Construction of Metagenomic DNA Libraries and Enrichment Strategies

Benbelgacem, Farah Fadwa; Salleh, Hamzah Mohd.; Noorbatcha, Ibrahim Ali

doi:10.1007/978-981-13-2257-0_3

Cited by 1 publication

(1 citation statement)

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“…In order to increase the possibility of extracting NP complete gene clusters during the construction of metagenomic libraries, the library size and gene cluster structure need to be considered carefully before library construction. Thus, methods divided as direct or indirect extraction procedures for separating DNA from environmental samples like soils/sediments have been developed [ 31 ]. Direct DNA isolation methods depend on the in situ lysis of microbial cells in the specimens before retrieval and extraction of DNA.…”

Section: Function-based Metagenomics For Exploration Of Nps From Different Environmental Samplesmentioning

confidence: 99%

Strategies for Natural Products Discovery from Uncultured Microorganisms

et al. 2021

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Microorganisms are highly regarded as a prominent source of natural products that have significant importance in many fields such as medicine, farming, environmental safety, and material production. Due to this, only tiny amounts of microorganisms can be cultivated under standard laboratory conditions, and the bulk of microorganisms in the ecosystems are still unidentified, which restricts our knowledge of uncultured microbial metabolism. However, they could hypothetically provide a large collection of innovative natural products. Culture-independent metagenomics study has the ability to address core questions in the potential of NP production by cloning and analysis of microbial DNA derived directly from environmental samples. Latest advancements in next generation sequencing and genetic engineering tools for genome assembly have broadened the scope of metagenomics to offer perspectives into the life of uncultured microorganisms. In this review, we cover the methods of metagenomic library construction, and heterologous expression for the exploration and development of the environmental metabolome and focus on the function-based metagenomics, sequencing-based metagenomics, and single-cell metagenomics of uncultured microorganisms.

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Section: Function-based Metagenomics For Exploration Of Nps From Different Environmental Samplesmentioning

confidence: 99%