Construction of Swine-Specific CpG Motif Enriched Plasmid and the Study of Its Immunostimulatory Effects Both &lt;i&gt;In Vitro&lt;/i&gt; and &lt;i&gt;In Vivo&lt;/i&gt;

Guo, Xiaoyu; Ji, Hong; Yuan, Weifeng; Zhang, Quan; Hou, Shaohua; Zhu, Gang; Zhu, Hongfei; Sánchez-Vizcaíno, José Manuel

doi:10.1292/jvms.11-0543

Cited by 2 publications

(1 citation statement)

References 25 publications

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“…Splenocytes were covered in 96-well microtiter plate (Corning Inc., Corning, NY, USA) at a density of 5 £ 10 5 cell per well, and were stimulated with HEV recombinant polypeptide vaccine (40 mg/ml) or 1640 medium (Corning Inc., Corning, NY, USA) (negative control), respectively, then incubated for 68 h at 37 C, then added 40 ml MTS (CellTiter 96 Ò AQ ueous One Soultion Cell Proliferation Assay, Promega, Madison, WI, USA) per well and incubated for another 4 h at 37 C. And the absorbance of light at 490 nm wavelength was measured using a Multiskan MK3 Microplate Reader. Stimulation index (SI) was calculated as described by Guo et al 30 SI D OD sample ¡ OD blank 6 OD negative ¡ OD blank .…”

Section: Splenocytes Proliferation Analysismentioning

confidence: 99%

N-(2-hydroxy) propyl-3-trimethylammonium chitosan chloride: An immune-enhancing adjuvant for hepatitis E virus recombinant polypeptide vaccine in mice

Wei

Zheng

et al. 2017

Human Vaccines & Immunotherapeutics

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Adjuvants are essential for enhancing vaccine potency by improving the humoral and/or cell-mediated immune response to vaccine antigens. This study was performed to evaluate the immuno-enhancing characteristic of N-(2-hydroxy) propyl-3-trimethylammonium chitosan chloride (HTCC), the cationically modified chitosan, as an adjuvant for hepatitis E virus (HEV) recombinant polypeptide vaccine. Animal experiments showed that HTCC provides adjuvant activity when co-administered with HEV recombinant polypeptide vaccine by intramuscularly route. Vaccination using HTCC as an adjuvant was associated with increases of the serum HEV-specific IgG antibodies, splenocytes proliferation and the growths of CD4CD8 T lymphocytes and IFN-γ-secreting T lymphocytes in peripheral blood. These findings suggested that HTCC had strong immuno-enhancing effect. Our findings are the first to demonstrate that HTCC is safe and effective in inducing a good antibody response and stimulating Th1-biased immune responses for HEV recombinant polypeptide vaccine.

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Section: Splenocytes Proliferation Analysismentioning

confidence: 99%

N-(2-hydroxy) propyl-3-trimethylammonium chitosan chloride: An immune-enhancing adjuvant for hepatitis E virus recombinant polypeptide vaccine in mice

Wei

Zheng

et al. 2017

Human Vaccines & Immunotherapeutics

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Evaluation of Immunostimulatory Effects of N-(2-Hydroxy) Propyl-3-Trimethylammonium Chitosan Chloride for Improving Live Attenuated Hepatitis A Virus Vaccine Efficacy

Wei

et al. 2017

Viral Immunology

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This study was to evaluate the immunostimulatory effects of N-(2-hydroxy) propyl-3-trimethylammonium chitosan chloride (HTCC) as an adjuvant for improving a commercial live attenuated hepatitis A virus (HAV) vaccine efficacy in mice. Mice in the experimental group were intraperitoneally immunized with a solution of HTCC and live attenuated HAV vaccine. And for those injected with sterile water, HTCC or live attenuated HAV vaccine were treated as mock group, negative group, and positive group in turn. The serum HAV-specific IgG titers and the ratios of the serum HAV-specific IgG2a/IgG1 in the experimental group were significantly increased (p = 0.00042 and p = 0.040, respectively). Splenocyte proliferation stimulation index in experimental group was higher than positive group (p = 0.021), and significantly higher than mock group and negative group (p = 0.0078 and p = 0.0050, respectively). The percentages of CD4 T lymphocytes in the peripheral blood in experimental group were significantly higher than positive group, negative group, and mock group (p = 0.012, p = 0.012, and p = 0.045, respectively). Compared to the other three groups, experimental group showed a slightly higher ratio of CD4/CD8, but there were no significant differences (p > 0.05). In the percentages of CD8 T lymphocytes, there were no significant differences among the four groups (p > 0.05). HTCC can enhance live attenuated HAV vaccine to generate stronger humoral responses and induce a Th1-biased immune response, as well as IgG2a class switching, compared with the live attenuated HAV vaccine alone. This study validated an important concept for further development of a safe and potent vaccine adjuvant.

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Construction of Swine-Specific CpG Motif Enriched Plasmid and the Study of Its Immunostimulatory Effects Both <i>In Vitro</i> and <i>In Vivo</i>

Cited by 2 publications

References 25 publications

N-(2-hydroxy) propyl-3-trimethylammonium chitosan chloride: An immune-enhancing adjuvant for hepatitis E virus recombinant polypeptide vaccine in mice

N-(2-hydroxy) propyl-3-trimethylammonium chitosan chloride: An immune-enhancing adjuvant for hepatitis E virus recombinant polypeptide vaccine in mice

Evaluation of Immunostimulatory Effects of N-(2-Hydroxy) Propyl-3-Trimethylammonium Chitosan Chloride for Improving Live Attenuated Hepatitis A Virus Vaccine Efficacy

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