Construction of species-specific primers for Pseudomonas andropogonis based on 16S rDNA sequences

Abstract

Approximately 94% of the total 16S rDNA of Pseudomonas andropogonis strain ACH 01053A was sequenced and compared with that of strain ATCC 23061 obtained from the GenBank database. The two sequences were highly homologous with 1.3% difference. Alignment of sequences with those from closely related bacteria revealed two possible regions for design of a specific primer suitable for detection of Ps. andropogonis. Using primers designed to these variable regions in a PCR test, an amplification product of approximately 410 bp was specifically produced by 40 strains of Ps. andropogonis. No other bacterial species showed an amplification product under optimized PCR conditions. As few as 1000 cells per reaction were detected.

Use of ribotyping and random amplified polymorphic DNA to differentiate isolates of Burkholderia andropogonis

“…The isolates of B. andropogonis used in this study are listed in Table 1. All isolates were confirmed as B. andropogonis using the species‐specific primer pair Pf and Pr (Bagsic et al . 1995).…”

Bacterial black spot caused by Burkholderia andropogonis on Odontoglossum and intergeneric hybrid orchids

“…They reacted entirely the same as the 17 isolates in API 20NE tests. All 17 isolates were further confirmed as B. andropogonis by PCR using the species-specific primer pair Pf and Pr, which correspond to 16S rDNA (Bagsic et al 1995). Only an approximately 400-bp fragment of DNA of the expected size was amplified from the extract of each isolate.…”

Mancha bacteriana em Ruscus sp. causada por Burkholderia andropogonis no Brasil

“…AG1051. com 20 dias de idade, ambos hospedeiros de B. andropogonis (Bagsic et al, 1995), utilizando-se a mesma metodologia. Nas plantas testemunhas, o inóculo foi substituído por água esterilizada.…”

“…B. andropogonis apresenta um grande número de plantas hospedeiras, algumas de importância econômica, incluindo 15 famílias e 51 espécies entre mono e dicotiledôneas. Esse patógeno se apresenta amplamente distribuído em diversas regiões da África, Ásia, Austrália, Oceania, Europa e Américas do Norte e do Sul (Bagsic et al, 1995).…”

“…Essa espécie bacteriana apresenta relativa homogeneidade com relação à filogenia e características fenotípicas, mas grande diversidade genética entre isolados, sem que tenha sido observada correlação entre hospedeiro, localização geográfica e patogenicidade (Bagsic et al, 1995;Bagsic-Opulencia et al, 2001). No Brasil, esta bactéria já foi descrita causando danos em primavera (Bougainvillea sp.…”