Construction of the lactate-sensing fibremats by confining sensor fluorescent protein of lactate inside nanofibers of the poly(HPMA/DAMA)/ADH-nylon 6 core–shell fibremat

Kato, Yuna; Iwata, Shuichi; Nasu, Yusuke; Obata, Akiko; Nagata, Kenji; Campbell, Robert E.; Mizuno, Toshihisa

doi:10.1039/d3ra06108f

RSC Adv.

2023

DOI: 10.1039/d3ra06108f

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Construction of the lactate-sensing fibremats by confining sensor fluorescent protein of lactate inside nanofibers of the poly(HPMA/DAMA)/ADH-nylon 6 core–shell fibremat

Yuna Kato,

Shuichi Iwata,

Yusuke Nasu

et al.

Abstract: New materials platform, capable of sustaining the functionality of proteinous sensor molecules over an extended period without being affected by proteases.

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2024

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Peroxidase-like Nanoparticles of Noble Metals Stimulate Increasing Sensitivity of Flavocytochrome b2-Based L-Lactate Biosensors

Gayda,

Demkiv,

Stasyuk

et al. 2024

Biosensors

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We report the development of amperometric biosensors (ABSs) employing flavocytochrome b2 (Fcb2) coupled with nanoparticles (NPs) of noble metals on graphite electrode (GE) surfaces. Each NPs/GE configuration was evaluated for its ability to decompose hydrogen peroxide (H2O2), mimicking peroxidase (PO) activity. The most effective nanoPO (nPO) was selected for developing ABSs targeting L-lactate. Consequently, several Fcb2/nPO-based ABSs with enhanced sensitivity to L-lactate were developed, demonstrating mediated ET between Fcb2 and the GE surface. The positive effect of noble metal NPs on Fcb2-based sensor sensitivity may be explained by the synergy between their dual roles as both PO mimetics and electron transfer mediators. Furthermore, our findings provide preliminary data that may prompt a re-evaluation of the mechanism of L-lactate oxidation in Fcb2-mediated catalysis. Previously, it was believed that L-lactate oxidation via Fcb2 catalysis did not produce H2O2, unlike catalysis via L-lactate oxidase. Our initial research revealed that the inclusion of nPO in Fcb2-based ABSs significantly increased their sensitivity. Employing other PO mimetics in ABSs for L-lactate yielded similar results, reinforcing our hypothesis that trace amounts of H2O2 may be generated as a transient intermediate in this reaction. The presence of nPO enhances the L-lactate oxidation rate through H2O2 utilization, leading to signal amplification and heightened bioelectrode sensitivity. The proposed ABSs have been successfully tested on blood serum and fermented food samples, showing their promise for L-lactate monitoring in medicine and the food industry.

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Peroxidase-like Nanoparticles of Noble Metals Stimulate Increasing Sensitivity of Flavocytochrome b2-Based L-Lactate Biosensors

Gayda,

Demkiv,

Stasyuk

et al. 2024

Biosensors

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show abstract

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Construction of the lactate-sensing fibremats by confining sensor fluorescent protein of lactate inside nanofibers of the poly(HPMA/DAMA)/ADH-nylon 6 core–shell fibremat

Abstract: New materials platform, capable of sustaining the functionality of proteinous sensor molecules over an extended period without being affected by proteases.

Cited by 1 publication

References 31 publications

Peroxidase-like Nanoparticles of Noble Metals Stimulate Increasing Sensitivity of Flavocytochrome b2-Based L-Lactate Biosensors

Peroxidase-like Nanoparticles of Noble Metals Stimulate Increasing Sensitivity of Flavocytochrome b2-Based L-Lactate Biosensors

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