecific breeding.

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Section: Cssls Constructed a Platform For Resolving The Polygene Hyposupporting

confidence: 88%

Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton

Zhu

Wang

et al. 2020

BMC Genomics

View full text Add to dashboard Cite

show abstract

“…While more introgression segments and higher genomic coverage, especially the long fragments, the noise and epistasis effects were effectively reduced, which improved the reliability of identifying major and stronger effective loci that can be directly applied into breeding in the future. Similar conclusion in previous reports just had a brief description [31, 50,51]. However, correlations between phenotypes may indicate that complex quantitative traits are controlled by same gene or closely linked genes.…”

Section: Cssls Constructed a Platform For Resolving The Polygene Hyposupporting

confidence: 88%

Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton

Zhu

Wang

et al. 2020

Preprint

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Background: The low genetic diversity of Upland cotton limits the potential for genetic improvement. Making full use of the genetic resources of Sea-island cotton will facilitate genetic improvement of widely cultivated Upland cotton varieties. The chromosome segments substitution lines (CSSLs) provide an ideal strategy for mapping quantitative trait loci (QTL) in interspecific hybridization.Results: In this study, a CSSL population was developed by PCR-based markers assisted selection (MAS), derived from the crossing and backcrossing of Gossypium hirsutum (Gh) and G. barbadense (Gb), firstly. Then, by whole genome re-sequencing, 11,653,661 high-quality single nucleotide polymorphisms (SNPs) were identified which ultimately constructed 1,211 recombination chromosome introgression segments from Gb. The sequencing-based physical map provided more accurate introgressions than the PCR-based markers. By exploiting CSSLs with mutant morphological traits, the genes responding for leaf shape and fuzz-less mutation in the Gb were identified. Based on a high-resolution recombination bin map to uncover genetic loci determining the phenotypic variance between Gh and Gb, 64 QTLs were identified for 14 agronomic traits with an interval length of 158 kb to 27 Mb. Surprisingly, multiple alleles of Gb showed extremely high value in enhancing cottonseed oil content (SOC). Conclusions: This study provides guidance for studying interspecific inheritance, especially breeding researchers, for future studies using the traditional PCR-based molecular markers and high-throughput re-sequencing technology in the study of CSSLs. Available resources include candidate position for controlling cotton quality and quantitative traits, and excellent breeding materials. Collectively, our results provide insights into the genetic effects of Gb alleles on the Gh, and provide guidance for the utilization of Gb alleles in interspecific breeding.

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“…For example, the S. pennellii IL population [34] allowed so far the identification of almost 3000 putative QTLs for different traits, like morphology, stress tolerance, yield, fruit colour, and bioactive compounds [37,[51][52][53][54]. The screening of ILs led to a plethora of QTL identification studies in many important crops, like rice, maize, and barley, among others, and for a wide variety of different traits [55][56][57][58][59][60]. Detection of stable QTLs, i.e., those that are detected in different environments, is particularly relevant for breeders, as its introgression allows genetic advances irrespective of the existence of G × E interaction [61].…”

Section: Introductionmentioning

confidence: 99%

Performance of a Set of Eggplant (Solanum melongena) Lines With Introgressions From Its Wild Relative S. incanum Under Open Field and Screenhouse Conditions and Detection of QTLs

et al. 2020

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Introgression lines (ILs) of eggplant (Solanum melongena) represent a resource of high value for breeding and the genetic analysis of important traits. We have conducted a phenotypic evaluation in two environments (open field and screenhouse) of 16 ILs from the first set of eggplant ILs developed so far. Each of the ILs carries a single marker-defined chromosomal segment from the wild eggplant relative S. incanum (accession MM577) in the genetic background of S. melongena (accession AN-S-26). Seventeen agronomic traits were scored to test the performance of ILs compared to the recurrent parent and of identifying QTLs for the investigated traits. Significant morphological differences were found between parents, and the hybrid was heterotic for vigour-related traits. Despite the presence of large introgressed fragments from a wild exotic parent, individual ILs did not display differences with respect to the recipient parent for most traits, although significant genotype × environment interaction (G × E ) was detected for most traits. Heritability values for the agronomic traits were generally low to moderate. A total of ten stable QTLs scattered across seven chromosomes was detected. For five QTLs, the S. incanum introgression was associated with higher mean values for plant- and flower-related traits, including vigour prickliness and stigma length. For one flower- and four fruit-related-trait QTLs, including flower peduncle and fruit pedicel lengths and fruit weight, the S. incanum introgression was associated with lower mean values for fruit-related traits. Evidence of synteny to other previously reported in eggplant populations was found for three of the fruit-related QTLs. The other seven stable QTLs are new, demonstrating that eggplant ILs are of great interest for eggplant breeding under different environments.

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Construction of introgression lines of Oryza rufipogon and evaluation of important agronomic traits

Cited by 8 publications

References 42 publications

Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton

Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton

Genetic dissection of an allotetraploid interspecific CSSLs guides interspecific genetics and breeding in cotton

Performance of a Set of Eggplant (Solanum melongena) Lines With Introgressions From Its Wild Relative S. incanum Under Open Field and Screenhouse Conditions and Detection of QTLs

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