Construction of potential bacterial consortia for efficient hydrocarbon degradation

Poddar, Kasturi; Sarkar, Debapriya; Sarkar, Angana

doi:10.1016/j.ibiod.2019.104770

Cited by 49 publications

(20 citation statements)

References 56 publications

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“…Oil spills caused by human activities such as offshore oil exploration and marine oil transportation result in serious damage to the marine ecological environment 1 . Currently, the traditional approaches for removing marine spilled oil involve physical and chemical methods.…”

Section: Introductionmentioning

confidence: 99%

“…When the members of a successful bacterial consortium are highly dependent on each other, they form a more stable relationship with each other and maintain cell growth and perform functions more effectively. An increasing number of researchers have focused on constructing oil-degrading consortia to solve the problem of low degradation efficiency by an individual strain 1 . However, the bottleneck in constructing microbial consortia with great application potential is the lack of a reasonable design for the set of members in a consortium 14 .…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies have isolated bacterial strains from soil contaminated with petroleum hydrocarbons and used these strains to establish oil-degrading consortia based on the survival of strains and their ability to degrade hydrocarbons 1 , 15 . However, these consortia still present certain challenges, such as complex construction procedures and unsatisfactory degradation effects.…”

Section: Introductionmentioning

confidence: 99%

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Reconstruction and evaluation of oil-degrading consortia isolated from sediments of hydrothermal vents in the South Mid-Atlantic Ridge

Zheng

Yin

et al. 2021

Sci Rep

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In this study, sediments were collected from two different sites in the deep-sea hydrothermal region of the South Atlantic Ocean. Two microbial enrichment cultures (H7S and H11S), which were enriched from the sediments collected at two sample sites, could effectively degrade petroleum hydrocarbons. The bacterial diversity was analyzed by high-throughput sequencing method. The petroleum degradation ability were evaluated by gas chromatography–mass spectrometry and gravimetric analysis. We found that the dominant oil-degrading bacteria of enrichment cultures from the deep-sea hydrothermal area belonged to the genera Pseudomonas, Nitratireductor, Acinetobacter, and Brevundimonas. After a 14-day degradation experiment, the enrichment culture H11S, which was obtained near a hydrothermal vent, exhibited a higher degradation efficiency for alkanes (95%) and polycyclic aromatic hydrocarbons (88%) than the enrichment culture H7S. Interestingly, pristane and phytane as biomarkers were degraded up to 90% and 91% respectively by the enrichment culture H11S, and six culturable oil-degrading bacterial strains were isolated. Acinetobacter junii strain H11S-25, Nitratireductor sp. strain H11S-31 and Pseudomonas sp. strain H11S-28 were used at a density ratio of 95:4:1 to construct high-efficiency oil-degrading consortium H. After a three-day biodegradation experiment, consortium H showed high degradation efficiencies of 74.2% and 65.7% for total alkanes and PAHs, respectively. The degradation efficiency of biomarkers such as pristane and high-molecular-weight polycyclic aromatic hydrocarbons (such as CHR) reached 84.5% and 80.48%, respectively. The findings of this study indicate that the microorganisms in the deep-sea hydrothermal area are potential resources for degrading petroleum hydrocarbons. Consortium H, which was artificially constructed, showed a highly efficient oil-degrading capacity and has significant application prospects in oil pollution bioremediation.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Reconstruction and evaluation of oil-degrading consortia isolated from sediments of hydrothermal vents in the South Mid-Atlantic Ridge

Zheng

Yin

et al. 2021

Sci Rep

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“…On the other hand, the cell-free culture broth of the strain PWN1 showed lower transmittance value in the India Ink test and also produced white colonies in CBB signifying the absence of any such protein layer outside the cell wall (Fig. 1c) [20]. Therefore, the PWN1 strain was considered to be non-pathogenic and safe for further use.…”

Section: Isolation and Characterization Of Pigment-producing Bacteriamentioning

confidence: 98%

Purification and optimization of pink pigment produced by newly isolated bacterial strain Enterobacter sp. PWN1

et al. 2021

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Pigment-producing bacteria were isolated from kitchen wastewaters of the National Institute of Technology, Rourkela. A pink non-virulent bacterial strain PWN1 was selected based on the India Ink Broth and Coomassie Brilliant Blue (R-250) dye assay. According to morphological and biochemical characterization, the strain PWN1was a Gram-negative, rod-shaped, motile, non-coliform bacterium and could utilize only glucose and adonitol as sole carbon source. The pigment was found to be a growth-associated product, and the pigment production was accelerated after 40 h of bacterial culture. Further, 16S rRNA gene-based molecular identification showed its similarity with Enterobacter sp. The pigments were extracted by the solvent extraction method using chloroform and ethanol (3:1). The extracted pigments were then purified through thin-layer chromatography and column chromatography. To maximize pigment production, the culture condition was optimized for maximum biomass production using statistical software Design Expert v13. A quadratic model was structured describing the process efficiently and it suggested a moderate temperature, pH, and a high inoculum concentration which generated biomass of 3.81 ± 0.02 g/L. At optimized condition, 1 L of cell culture produced 3.77 g of biomass which produced a crude pigment of 0.234 g after solvent extraction and 0.131 g after column chromatography, implying a yield of 6.2% for crude pigment and 3.47% for purified pigment from biomass. The yield of the obtained pigment was high enough to draw interest for industrial production, although the application of the pigment is considerable for further study.

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“…The direct contact between the contaminants and the bacteria's cells is essential, due to the low water solubility of hydrocarbons and the involvement of membrane-bound oxygenases in the initial steps of the catabolism of hydrocarbon biodegradation [14]. Cell adhesion to hydrocarbons [20,21] and the emulsification of such compounds by biosurfactants [22][23][24] have been proposed as mechanisms used by hydrocarbonoclastic bacteria to gain access to these organic substrates.…”

Section: Introductionmentioning

confidence: 99%

Strategy to improve crude oil biodegradation in oligotrophic aquatic environments: W/O/W fertilized emulsions and hydrocarbonoclastic bacteria

et al. 2020

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We studied petroleum biodegradation by biostimulation by using water in oil in water (W/O/W) double emulsions. These emulsions were developed using seawater, canola oil, surfactants, and mineral salts as sources of NPK. The emulsions were used in the simulation of hydrocarbon bioremediation in oligotrophic sea water. Hydrocarbon biodegradation was evaluated by CO 2 emissions from microcosms. We also evaluated the release of inorganic nutrients and the stability of the emulsion's droplets. The double emulsions improved CO 2 emission from the microcosms, suggesting the increase in the hydrocarbon biodegradation. Mineral nutrients were gradually released from the emulsions supporting the hydrocarbon biodegradation. This was attributed to the formation of different diameters of droplets and therefore, varying stabilities of the droplets. Addition of the selected hydrocarbonoclastic isolates simulating bioaugmentation improved the hydrocarbon biodegradation. We conclude that the nutrient-rich W/O/W emulsion developed in this study is an effective biostimulation agent for bioremediation in oligotrophic aquatic environments.

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Construction of potential bacterial consortia for efficient hydrocarbon degradation

Cited by 49 publications

References 56 publications

Reconstruction and evaluation of oil-degrading consortia isolated from sediments of hydrothermal vents in the South Mid-Atlantic Ridge

Reconstruction and evaluation of oil-degrading consortia isolated from sediments of hydrothermal vents in the South Mid-Atlantic Ridge

Purification and optimization of pink pigment produced by newly isolated bacterial strain Enterobacter sp. PWN1

Strategy to improve crude oil biodegradation in oligotrophic aquatic environments: W/O/W fertilized emulsions and hydrocarbonoclastic bacteria

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