Construction of reshaped human antibodies with HIV-neutralizing activity

Humanization and Epitope Mapping of Neutralizing Anti-Human Fas Ligand Monoclonal Antibodies: Structural Insights into Fas/Fas Ligand Interaction

“…The HindIIIBamHI fragments of humanized NOK2 V H and V L cDNAs were then transferred to HindIII-BamHI sites of pCAG-␥1 and pCAG-, respectively. pCAG-␥1 is a mammalian expression vector modified from HCMV-VH0.5␤-␥1 (23) in which the CMV promoter was replaced with the chicken ␤-actin promoter and rabbit ␤-globin splicing acceptor. pCAGwas generated by replacing the human ␥1 constant region in pCAG-␥1 with the constant region.…”

Properties of Whole Human IgG Molecules Produced by the Expression of Cloned Anti-DNA Antibody cDNA in Mammalian Cells

“…Expression was carried out in COS cells as previously described [24,25]. The recombinant heavy chain vector (10-25 g) and the same quantity of recombinant light chain vector were added to 10 7 COS cells in 700 l PBS.…”

Humanization Strategies

“…The two approaches have been compared -in one case, showing no difference in binding affinities [3] and, in the other case, showing better binding with individual frameworks [95]. The fixed-framework approach versus using consensus human frameworks was tested by Maeda et al [96]. The fixed-framework humanized antibodies showed loss of binding while the consensus-framework antibodies did not.…”