Construction of Synthetic Dermis and Skin Based on a Self-Assembled Peptide Hydrogel Scaffold

Kao, Bunsho; Kadomatsu, Koichi; Hosaka, Yoshiaki

doi:10.1089/ten.tea.2008.0362

Cited by 24 publications

(14 citation statements)

References 53 publications

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“…A wide range of techniques such as electrospinning, spray deposition, selfassembly, polyelectrolyte multilayer methodologies, and a decellularized ECM have been used to fabricate structures that mimic BM based on natural and synthetic polymers. [25][26][27][28][29] In each organ, a tissue-specific ECM regulates the functionality of epithelial cells. These epithelial cell/ECM interactions need to be replicated for the regeneration of the tissue.…”

Section: Functional Epithelium For Regenerative Medicinementioning

confidence: 99%

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Vrana

Lavalle²,

Dokmeci³

et al. 2013

Tissue Engineering Part B: Reviews

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Recent advances in the fields of microfabrication, biomaterials, and tissue engineering have provided new opportunities for developing biomimetic and functional tissues with potential applications in disease modeling, drug discovery, and replacing damaged tissues. An intact epithelium plays an indispensable role in the functionality of several organs such as the trachea, esophagus, and cornea. Furthermore, the integrity of the epithelial barrier and its degree of differentiation would define the level of success in tissue engineering of other organs such as the bladder and the skin. In this review, we focus on the challenges and requirements associated with engineering of epithelial layers in different tissues. Functional epithelial layers can be achieved by methods such as cell sheets, cell homing, and in situ epithelialization. However, for organs composed of several tissues, other important factors such as (1) in vivo epithelial cell migration, (2) multicell-type differentiation within the epithelium, and (3) epithelial cell interactions with the underlying mesenchymal cells should also be considered. Recent successful clinical trials in tissue engineering of the trachea have highlighted the importance of a functional epithelium for long-term success and survival of tissue replacements. Hence, using the trachea as a model tissue in clinical use, we describe the optimal structure of an artificial epithelium as well as challenges of obtaining a fully functional epithelium in macroscale. One of the possible remedies to address such challenges is the use of bottom-up fabrication methods to obtain a functional epithelium. Modular approaches for the generation of functional epithelial layers are reviewed and other emerging applications of microscale epithelial tissue models for studying epithelial/mesenchymal interactions in healthy and diseased (e.g., cancer) tissues are described. These models can elucidate the epithelial/mesenchymal tissue interactions at the microscale and provide the necessary tools for the next generation of multicellular engineered tissues and organ-on-a-chip systems.

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Section: Functional Epithelium For Regenerative Medicinementioning

confidence: 99%

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Vrana

Lavalle²,

Dokmeci³

et al. 2013

Tissue Engineering Part B: Reviews

View full text Add to dashboard Cite

show abstract

“…support of a wide range of biological applications, including osteoblast proliferation and differentiation, [26,27] axon regeneration, [28] and keratinocyte differentiation. [29] In addition to applications in tissue regeneration, RAD16-I was recently investigated as a 3D model for ovarian cancer cells, and was found to promote 3D cell adhesion and migration. [30] It was found that ovarian cancer cells on the matrix exhibited a higher resistance to anticancer drugs compared to cells grown on a 2D culture plate, allowing for improved anticancer drug screening and in vitro investigation of tumor biology.…”

Section: β-Sheet Forming Peptide Hydrogelsmentioning

confidence: 99%

Self‐Assembling Peptides as Cell‐Interactive Scaffolds

Zhang

Hauser

2011

Adv Funct Materials

140

123

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Furthermore, the information for self-assembly is encoded in the amino acid sequence, providing the ability to tune and form Self-Assembling Peptides as Cell-Interactive ScaffoldsCell and tissue engineering therapies for regenerative medicine as well as cell-based assays require an understanding of the interactions between cells with the surrounding microenvironment at the nanoscale. Engineering a cell-interactive scaffold therefore entails control over the nanostructure of the biomaterial. Peptides that are able to self-assemble into 3D scaffolds have emerged as interesting biomaterials for directing cell behavior, with desirable properties such as the capability of tuning the nanostructure by modulating the amino acid composition. Here, an overview of the development of self-assembling peptide hydrogels as functional cell scaffolds is presented, highlighting recent work on incorporating features such as bioactive ligands, growth factor delivery, controlled degradation, and formulation into microgels for defined cell microenvironments.

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“…[156] Self-assembled peptide nanofibers have also been used to create hydrogels with controlled presentations of RGD and other cell adhesion ligands. [134] Such materials have been used to study bone regeneration therapies[164], culturing of synthetic dermis[165], and tubulogenesis of endothelial cells[166], among other biomedical applications[167]. …”

Section: Investigating Cell Behaviors In 3dmentioning

confidence: 99%

Nanoscale tissue engineering: spatial control over cell-materials interactions

et al. 2011

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Cells interact with the surrounding environment by making tens to hundreds of thousands of nanoscale interactions with extracellular signals and features. The goal of nanoscale tissue engineering is to harness the interactions through nanoscale biomaterials engineering in order to study and direct cellular behaviors. Here, we review the nanoscale tissue engineering technologies for both two- and three-dimensional studies (2- and 3D), and provide a holistic overview of the field. Techniques that can control the average spacing and clustering of cell adhesion ligands are well established and have been highly successful in describing cell adhesion and migration in 2D. Extension of these engineering tools to 3D biomaterials has created many new hydrogel and nanofiber scaffolds technologies that are being used to design in vitro experiments with more physiologically relevant conditions. Researchers are beginning to study complex cell functions in 3D, however, there is a need for biomaterials systems that provide fine control over the nanoscale presentation of bioactive ligands in 3D. Additionally, there is a need for 2- and 3D techniques that can control the nanoscale presentation of multiple bioactive ligands and the temporal changes in cellular microenvironment.

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Construction of Synthetic Dermis and Skin Based on a Self-Assembled Peptide Hydrogel Scaffold

Cited by 24 publications

References 53 publications

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Engineering Functional Epithelium for Regenerative Medicine and In Vitro Organ Models: A Review

Self‐Assembling Peptides as Cell‐Interactive Scaffolds

Nanoscale tissue engineering: spatial control over cell-materials interactions

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